Adipose Conversion of Murine Bone Marrow-Derived Macrophages in Serum-Free Medium

1983 ◽  
pp. 304-306 ◽  
Author(s):  
Inge Flesch ◽  
Uwe-Peter Ketelsen ◽  
Ernst Ferber
Keyword(s):  
Bone Marrow ◽  
Serum Free Medium ◽  
Free Medium ◽  
Serum Free ◽  
Murine Bone Marrow ◽  
Adipose Conversion

Therapeutic Impact of Human Bone Marrow Stromal Cells Expanded by Animal Serum–Free Medium for Cerebral Infarct in Rats

Neurosurgery ◽  
2011 ◽  
Vol 68 (6) ◽  
pp. 1733-1742 ◽  
Author(s):  
Taku Sugiyama ◽  
Satoshi Kuroda ◽  
Yukari Takeda ◽  
Mitsufumi Nishio ◽  
Masaki Ito ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Stromal Cells ◽  
Bone Marrow Stromal Cells ◽  
Marrow Stromal Cells ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Serum Free Medium ◽  
Free Medium ◽  
Serum Free ◽  
Therapeutic Impact

Human liquid bone marrow culture in serum-free medium

1989 ◽  
Vol 73 (2) ◽  
pp. 143-147 ◽  
Author(s):  
Xavier Drouet ◽  
Luc Douay ◽  
Marie-Catherine Giarratana ◽  
Claude Baillou ◽  
Norbert-Claude Gorin ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Bone Marrow Culture ◽  
Serum Free Medium ◽  
Free Medium ◽  
Serum Free

Megakaryocytes Cultured from Patients with IMF Produced Significantly Higher mRNA but Not Protein Levels of Fibrosing Grown Factors.

Blood ◽  
2006 ◽  
Vol 108 (11) ◽  
pp. 3607-3607
Author(s):  
Jen-Chin Wang ◽  
Tsong H Chang ◽  
Amit Goldberg ◽  
Allan D. Novetsky ◽  
Steven Lichter ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Growth Factors ◽  
Growth Factor ◽  
Mrna Levels ◽  
Serum Free Medium ◽  
Free Medium ◽  
Myeloid Metaplasia ◽  
Serum Free ◽  
Protein Levels

Abstract Currently, the prevailing concept concerning the etiology of bone marrow fibrosis in patients with idiopathic myelofibrosis (IMF) is that it results from excessive production of fibrosing growth factors including transforming growth factor beta (TGF-B1), platelet-derived growth factor (PDGF), and fibroblast growth factor (FGF) from megakaryocytes and monocytes. Since megakaryocytes are difficult to isolate from bone marrow in IMF patients, this concept remains speculative. We obtained megakaryocytes (CD41+ cells) from 10-day in vitro culture of blood CD34+ cells in serum-free medium with thrombopoietin and stem cell factors as described and cultured monocytes from isolating blood CD14+ cells. Then quantitative analyses of fibrosing growth factors at the mRNA and protein levels were obtained. mRNA levels were obtained from real-time RT-PCR technique, and protein levels were obtained from ELISA analysis of the supernatant of CD41+ cells cultured 4 h in serum-free medium. The results showed 1) mRNA levels of TGF-B1, PDGF, and FGF produced by the megakaryocytes were significantly elevated in agnogenic myeloid metaplasia (AMM) compared with those in normal controls (p<0.05). While these growth factors were elevated several-fold in AMM compared with other myeloproliferative disorders (MPD) including essential thrombocythemia and polycythemia vera, they were not statistically significant. 2) mRNA levels of TGF-B1 were higher than levels of PDGF or FGF. 3) The mRNA levels of these growth factors produced from CD14+ cells were not significantly elevated in AMM compared with other MPDs or controls; the AMM mRNA levels were significantly elevated only in some patients. 4) The correlation of mRNA levels of these growth factors with the degree of myelofibrosis in AMM was significant with megakaryocytes (r=0.73) but not with monocytes (r=0.23). 5) ELISA analysis of the growth factors from the cultured megakaryocytes showed that, in most of the patients with AMM and other MPDs and in volunteer controls, the growth factors were undetectable, and only a few patients with AMM had significantly elevated protein levels of these growth factors. We conclude thatin IMF, megakaryocytes but not monocytes are the predominant cells producing fibrosing growth factors, andthe failure of finding increased protein levels of these growth factors in the in vitro system suggest that other factors are necessary to initiate translation of these growth factors in the megakaryoctes, and neutrophil emperipolesis with releasing factors may be important in this process.

Expansion, characterization, and differentiation of rabbit bone marrow-derived mesenchymal stem cells in serum-free medium

2014 ◽  
Vol 18 (4) ◽  
pp. 228-236 ◽  
Author(s):  
Lifang Jin ◽  
Shaohui Ji ◽  
Mei Shen ◽  
Jianlong Zhang ◽  
Jiwei Han ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Serum Free Medium ◽  
Free Medium ◽  
Serum Free ◽  
Rabbit Bone

scholarly journals Bone Marrow Mesenchymal Stem Cells Enhance the Differentiation of Human Switched Memory B Lymphocytes into Plasma Cells in Serum-Free Medium

2016 ◽  
Vol 2016 ◽  
pp. 1-18 ◽  
Author(s):  
Guillaume Bonnaure ◽  
Catherine Gervais-St-Amour ◽  
Sonia Néron
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
B Lymphocytes ◽  
Plasma Cells ◽  
Serum Free Medium ◽  
Free Medium ◽  
Low Oxygen ◽  
Serum Free ◽  
Marrow Mesenchymal Stem Cells

The differentiation of human B lymphocytes into plasma cells is one of the most stirring questions with regard to adaptive immunity. However, the terminal differentiation and survival of plasma cells are still topics with much to be discovered, especially when targeting switched memory B lymphocytes. Plasma cells can migrate to the bone marrow in response to a CXCL12 gradient and survive for several years while secreting antibodies. In this study, we aimed to get closer to niches favoring plasma cell survival. We tested low oxygen concentrations and coculture with mesenchymal stem cells (MSC) from human bone marrow. Besides, all cultures were performed using an animal protein-free medium. Overall, our model enables the generation of high proportions of CD38+CD138+CD31+plasma cells (≥50%) when CD40-activated switched memory B lymphocytes were cultured in direct contact with mesenchymal stem cells. In these cultures, the secretion of CXCL12 and TGF-β, usually found in the bone marrow, was linked to the presence of MSC. The level of oxygen appeared less impactful than the contact with MSC. This study shows for the first time that expanded switched memory B lymphocytes can be differentiated into plasma cells using exclusively a serum-free medium.

The role of recombinant haematopoietic growth factors in human long-term bone marrow culture in serum-free medium

1991 ◽  
Vol 79 (1) ◽  
pp. 27-32 ◽  
Author(s):  
Luc Douay ◽  
Marie-Catherine Giarratana ◽  
Xavier Drouet ◽  
Dominique Bardinet ◽  
Norbert-Claude Gorin
Keyword(s):  
Bone Marrow ◽  
Growth Factors ◽  
Bone Marrow Culture ◽  
Serum Free Medium ◽  
Free Medium ◽  
Serum Free
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