Multilocus analysis of external pollen contamination of a Scots pine (Pinus sylvestris L.) seed orchard

1985 ◽  
pp. 134-138 ◽  
Author(s):  
Kazutosi Nagasaka ◽  
Alfred E. Szmidt
Keyword(s):  
Pinus Sylvestris ◽  
Scots Pine ◽  
Seed Orchard ◽  
Pollen Contamination ◽  
Multilocus Analysis

scholarly journals Evaluation of Pollen Contamination in an Advanced Scots Pine Seed Orchard

2009 ◽  
Vol 58 (1-6) ◽  
pp. 262-269 ◽  
Author(s):  
T. Torimaru ◽  
Xiao-Ru Wang ◽  
A. Fries ◽  
B. Andersson ◽  
D. Lindgren
Keyword(s):  
Pinus Sylvestris ◽  
Scots Pine ◽  
Genetic Gain ◽  
Seed Orchard ◽  
Multilocus Genotypes ◽  
Pollen Contamination ◽  
Pollen Sources ◽  
Ssr Loci ◽  
The Impact ◽  
Paternity Exclusion

Abstract The pollination pattern in a Scots pine (Pinus sylvestris L.) seed orchard consisting of 28 clones was studied using nine microsatellite (SSR) loci. The nine SSR loci produced unique multilocus genotypes for each of the orchard’s 28 clones and allowed paternal assignment of the studied 305 seed using paternity exclusion probability of 99.9%. Fifty two percent of the studied seeds were sired by outside the orchard pollen sources (i.e., pollen contamination) and as expected, low selfing (2.3%) was detected. These results are valuable for the evaluation of the seed orchard function and the impact of contamination on the expected genetic gain.

scholarly journals Are we narrowing genetic variability in seed orchards? An attempt to answer, based on the analysis of microsatellite DNA of grafts growing in Scots pine (Pinus sylvestris L.) seed orchard in the Forest District Susz

2015 ◽  
Vol 76 (3) ◽  
pp. 240-249
Author(s):  
Paweł Przybylski
Keyword(s):  
Genetic Variability ◽  
Pinus Sylvestris ◽  
Scots Pine ◽  
Microsatellite Dna ◽  
Molecular Genetic ◽  
Seed Orchard ◽  
Dna Fragments ◽  
Forest Stands ◽  
Seed Orchards ◽  
Forest District

Abstract Scots pine (Pinus sylvestris L.) is the most common species in Poland’s forest stands. The mode of pine stands renovation requires that silviculture practitioners have continuous access to seed banks. Orchard-grown seeds are predicted to constitute an increasingly larger part of the average demand for pine seeds in Poland. Seed orchards, due to a limited number of maternal trees as well as the irregularity of their blooming and pollination, enhance the risk of genetic diversity reduction in planted forest stands. This is of particular importance in the context of dynamic climate change. Markers based on microsatellite DNA fragments are effective tools for monitoring genetic variability. In the present study, three different microsatellite DNA fragments were used: SPAC 12.5, SPAG 7.14 and SPAC 11.4. The main objective of this research was to study genetic variability in one of the biggest seed orchards in Poland, located in the Forest District Susz. The obtained results indicated heterozygosity loss within the orchard, proving the existence of specimen selection effects on genetic variability. Hence, it seems quite important to take account of molecular genetic variability of maternal trees in future breeding strategies.

Low rates of pollen contamination in a Scots pine seed orchard in Sweden: the exception or the norm?

2015 ◽  
Vol 30 (7) ◽  
pp. 573-586 ◽  
Author(s):  
Tomas Funda ◽  
Ulfstand Wennström ◽  
Curt Almqvist ◽  
Takeshi Torimaru ◽  
Bengt Andersson Gull ◽  
...  
Keyword(s):  
Scots Pine ◽  
Seed Orchard ◽  
Pollen Contamination

The importance of being the first pollen in the strobili of Scots pine

2008 ◽  
Vol 38 (12) ◽  
pp. 2976-2980 ◽  
Author(s):  
Saila Varis ◽  
Arja Santanen ◽  
Anne Pakkanen ◽  
Pertti Pulkkinen
Keyword(s):  
Pinus Sylvestris ◽  
Scots Pine ◽  
Pollen Grains ◽  
Seed Orchard ◽  
Pollen Grain ◽  
Seed Orchards ◽  
Pollination Experiments ◽  
Seed Crop ◽  
Pollen Types ◽  
Pine Pollen

