Ultrastructure of Intermediate Lobe of the Pituitary and Melanocyte-Stimulating Hormone Secretion in Oestrogen-Induced Kidney Tumours in Male Hamsters

1979 ◽  
pp. 41-45 ◽  
Author(s):  
P. G. Saluja ◽  
J. M. Hamilton ◽  
A. J. Thody ◽  
A. A. Ismail ◽  
J. Knowles
Keyword(s):  
Hormone Secretion ◽  
Intermediate Lobe ◽  
Melanocyte Stimulating Hormone ◽  
Stimulating Hormone

DISTRIBUTION OF RADIOACTIVITY IN THE ORGANS OF THE RAT AND MOUSE AFTER INJECTION OF [125I]α-MELANOCYTE-STIMULATING HORMONE

1975 ◽  
Vol 64 (2) ◽  
pp. 237-241 ◽  
Author(s):  
A. DUPONT ◽  
A. J. KASTIN ◽  
F. LABRIE ◽  
G. PELLETIER ◽  
R. PUVIANI ◽  
...  
Keyword(s):  
Pineal Gland ◽  
Direct Measurement ◽  
Whole Body ◽  
Intermediate Lobe ◽  
High Uptake ◽  
Melanocyte Stimulating Hormone ◽  
Whole Body Autoradiography ◽  
Very High ◽  
Stimulating Hormone

SUMMARY The distribution of radioactivity after intrajugular injection of 125Ilabelled α-melanocyte-stimulating hormone (α-MSH) was studied by whole-body autoradiography of the mouse and by direct measurement of radioactivity in individual organs of the rat. Very high uptake of radioactivity in the pineal gland was measured 5 min after the injection of [125I]α-MSH. Lower levels of accumulation of radioactivity were found in the kidney and in the posterior (including intermediate) lobe of the pituitary. High uptake was also found in the thyroid, stomach, and oesophagus. The specificity of uptake of [125I] α-MSH into the pineal and pituitary is suggested by the very low uptake of Na125I into those tissues.

scholarly journals α-Melanocyte-stimulating-hormone precursors in the pig pituitary

1986 ◽  
Vol 235 (3) ◽  
pp. 715-722 ◽  
Author(s):  
M Fenger
Keyword(s):  
Adrenocorticotropic Hormone ◽  
Anterior Lobe ◽  
Intermediate Lobe ◽  
Reverse Phase ◽  
Melanocyte Stimulating Hormone ◽  
Pig Pituitaries ◽  
Stimulating Hormone

The occurrence of intermediates from the processing of ACTH-(1-39) [adrenocorticotropic hormone-(1-39)] to alpha-melanocyte-stimulating hormone was investigated in normal pig pituitaries by the use of sensitive and specific radioimmunoassays for ACTH-(1-13), ACTH-(1-14), ACTH-(1-13)-NH2 and ACTH-(1-39). Fractionation by reverse-phase h.p.l.c. revealed ACTH(1-17) and their acetylated analogues. The intermediate lobe contained NO-diacetyl-ACTH-(1-13)-NH2, N-acetyl-ACTH-(1-13)-NH2 and ACTH-(1-13)-NH2. In addition, the corresponding ACTH-(1-14) peptides (the glycine-extended precursor of the amidated peptides) were detected in lower amounts in both the intermediate lobe and the anterior lobe. ACTH-(1-17), ACTH-(1-13) and their acetylated analogues could not be detected in the anterior lobe or the intermediate lobe. The results suggest that an endopeptidase initially cleaves ACTH-(1-39) at the Lys-16-Arg-17 bond. ACTH-(1-16) is then processed by a pituitary carboxypeptidase to ACTH-(1-14) and ACTH-(17-39) by the aminopeptidase to ACTH-(18-39).

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