In Vivo Properties of In Vitro-Propagated Neural Stem Cells After Transplantation to the Neonatal and Adult Rat Brain

2004 ◽  
pp. 117-138
Author(s):  
Ulrica Englund ◽  
Anders Bjöklund
Keyword(s):  
Stem Cells ◽  
Neural Stem Cells ◽  
Rat Brain ◽  
Adult Rat ◽  
Adult Rat Brain

Human neural stem cells improve sensorimotor deficits in the adult rat brain with experimental focal ischemia

2004 ◽  
Vol 1016 (2) ◽  
pp. 145-153 ◽  
Author(s):  
Kon Chu ◽  
Manho Kim ◽  
Kyung-Il Park ◽  
Sang-Wuk Jeong ◽  
Hee-Kwon Park ◽  
...  
Keyword(s):  
Stem Cells ◽  
Neural Stem Cells ◽  
Rat Brain ◽  
Focal Ischemia ◽  
Human Neural Stem Cells ◽  
Adult Rat ◽  
Sensorimotor Deficits ◽  
Adult Rat Brain

Identification and early characterization of genetically modified NGF-producing neural stem cells grafted into the injured adult rat brain

2008 ◽  
Vol 30 (3) ◽  
pp. 244-250 ◽  
Author(s):  
Lucia Pagani ◽  
Carlo Cenciarelli ◽  
Patrizia Casalbore ◽  
Manuela Budoni ◽  
Valentina Sposato ◽  
...  
Keyword(s):  
Stem Cells ◽  
Neural Stem Cells ◽  
Rat Brain ◽  
Genetically Modified ◽  
Adult Rat ◽  
Adult Rat Brain

Neurogenesis and neural stem cells in the dorsal vagal complex of adult rat brain: New vistas about autonomic regulations—a review

2006 ◽  
Vol 126-127 ◽  
pp. 50-58 ◽  
Author(s):  
Emmanuel Moyse ◽  
Sylvian Bauer ◽  
Céline Charrier ◽  
Valérie Coronas ◽  
S. Krantic ◽  
...  
Keyword(s):  
Stem Cells ◽  
Neural Stem Cells ◽  
Rat Brain ◽  
Dorsal Vagal Complex ◽  
Adult Rat ◽  
Adult Rat Brain

Characterization of neural stem cells in the dorsal vagal complex of adult rat by in vivo proliferation labeling and in vitro neurosphere assay

Neuroscience ◽  
2006 ◽  
Vol 138 (1) ◽  
pp. 5-16 ◽  
Author(s):  
C. Charrier ◽  
V. Coronas ◽  
J. Fombonne ◽  
M. Roger ◽  
A. Jean ◽  
...  
Keyword(s):  
Stem Cells ◽  
Neural Stem Cells ◽  
Dorsal Vagal Complex ◽  
Adult Rat ◽  
Neurosphere Assay

scholarly journals EXTH-07. TUMOR-HOMING INDUCED NEURAL STEM CELLS SECRETING A CYTOTOXIC PAYLOAD AS AN ADJUVANT TREATMENT FOR NON-SMALL CELL LUNG CANCER BRAIN METASTASES

2020 ◽  
Vol 22 (Supplement_2) ◽  
pp. ii88-ii88
Author(s):  
Alison Mercer-Smith ◽  
Wulin Jiang ◽  
Alain Valdivia ◽  
Juli Bago ◽  
Scott Floyd ◽  
...  
Keyword(s):  
Stem Cells ◽  
Brain Metastases ◽  
Neural Stem Cells ◽  
Adjuvant Treatment ◽  
Small Cell ◽  
Small Cell Lung ◽  
Tumor Homing ◽  
Induced Neural Stem Cells

