Induction of Enzymes of Phytoalexin Synthesis in Soybean Cells by Fungal Elicitor

1985 ◽  
pp. 247-254 ◽  
Author(s):  
J. Ebel ◽  
M. R. Stäb ◽  
W. E. Schmidt
Keyword(s):  
Fungal Elicitor ◽  
Induction Of Enzymes ◽  
Phytoalexin Synthesis

Further Studies on Induction of Enzymes of Phytoalexin Synthesis in Soybean and Cultured Soybean Cells

1983 ◽  
Vol 38 (9-10) ◽  
pp. 730-735 ◽  
Author(s):  
Joachim Leube ◽  
Hans Grisebach
Keyword(s):  
Cell Cultures ◽  
Reductase Activity ◽  
Phytophthora Megasperma ◽  
Hmg Coa Reductase ◽  
Race 1 ◽  
Induction Of Enzymes ◽  
Elicitor Treatment ◽  
Coa Reductase ◽  
Soybean Cell ◽  
Phytoalexin Synthesis

Abstract The glucan elicitor from cell walls o f the fungal pathogen, Phytophthora megasperma f. sp. glycinea, caused a decrease in activity of 3-hydroxy-3-methylglutaryl coenzyme A reductase in wounded soybean cotyledons, whereas wounding alone led to an increase in the activity of this enzyme. A decrease o f HMG -CoA reductase activity after elicitor treatment was also found in soybean hypocotyls and soybean cell cultures. In contrast to the activity o f HMG -CoA reductase, the activity of dimethylallylpyrophosphate: 3,6a,9-trihydroxypterocarpan dimethylallyltransferase increases after elicitor challenge of soy­bean cell cultures and after inoculation of soybean hypocotyls (cv Amsoy 71) with mycelium of either race 1 (incompatible) or race 3 (compatible) of P. megasperma.

Temperature-induced alanine oxidation in a psychrotrophic Pseudomonas

1975 ◽  
Vol 21 (12) ◽  
pp. 2028-2033
Author(s):  
Prince K. Zachariah ◽  
John Liston
Keyword(s):  
Heat Treatment ◽  
Enzyme Synthesis ◽  
Optimum Temperature ◽  
Temperature Optimum ◽  
Ph Optimum ◽  
Sephadex Column ◽  
Oxidase System ◽  
Psychrotrophic Bacteria ◽  
Induction Of Enzymes ◽  
Elution Fraction

A psychrotrophic pseudomonad isolated from iced fish oxidized alanine at temperatures close to 0 °C and grew over the range 0 °C–35 °C. The rate of oxidation of alanine, measured manometrically, by cells grown at 2 °C was lower than that of cells grown at 22 °C. However, the consumption of oxygen after heat treatment at 35 °C for 35 min was reduced considerably by 2 °C grown cells. Alanine oxidase activity was tested in an extract from cells grown at 2 °C and 22 °C with alanine as the sole carbon, nitrogen, and energy source. Cells grown at 2 °C produced an alanine oxidase with a temperature optimum of 35 °C and pH optimum of 8, which lost about 80% activity by heat treatment at 40 °C for 30 min. There was no change in activity after dialysis at pH 7, 8, or 9. Extracts from cells grown at 22 °C contained an alanine oxidase system with an optimum temperature of 45 °C, a pH optimum above 8, and only about 30% reduction of activity after heat treatment. This enzyme activity was concentrated in the 0.5 M elution fraction from a Sephadex column, and dialysis reduced the activity at pH 7 and 8. Mesophilic enzyme synthesis apparently started around a growth temperature of 10 °C.The crude alanine oxidase systems of Pseudomonas aeruginosa derived from cells grown at 13 °C and 37 °C had a common optimum temperature of 45 °C. These data suggest that one mechanism of psychrophilic growth by psychrotrophic bacteria may be the induction of enzymes with low optimum temperatures in response to low temperature conditions.

Characterization of binding proteins that recognize oligoglucoside elicitors of phytoalexin synthesis in soybean

1995 ◽  
Vol 93 (2) ◽  
pp. 401-410 ◽  
Author(s):  
Francois Cote ◽  
Jong-Joo Cheong ◽  
Rob Alba ◽  
Michael G. Hahn
Keyword(s):  
Binding Proteins ◽  
Phytoalexin Synthesis
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