Surgical Techniques for Mouse Heart Transplantation

1998 ◽  
pp. 185-192
Author(s):  
Z. K. Chen ◽  
S. Metcalfe
Keyword(s):  
Heart Transplantation ◽  
Surgical Techniques ◽  
Mouse Heart

Blood Circuit Reconstruction in an Abdominal Mouse Heart Transplantation Model

10.3791/62007 ◽  
2021 ◽  
Author(s):  
Decheng Yin ◽  
Jian Fu ◽  
Ida Allabauer ◽  
Oliver Witzke ◽  
Song Rong ◽  
...  
Keyword(s):  
Heart Transplantation ◽  
Mouse Heart ◽  
Transplantation Model

Current status of heart transplantation

2018 ◽  
pp. 223-240
Author(s):  
Ayyaz Ali ◽  
Robert L. Kormos
Keyword(s):  
Heart Transplantation ◽  
Cardiac Transplantation ◽  
Organ Procurement ◽  
Severe Heart Failure ◽  
Surgical Techniques ◽  
Current Status ◽  
Definitive Treatment ◽  
Term Survival ◽  
The Many ◽  
End Stage

Cardiac transplantation has extended and improved the lives of patients suffering from severe heart failure over many decades. Despite advances in medical therapy, cardiac transplantation remains the definitive treatment for end-stage heart disease. Surgical techniques for organ procurement and implantation, development of appropriate methods for preserving the heart, and understanding the immunological challenges associated with transplantation were among the many areas which required focused investigation. In the current era, heart transplantation is associated with a low operative mortality and excellent long-term survival, however, the major obstacle of shortage of suitable donor organs remains. In the following chapter, recipient selection and management, donor organ procurement and preservation, and surgical techniques of heart transplantation are described in detail.

scholarly journals Reduction of Cold Ischemic Injury with the Addition of Compound Glycyrrhizin in HTK Solution in a Mouse Heart Transplantation Model

2020 ◽  
Vol 61 (3) ◽  
pp. 595-600
Author(s):  
Jiali Wu ◽  
Xiangdong Liu ◽  
Maohua Wang ◽  
Xiaobin Wang ◽  
De Luo ◽  
...  
Keyword(s):  
Heart Transplantation ◽  
Ischemic Injury ◽  
Mouse Heart ◽  
Htk Solution ◽  
Transplantation Model

Novel technique for blood circuit reconstruction in mouse heart transplantation model

Microsurgery ◽  
2006 ◽  
Vol 26 (8) ◽  
pp. 594-598 ◽  
Author(s):  
Kun Wu ◽  
Jinyan Zhang ◽  
Jian Fu ◽  
Shengli Wu ◽  
Thomas Philipp ◽  
...  
Keyword(s):  
Heart Transplantation ◽  
Mouse Heart ◽  
Novel Technique ◽  
Transplantation Model

Paediatric heart transplantation: an update

2019 ◽  
Vol 104 (12) ◽  
pp. 1216-1222
Author(s):  
Zdenka Reinhardt
Keyword(s):  
Heart Transplantation ◽  
Organ Procurement ◽  
Surgical Techniques ◽  
Immunosuppressive Drugs ◽  
Lymphoproliferative Disease ◽  
Current Status ◽  
Term Survival ◽  
Post Transplant ◽  
Monitoring Strategies ◽  
End Stage

Heart transplantation is a standard treatment for selected paediatric patients with end-stage heart disease. With improvement in surgical techniques, organ procurement and preservation strategies, immunosuppressive drugs, and more sophisticated monitoring strategies, survival following transplantation has increased over time. However, rejection, infection, renal failure, post-transplant lymphoproliferative disease and post-transplant cardiac allograft vasculopathy still preclude long-term survival. Therefore, continued multidisciplinary scientific efforts are needed for future gains. This review focuses on the current status, outcomes and ongoing challenges including patient selection, indications and contraindications, national and international survivals, post-transplant complications and quality of life.

Abstract 137: Ctcf is Essential for Transcriptome Regulation in the Healthy Heart

2016 ◽  
Vol 119 (suppl_1) ◽  
Author(s):  
Manuel Rosa-Garrido ◽  
Todd Kimball ◽  
Douglas J Chapski ◽  
Tsai-Ting Shih ◽  
Elizabeth Soehalim ◽  
...  
Keyword(s):  
Gene Expression ◽  
Heart Failure ◽  
Severe Heart Failure ◽  
Surgical Techniques ◽  
Ctcf Binding ◽  
Global Changes ◽  
Mouse Heart ◽  
Precise Control ◽  
Transcriptome Regulation ◽  
Heart Size

Heart failure is a syndrome resulting from complex genetic predisposition and multiple environmental factors. It is well established that global changes in gene expression accompany the transition through hypertrophy and on to overt failure producing deterioration of function at the organ level. Unknown, however, are the global changes in chromatin structure that allows for pathological gene expression. The epigenetic factor CTCF is a ubiquitous protein thought to be involved in maintenance of chromatin microenvironments. We found that hypertrophic stimulation down-regulates CTCF in the heart, and CTCF expression is significantly correlated with heart size in disease across genetic backgrounds. To test this relationship further, we have generated an inducible cardiac-specific CTCF knockout animal. These animals quickly develop severe heart failure characterized by a dilated left ventricle, decreased ejection fraction, and reactivation of the fetal gene program. Our analysis of published CTCF ChIP-seq data performed in mouse heart has also shown CTCF binding peaks flanking the fetal genes. Furthermore, we have found that CTCF abundance at these sites changes after hypertrophic agonist isoproterenol treatment, suggesting that CTCF protects from the development of cardiac disease by regulating gene expression. We also used RNA-seq to compare the transcriptome of adult myocytes isolated from control, CTCF KO mice, and mice that underwent transverse aortic constriction (TAC) to induce heart failure. These analyses showed that CTCF depletion remodels gene expression in a manner that mimics aspects of the pathological transcriptome in heart failure. Our data demonstrate that precise control of CTCF levels is required for normal transcriptome regulation in the adult heart and that alterations in CTCF levels (either via genetic, pharmacologic or surgical techniques) lead to cardiac pathology.

Heart Transplantation

2015 ◽  
pp. 756-779
Author(s):  
Ahmed El-Eshmawi ◽  
Anelechi Anyanwu
Keyword(s):  
Postoperative Complications ◽  
Heart Transplantation ◽  
Gold Standard ◽  
Standard Therapy ◽  
Standard Care ◽  
Surgical Techniques ◽  
Orthotopic Heart Transplantation ◽  
Infection Prophylaxis ◽  
Experimental Intervention ◽  
End Stage

Orthotopic heart transplantation (OHT) has evolved as the “gold standard” therapy for end stage cardiomyopathy, Advances in the fields of immunosuppression, infection prophylaxis and treatment, surgical techniques as well as intensice care management have transformed heart transplantation from what was once considered an experimental intervention into a standard therapy. This chapter focuses on the standard care for OHT including surgical techniques, perioperative management and management of common postoperative complications.

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