Abstract INTRODUCTION Non-small cell lung cancer (NSCLC) is the most common cancer to form brain metastases. Radiation treatment is standard-of-care, but recurrence is still observed in 40% of patients. An adjuvant treatment is desperately needed to track down and kill tumor remnants after radiation. Tumoritropic neural stem cells (NSCs) that can home to and deliver a cytotoxic payload offer potential as such an adjuvant treatment. Here we show the transdifferentiation of human fibroblasts into tumor-homing induced neural stem cells (hiNSCs) that secrete the cytotoxic protein TRAIL (hiNSC-TRAIL) and explore the use of hiNSC-TRAIL to treat NSCLC brain metastases. METHODS To determine the migratory capacity of hiNSCs, hiNSCs were infused intracerebroventricularly (ICV) into mice bearing established bilateral NSCLC H460 brain tumors. hiNSC accumulation at tumor foci was monitored using bioluminescent imaging and post-mortem fluorescent analysis. To determine synergistic effects of radiation with TRAIL on NSCLC, we performed in vitro co-culture assays and isobologram analysis. In vivo, efficacy was determined by tracking the progression and survival of mice bearing intracranial H460 treated with hiNSC-TRAIL alone or in combination with 2 Gy radiation. RESULTS/CONCLUSION Following ICV infusion, hiNSCs persisted in the brain for > 1 week and migrated from the ventricles to colocalize with bilateral tumor foci. In vitro, viability assays and isobologram analysis revealed the combination treatment of hiNSC-TRAIL and 2 Gy radiation induced synergistic killing (combination index=0.64). In vivo, hiNSC-TRAIL/radiation combination therapy reduced tumor volumes > 90% compared to control-treated animals while radiation-only and hiNSC-TRAIL-only treated mice showed 21% and 52% reduced volumes, respectively. Dual-treatment extended survival 40%, increasing survival from a median of 20 days in controls to 28 days in the treatment group. These results suggest hiNSC-TRAIL can improve radiation therapy for NSCLC brain metastases and could potentially improve outcomes for patients suffering from this aggressive form of cancer.

scholarly journals Immunosuppressants Affect Human Neural Stem Cells In Vitro but Not in an In Vivo Model of Spinal Cord Injury

2013 ◽  
Vol 2 (10) ◽  
pp. 731-744 ◽  
Author(s):  
Christopher J. Sontag ◽  
Hal X. Nguyen ◽  
Noriko Kamei ◽  
Nobuko Uchida ◽  
Aileen J. Anderson ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Stem Cells ◽  
Spinal Cord Injury ◽  
Neural Stem Cells ◽  
In Vivo Model ◽  
Human Neural Stem Cells ◽  
Cord Injury

In Vitro Metal Inhibition of N-Methyl-D-Aspartate Specific Glutamate Receptor Binding in Neonatal and Adult Rat Brain

1997 ◽  
Vol 20 (1-2) ◽  
pp. 21-29 ◽  
Author(s):  
Bettaiya Rajanna ◽  
Sharada Rajanna ◽  
Elizabeth Hall ◽  
Prabhakara R. Yallapragada
Keyword(s):  
Rat Brain ◽  
Glutamate Receptor ◽  
Receptor Binding ◽  
Adult Rat ◽  
Adult Rat Brain

scholarly journals Could Cells from Your Nose Fix Your Heart? Transplantation of Olfactory Stem Cells in a Rat Model of Cardiac Infarction

2010 ◽  
Vol 10 ◽  
pp. 422-433 ◽  
Author(s):  
Cameron McDonald ◽  
Alan Mackay-Sim ◽  
Denis Crane ◽  
Wayne Murrell
Keyword(s):  
Stem Cells ◽  
Neural Stem Cells ◽  
Fluorescent Protein ◽  
Genetically Engineered ◽  
Olfactory Mucosa ◽  
Cell Transplant ◽  
Adult Rat ◽  
Cardiac Ventricle ◽  
Green Fluorescent

This study examines the hypothesis that multipotent olfactory mucosal stem cells could provide a basis for the development of autologous cell transplant therapy for the treatment of heart attack. In humans, these cells are easily obtained by simple biopsy. Neural stem cells from the olfactory mucosa are multipotent, with the capacity to differentiate into developmental fates other than neurons and glia, with evidence of cardiomyocyte differentiationin vitroand after transplantation into the chick embryo. Olfactory stem cells were grown from rat olfactory mucosa. These cells are propagated as neurosphere cultures, similar to other neural stem cells. Olfactory neurospheres were grownin vitro, dissociated into single cell suspensions, and transplanted into the infarcted hearts of congeneic rats. Transplanted cells were genetically engineered to express green fluorescent protein (GFP) in order to allow them to be identified after transplantation. Functional assessment was attempted using echocardiography in three groups of rats: control, unoperated; infarct only; infarcted and transplanted. Transplantation of neurosphere-derived cells from adult rat olfactory mucosa appeared to restore heart rate with other trends towards improvement in other measures of ventricular function indicated. Importantly, donor-derived cells engrafted in the transplanted cardiac ventricle and expressed cardiac contractile proteins.

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