Doubled haploid production by anther culture for Australian barley breeding

1992 ◽  
Vol 43 (1) ◽  
pp. 67 ◽  
Author(s):  
DJ Luckett ◽  
RA Smithard
Keyword(s):  
Hordeum Vulgare ◽  
Anther Culture ◽  
Doubled Haploid ◽  
Environmental Conditions ◽  
Double Haploid ◽  
Spring Barley ◽  
Inbred Lines ◽  
Minimum Level ◽  
Hordeum Vulgare L ◽  
Time Of Year

Agarose-solidified media containing maltose and glutamine were used to produce double-haploid lines, by anther culture, from Australian spring barley (Hordeum vulgare L.) genotypes. Three sets of F1 and two sets of F2 breeder's hybrids were used. Genotype differences were significant for the proportion of anthers responding, and the number of green and albino regenerants per 100 anthers plated. It was confirmed that the environmental conditions experienced by the donor plants (time of year, and field or glasshouse) had a large impact upon explant performance in culture. A minimum level of anther culture efficiency was achieved to allow a breeding program to produce all its inbred lines by this method. The procedure has the potential to save research costs, and deliver improved genotypes to the grower up to four years earlier than conventional methods.

scholarly journals Structural Equation Model as a Tool to Assess the Relationship Between Grain Yield Per Plant and Yield Components in Doubled Haploid Spring Barley Lines (Hordeum vulgare L.)

2016 ◽  
Vol 73 (1) ◽  
pp. 63-77 ◽  
Author(s):  
Dariusz R. Mańkowski ◽  
Janusz Kozdój ◽  
Monika Janaszek-Mańkowska
Keyword(s):  
Hordeum Vulgare ◽  
Grain Yield ◽  
Doubled Haploid ◽  
Yield Components ◽  
Structural Equation ◽  
Spring Barley ◽  
Equation Model ◽  
Path Model ◽  
Equation Modeling ◽  
Hordeum Vulgare L

Abstract The aim of this study was to describe and characterize the relationships between yielding factors and grain yield per doubled haploid (DH) plant of spring barley as well as relation between yield components and duration of each stage of plant development. To describe these relations structure equation modeling was used. The study included plants of doubled haploid spring barley lines (Hordeum vulgare L.) derived from two-rowed form of Scarlett cultivar. The SAS® system was used to analyze the model of relationships between grain yield per plant and yield components. Our results indicate that the number of spikes per plant and grain yield per spike had a direct and decisive influence on the grain yield of the investigated DH plants of spring barley. Based on the path model analysis it was found that the most important factor determining grain yield per DH plants of spring barley was the number of spikes per plant and the duration of tillering and shooting stages.

Barley seed storage under controlled conditions

2019 ◽  
pp. 140-150 ◽  
Author(s):  
O. A. Zadorozhna ◽  
T. P. Shyianova ◽  
M.Yu. Skorokhodov
Keyword(s):  
Hordeum Vulgare ◽  
Moisture Content ◽  
Spring Barley ◽  
Seed Storage ◽  
Growing Season ◽  
Storage Conditions ◽  
Meteorological Conditions ◽  
Hordeum Vulgare L ◽  
The Relationship ◽  
And Storage

Seed longevity of 76 spring barley gene pool samples (Hordeum vulgare L. subsp. distichon, convar. distichon: 56 nutans Schubl., two deficience (Steud.) Koern., two erectum Rode ex Shuebl., two medicum Koern.; convar. nudum (L.) A.Trof.: one nudum L. та subsp. vulgare: convar. vulgare: nine pallidum Ser., three rikotense Regel.; convar. coeleste (L.) A.Trof.: one coeleste (L.) A.Trof.) from 26 countries, 11 years and four places of reproduction was analyzed. Seeds with 5–8% moisture content were stored in chamber with unregulated and 4oC temperature. The possibility of seed storage under these conditions for at least 10 years without significant changes in germination has been established. The importance of meteorological conditions in the formation and ripening of seeds for their longevity is confirmed. The relationship between the decrease of barley seeds longevity and storage conditions, amount of rainfall, temperature regime during the growing season of plants is discussed.

scholarly journals The Influence of Genotype and Environment on Arabinoxylan and Beta-glucan Contents in Grain of Spring Barley (Hordeum vulgare L.)