Timing of pollen arrival may affect the level of seeds fertilized by pollen from outside seed orchards, especially in seed orchards of Scots pine ( Pinus sylvestris L.) established in southern Finland with stock from northern populations. We performed sequential pollination experiments with Scots pine pollen collected from seed orchard clones originating from southern and northern Finland and recipient strobili in clones originating from southern Finland. When southern pollen was introduced first, seeds were sired equally by northern and southern genotypes. When northern pollen was introduced first, northern genotypes were found in 75% of the resulting seeds. When both pollen types were introduced at the same time, the resulting seed crop was split almost equally between northern and southern genotypes. These results do not unconditionally support the idea that the first pollen grain in the pollen chamber always fertilizes the ovum; instead, they suggest a more complex way of competition between pollen grains.

Reproductive success of orchard and nonorchard pollens during different stages of pollen shedding in a Scots pine seed orchard

1996 ◽  
Vol 26 (6) ◽  
pp. 1096-1102 ◽  
Author(s):  
Anni M. Harju ◽  
Teijo Nikkanen
Keyword(s):  
Reproductive Success ◽  
Scots Pine ◽  
Seed Orchard ◽  
Wind Pollination ◽  
Isozyme Markers ◽  
Seed Crop ◽  
Pollen Contamination ◽  
Pollination Season ◽  
Number Of Seeds

The reproductive success of orchard and nonorchard pollens was studied in seeds from megastrobili exposed during different stages of pollen shedding in a Pinussylvestris L. seed orchard. Wind pollination was controlled experimentally by isolating and then temporarily exposing the megastrobili to four different stages of pollen shedding by orchard trees. The amount of pollen contamination in the seed crop was determined using isozyme markers and an undetected gamete model. The estimated level of pollen contamination was 48% when megastrobili were exposed during the entire pollination season. Contamination was highest (60%) when megastrobili were exposed before actual pollen shedding by orchard trees, and lowest (27%) in seeds from megastrobili exposed during maximum pollen shedding by orchard trees. Reproductive success was measured several ways: as the total number of seeds produced in each treatment, as the ratio or proportion of mature cones per megastrobilus, and as the number of seeds produced per cone or megastrobilus. In 1991, reproductive success was highest before maximum pollen shedding by seed orchard trees. Pollen shedding by orchard trees occurred a few days after most megastrobili became receptive.

Reduction of pollen contamination in Scots pine seed orchard crop by tent isolation

2013 ◽  
Vol 28 (8) ◽  
pp. 715-723 ◽  
Author(s):  
Takeshi Torimaru ◽  
Ulfstand Wennström ◽  
Bengt Andersson ◽  
Curt Almqvist ◽  
Xiao-Ru Wang
Keyword(s):  
Scots Pine ◽  
Seed Orchard ◽  
Pollen Contamination

scholarly journals Identification of Pinus sylvestris clones with highest and lowest allelopathic potential

2019 ◽  
Vol 25 (1) ◽  
Author(s):  
Ernest Bielinis ◽  
Jacek Kwiatkowski ◽  
Sergii Boiko
Keyword(s):  
Pinus Sylvestris ◽  
Scots Pine ◽  
Seed Orchard ◽  
Inhibitory Effect ◽  
Test Plant ◽  
Allelopathic Potential ◽  
Clonal Seed Orchard ◽  
Wide Range ◽  
Germination And Growth ◽  
Distribution In Europe

Allelopathy is a kind of interaction between plants in which the inhibitory effect on growth and germination can play an important role during the struggle for existence in interspecific competition. The species (or clone of one species) with a higher allelopathic potential might win the competition and place themselves in a better biosocial position for growth. Also, the clones with a lower allopathic potential might be useful in agroforestry, e.g.  as trees useful for shading the crops. The allelopathic potential of Scots pine (Pinus sylvestris L.), the tree species with a wide range of distribution in Europe and of great economic importance, was estimated in this study. To this end, needles from trees growing in a clonal seed orchard were collected and used as a material to obtain water leachates. The leachates were diluted to 25 and 50% and used in allelopathy tests. The differences between potentials of leachates from Scots pine clones to inhibit germination and growth of the test plant (Sinapis alba L. cv. Borowska) were assessed. The clones 1702 and 1703 were characterized as highly allelopathic, with the potential confirmed in two independent allelopathic tests. The clones 355 and 2209 were characterized as clones with a moderate allopathic potential. The possibility of selecting clones with the known allelopathic potential for the successful use in forestry was discussed.

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