Barley (Hordeum vulgare L.): Anther Culture and the Production of Haploids

Author(s):  
Zhi-Hong Xu
Plant Science ◽  
2002 ◽  
Vol 162 (5) ◽  
pp. 843-847 ◽  
Author(s):  
G. Wojnarowiez ◽  
C. Jacquard ◽  
P. Devaux ◽  
R.S. Sangwan ◽  
C. Clément

2008 ◽  
Vol 22 (1) ◽  
pp. 119-129 ◽  
Author(s):  
M. Muñoz-Amatriaín ◽  
A. M. Castillo ◽  
X. W. Chen ◽  
L. Cistué ◽  
M. P. Vallés

1992 ◽  
Vol 43 (1) ◽  
pp. 67 ◽  
Author(s):  
DJ Luckett ◽  
RA Smithard

Agarose-solidified media containing maltose and glutamine were used to produce double-haploid lines, by anther culture, from Australian spring barley (Hordeum vulgare L.) genotypes. Three sets of F1 and two sets of F2 breeder's hybrids were used. Genotype differences were significant for the proportion of anthers responding, and the number of green and albino regenerants per 100 anthers plated. It was confirmed that the environmental conditions experienced by the donor plants (time of year, and field or glasshouse) had a large impact upon explant performance in culture. A minimum level of anther culture efficiency was achieved to allow a breeding program to produce all its inbred lines by this method. The procedure has the potential to save research costs, and deliver improved genotypes to the grower up to four years earlier than conventional methods.


1985 ◽  
Vol 65 (1) ◽  
pp. 29-32 ◽  
Author(s):  
J. M. YE ◽  
B. L. HARVEY ◽  
K. N. KAO

Anthers of two barley (Hordeum vulgare L.) cultivars, Bonanza and Elrose were cultured in media A or K1 with different levels of 2,4-D and zeatin riboside (Z-r). The results indicated that a combination of 2,4-D and Z-r in their optimal ranges (0.25–1.0 mg/L and 0.125–0.25 mg/L, respectively) significantly increased pollen callus induction in Bonanza anthers. Addition of 2,4-D alone (optimal range 0.25–2.0 mg/L) increased the frequency of callus formation in Elrose, while Z-r (0.125–2.0 mg/L) had no obvious effect on callus induction. When Z-r concentration was higher than 2.0 mg/L it decreased callus formation. At high concentrations (2.0–4.0 mg/L) 2,4-D alone induced loose calli which were not able to develop into plantlets.Key words: Barley, anther culture, 2,4-D, zeatin riboside


2017 ◽  
Vol 131 (2) ◽  
pp. 247-257 ◽  
Author(s):  
Katarzyna Makowska ◽  
Marta Kałużniak ◽  
Sylwia Oleszczuk ◽  
Janusz Zimny ◽  
Andrzej Czaplicki ◽  
...  

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