2014 ◽  
Vol 62 (3) ◽  
pp. 553-560 ◽  
Author(s):  
Pavel Macháň ◽  
Jaroslava Ehrenbergerová ◽  
Radim Cerkal ◽  
Karolína Benešová ◽  
Kateřina Vaculová
Keyword(s):  
Hordeum Vulgare ◽  
Spring Barley ◽  
Limiting Factors ◽  
Environment Interaction ◽  
Hordeum Vulgare L ◽  
Beta Glucan ◽  
Fungicide Treatment ◽  
Spring Variety ◽  
Beta Glucans ◽  
Growing Environment

Arabinoxylan and beta-glucan contents are limiting factors for a wider use of barley production. Arabinoxylan and beta-glucan contents were assessed in grain samples in sets of seven malting hulled varieties, three hull-less lines and one hull-less spring variety grown in the localities of Branišovice, Žabčice, and Kroměříž in 2009 to 2011. Further, the effect of growing technologies on the level of these non-starch polysaccharides was studied. Variability of arabinoxylan contents was affected most significantly by a genotype and growing technology whereas variability of beta-glucan contents was mostly affected by a genotype and growing environment (interaction of year with locality). The highest values of arabinoxylans and beta-glucans were determined in the grain samples of hull-less lines (KM 1057: 6.16% of arabinoxylans and KM 2084: 6.41% of beta-glucans) and on the contrary, the lowest values of arabinoxylans were found in the grain of hull-less variety AF Lucius (3.85%) and lowest amounts of beta-glucans were found in malting variety Radegast (3.92%). The samples of the growing technology without fungicide treatment had on average more arabinoxylans and beta-glucans than the fungicide non-treated ones.

COMPARAISON ENTRE DES LIGNEES PEDIGREES ET DES LIGNEES HAPLOIDES DOUBLEES CHEZ L’ORGE (HORDEUM VULGARE L.)

1980 ◽  
Vol 60 (1) ◽  
pp. 79-85 ◽  
Author(s):  
PIERRE TURCOTTE ◽  
C. A. ST-PIERRE ◽  
KEH MING HO
Keyword(s):  
Hordeum Vulgare ◽  
Doubled Haploid ◽  
Doubled Haploids ◽  
Block Design ◽  
Pure Line ◽  
Kernel Weight ◽  
Hordeum Vulgare L ◽  
Line Production ◽  
Doubled Haploid Lines ◽  
Time Required

Pedigree and doubled haploid lines from seven crosses of barley (Hordeum vulgare L.) were compared over 2 years. The lines were tested, in a randomized complete block design, in row plots at Ste-Foy in 1977 and in hill plots at Brawley, California in 1978. There are significant differences between the two methods of pure line production for grain yield, 1000-kernel weight, plant height, resistance to lodging and date of maturity. Furthermore, these significant differences between doubled haploid and pedigree lines seem to be tied to wide crosses. After showing that doubled haploids are superior for resistance to lodging, we conclude that the use of doubled haploid lines must be seriously considered in a barley breeding program on the basis of improved selection efficiency and the short time required to get homozygous lines.

Comparison of Selection Efficiency for Spring Barley (Hordeum vulgare L.) under Organic and Conventional Farming Conditions

Crop Science ◽  
2017 ◽  
Vol 57 (2) ◽  
pp. 626-636 ◽  
Author(s):  
Aina Kokare ◽  
Linda Legzdina ◽  
Chris Maliepaard ◽  
Rients E. Niks ◽  
Edith T. Lammerts van Bueren
Keyword(s):  
Hordeum Vulgare ◽  
Spring Barley ◽  
Conventional Farming ◽  
Selection Efficiency ◽  
Hordeum Vulgare L ◽  
Organic And Conventional Farming
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