Establishment of Cell Lines of Vector Insects, and Infection of These Cell Monolayers with Rice Dwarf Virus (RDV)

Author(s):  
I. Kimura
Virology ◽  
1966 ◽  
Vol 28 (4) ◽  
pp. 571-579 ◽  
Author(s):  
Kin-Ichiro Miura ◽  
Ikuo Kimura ◽  
Naoji Suzuki

1989 ◽  
Vol 70 (5) ◽  
pp. 1297-1300 ◽  
Author(s):  
I. Uyeda ◽  
H. Kudo ◽  
T. Takahashi ◽  
T. Sano ◽  
K. Ohshima ◽  
...  

2001 ◽  
Vol 281 (2) ◽  
pp. C388-C397 ◽  
Author(s):  
C. J. Watson ◽  
M. Rowland ◽  
G. Warhurst

Despite significant advances in the characterization of tight junction (TJ) proteins, little is known about how molecular changes relate to function due primarily to the limitations of conventional paracellular probes. To address this, the paracellular pathway in Caco-2 and T84 cell lines was profiled by measuring the permeabilities of 24 polyethylene glycols (PEG) of increasing molecular radius (3.5–7.4 Å) analyzed by mass spectrometry. When combined with a paracellular sieving model, these data provided quantitative descriptors of the pathway under control conditions and after exposure to TJ modulators. PEG profiles in both cell lines conformed to a biphasic process involving a restrictive pore (radius 4.3–4.5 Å) and a nonrestrictive component responsible for permeability of larger molecules. PEG profiling revealed significant differences between the effects of EGTA and sodium caprate (C10). The restrictive component of EGTA-treated cells lost all size discrimination due to an increase in pore radius. Sodium caprate had no effect on pore radius but increased permeability via a different mechanism possibly involving increased numbers of functional pores. PEG profiling provides a useful tool for probing the functional regulation of the paracellular route.


2012 ◽  
Vol 46 (1) ◽  
pp. 65-71
Author(s):  
Hongyan CHEN ◽  
Taiyun WEI ◽  
Ai SAOTOME ◽  
Kengo SAKAGUCHI ◽  
Yasuo SHIKAMOTO ◽  
...  

Intervirology ◽  
1994 ◽  
Vol 37 (1) ◽  
pp. 6-11 ◽  
Author(s):  
Ichiro Uyeda ◽  
Narushi Suda ◽  
Naoki Yamada ◽  
Hiroshi Kudo ◽  
Kazunori Murao ◽  
...  

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