Volumetric Properties of Proteins and the Role of Solvent in Conformational Dynamics

2009 ◽  
pp. 173-186
Author(s):  
C. A. Royer ◽  
R. Winter
Keyword(s):  
Conformational Dynamics ◽  
Volumetric Properties ◽  
Role Of Solvent

scholarly journals Tethering-induced destabilization and ATP-binding for tandem RRM domains of ALS-causing TDP-43 and hnRNPA1

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Mei Dang ◽  
Yifan Li ◽  
Jianxing Song
Keyword(s):  
Conformational Dynamics ◽  
Md Simulations ◽  
Atp Binding ◽  
Rna Recognition Motif ◽  
Rna Recognition ◽  
Nucleic Acid Binding ◽  
General Role ◽  
Nmr Characterization ◽  
Lateral Sclerosis

AbstractTDP-43 and hnRNPA1 contain tandemly-tethered RNA-recognition-motif (RRM) domains, which not only functionally bind an array of nucleic acids, but also participate in aggregation/fibrillation, a pathological hallmark of various human diseases including amyotrophic lateral sclerosis (ALS), frontotemporal dementia (FTD), alzheimer's disease (AD) and Multisystem proteinopathy (MSP). Here, by DSF, NMR and MD simulations we systematically characterized stability, ATP-binding and conformational dynamics of TDP-43 and hnRNPA1 RRM domains in both tethered and isolated forms. The results reveal three key findings: (1) upon tethering TDP-43 RRM domains become dramatically coupled and destabilized with Tm reduced to only 49 °C. (2) ATP specifically binds TDP-43 and hnRNPA1 RRM domains, in which ATP occupies the similar pockets within the conserved nucleic-acid-binding surfaces, with the affinity slightly higher to the tethered than isolated forms. (3) MD simulations indicate that the tethered RRM domains of TDP-43 and hnRNPA1 have higher conformational dynamics than the isolated forms. Two RRM domains become coupled as shown by NMR characterization and analysis of inter-domain correlation motions. The study explains the long-standing puzzle that the tethered TDP-43 RRM1–RRM2 is particularly prone to aggregation/fibrillation, and underscores the general role of ATP in inhibiting aggregation/fibrillation of RRM-containing proteins. The results also rationalize the observation that the risk of aggregation-causing diseases increases with aging.

scholarly journals Dye-Surfactant Interaction: Role of Solvent

2013 ◽  
Vol 25 (18) ◽  
pp. 10499-10503 ◽  
Author(s):  
Ziya Ahmad Khan ◽  
Abdullah S. Al-Bogami
Keyword(s):  
Role Of Solvent

Role of Solvent in Excited-State Proton Transfer in Hypericin

1994 ◽  
Vol 98 (34) ◽  
pp. 8352-8358 ◽  
Author(s):  
F. Gai ◽  
M. J. Fehr ◽  
J. W. Petrich
Keyword(s):  
Excited State ◽  
Proton Transfer ◽  
Excited State Proton Transfer ◽  
Role Of Solvent

Asymmetrical growth and dissolution along polar axis of α-resorcinol crystal: role of solvent and external environment

CrystEngComm ◽  
2013 ◽  
Vol 15 (42) ◽  
pp. 8493 ◽  
Author(s):  
M. K. Singh ◽  
S. K. Sharma ◽  
Arup Banerjee
Keyword(s):  
External Environment ◽  
Polar Axis ◽  
Role Of Solvent

The Role of Solvent in Hydrogen Bonding Pattern of Ellagic Acid Crystals

2013 ◽  
Vol 43 (6) ◽  
pp. 285-291 ◽  
Author(s):  
Ewa Żesławska ◽  
Agnieszka Skórska-Stania
Keyword(s):  
Hydrogen Bonding ◽  
Ellagic Acid ◽  
Hydrogen Bonding Pattern ◽  
Role Of Solvent

scholarly journals 155 The role of solvent mobility in protein dynamics

2015 ◽  
Vol 33 (sup1) ◽  
pp. 100-101
Author(s):  
Qiong Yang ◽  
Peng Sang ◽  
Shu-Qun Liu
Keyword(s):  
Protein Dynamics ◽  
Role Of Solvent

ChemInform Abstract: THE ROLE OF SOLVENT IN CONJUGATE ADDITIONS LEADING TO CIS- AND TRANS-1-ALKYL-2-ARYL-3-CARBO(AROYL)AZIRIDINES

1977 ◽  
Vol 8 (40) ◽  
pp. no-no
Author(s):  
P. TARBURTON ◽  
P. B. WOLLER ◽  
R. C. BADGER ◽  
E. DOOMES ◽  
N. H. CROMWELL
Keyword(s):  
Conjugate Additions ◽  
Cis And Trans ◽  
Role Of Solvent

scholarly journals Resolving the complex role of enzyme conformational dynamics in catalytic function

2012 ◽  
Vol 109 (15) ◽  
pp. 5699-5704 ◽  
Author(s):  
U. Doshi ◽  
L. C. McGowan ◽  
S. T. Ladani ◽  
D. Hamelberg
Keyword(s):  
Conformational Dynamics ◽  
Catalytic Function
Sign in / Sign up

Export Citation Format

Share Document