scholarly journals Endomembranes and myosin mediate assembly into tubules of Pns10 of Rice dwarf virus and intercellular spreading of the virus in cultured insect vector cells

Virology ◽  
2008 ◽  
Vol 372 (2) ◽  
pp. 349-356 ◽  
Author(s):  
Taiyun Wei ◽  
Takumi Shimizu ◽  
Toshihiro Omura
Keyword(s):  
Dwarf Virus ◽  
Insect Vector ◽  
Rice Dwarf Virus

Nonstructural protein Pns12 of rice dwarf virus is a principal regulator for viral replication and infection in its insect vector

2015 ◽  
Vol 210 ◽  
pp. 54-61 ◽  
Author(s):  
Qian Chen ◽  
Hongyan Chen ◽  
Dongsheng Jia ◽  
Qianzhuo Mao ◽  
Lianhui Xei ◽  
...  
Keyword(s):  
Viral Replication ◽  
Nonstructural Protein ◽  
Dwarf Virus ◽  
Insect Vector ◽  
Rice Dwarf Virus

scholarly journals A Novel Iflavirus Was Discovered in Green Rice Leafhopper Nephotettix cincticeps and Its Proliferation Was Inhibited by Infection of Rice Dwarf Virus

2021 ◽  
Vol 11 ◽  
Author(s):  
Wenxi Jia ◽  
Fei Wang ◽  
Jingjing Li ◽  
Xuefei Chang ◽  
Yi Yang ◽  
...  
Keyword(s):  
Infection Rate ◽  
Rna Virus ◽  
Antagonistic Effect ◽  
Dwarf Virus ◽  
Insect Vector ◽  
Rice Dwarf Virus ◽  
Reading Frame ◽  
Green Rice Leafhopper ◽  
Laboratory Populations ◽  
Dwarf Disease

The green rice leafhopper, Nephotettix cincticeps (Hemiptera: Cicadellidae), is a key insect vector transmitting rice dwarf virus (RDV) that causes rice dwarf disease. We discovered a novel iflavirus from the transcriptomes of N. cincticeps and named it as Nephotettix cincticeps positive-stranded RNA virus-1 (NcPSRV-1). The viral genome consists of 10,524 nucleotides excluding the poly(A) tail and contains one predicted open reading frame encoding a polyprotein of 3,192 amino acids, flanked by 5' and 3' untranslated regions. NcPSRV-1 has a typical iflavirus genome arrangement and is clustered with the members of the family Iflaviridae in the phylogenetic analysis. NcPSRV-1 was detected in all tested tissues and life stages of N. cincticeps and could be transmitted horizontally and vertically. Moreover, NcPSRV-1 had high prevalence in the laboratory populations and was widely spread in field populations of N. cincticeps. NcPSRV-1 could also infect the two-striped leafhopper, Nephotettix apicalis, at a 3.33% infection rate, but was absent in the zigzag leafhopper, Recilia dorsalis, and rice Oryza sativa variety TN1. The infection of RDV altered the viral load and infection rate of NcPSRV-1 in N. cincticeps, for which it seems that RDV has an antagonistic effect on NcPSRV-1 infection in the host.

scholarly journals Nonstructural protein Pns4 of rice dwarf virus is essential for viral infection in its insect vector

2015 ◽  
Vol 12 (1) ◽  
Author(s):  
Qian Chen ◽  
Linghua Zhang ◽  
Hongyan Chen ◽  
Lianhui Xie ◽  
Taiyun Wei
Keyword(s):  
Viral Infection ◽  
Nonstructural Protein ◽  
Dwarf Virus ◽  
Insect Vector ◽  
Rice Dwarf Virus

scholarly journals The Spread of Rice Dwarf Virus among Cells of Its Insect Vector Exploits Virus-Induced Tubular Structures

2006 ◽  
Vol 80 (17) ◽  
pp. 8593-8602 ◽  
Author(s):  
Taiyun Wei ◽  
Akira Kikuchi ◽  
Yusuke Moriyasu ◽  
Nobuhiro Suzuki ◽  
Takumi Shimizu ◽  
...  
Keyword(s):  
Viral Protein ◽  
Neutralizing Antibodies ◽  
Specific Binding ◽  
Host Cells ◽  
Dwarf Virus ◽  
Insect Vector ◽  
Rice Dwarf Virus ◽  
Tubular Structures ◽  
Intercellular Spread

ABSTRACT Various cytopathological structures, known as inclusion bodies, are formed upon infection of cultured leafhopper cells by Rice dwarf virus, a member of the family Reoviridae. These structures include tubules of approximately 85 nm in diameter which are composed of the nonstructural viral protein Pns10 and contain viral particles. Such tubular structures were produced in heterologous non-host insect cells that expressed Pns10 of the virus. These tubules, when associated with actin-based filopodia, were able to protrude from the surface of cells and to penetrate neighboring cells. A binding assay in vitro revealed the specific binding of Pns10 to actin. Infection of clusters of cells was readily apparent 5 days after inoculation at a low multiplicity of infection with the virus, even in the presence of neutralizing antibodies. However, treatment of host cells with drugs that inhibited the elongation of actin filaments abolished the extension of Pns10 tubules from the surface of cells, with a significant simultaneous decrease in the extent of infection of neighboring cells. These results together revealed a previously undescribed aspect of the intercellular spread of Rice dwarf virus, wherein the virus exploits tubules composed of a nonstructural viral protein and actin-based filopodia to move into neighboring cells.

Localization of rice dwarf virus in its insect vector

Virology ◽  
1963 ◽  
Vol 21 (3) ◽  
pp. 503-505 ◽  
Author(s):  
T. Fukushi ◽  
E. Shikata
Keyword(s):  
Dwarf Virus ◽  
Insect Vector ◽  
Rice Dwarf Virus

scholarly journals Entry of Rice Dwarf Virus into Cultured Cells of Its Insect Vector Involves Clathrin-Mediated Endocytosis

2007 ◽  
Vol 81 (14) ◽  
pp. 7811-7815 ◽  
Author(s):  
Taiyun Wei ◽  
Hongyan Chen ◽  
Tamaki Ichiki-Uehara ◽  
Hiroyuki Hibino ◽  
Toshihiro Omura
Keyword(s):  
Electron Microscopy ◽  
Cultured Cells ◽  
Negligible Effect ◽  
Immunofluorescence Staining ◽  
Dwarf Virus ◽  
Insect Vector ◽  
Rice Dwarf Virus ◽  
Bafilomycin A1 ◽  
Coated Pits ◽  
Early Endosomes

ABSTRACT Electron microscopy revealed that the entry of Rice dwarf virus (RDV) into insect vector cells involved endocytosis via coated pits. The treatment of cells with drugs that block receptor-mediated or clathrin-mediated endocytosis significantly reduced RDV infectivity. However, the drug that blocks caveola-mediated endocytosis had a negligible effect on such infection. Infection was also inhibited when cells had been pretreated with bafilomycin A1, which interferes with acidification of endosomes. Moreover, immunofluorescence staining indicated that the virus is internalized into early endosomes. Together, our data indicate that RDV enters insect vector cells through receptor-mediated, clathrin-dependent endocytosis and is sequestered in early endosomes.

scholarly journals Rice dwarf virus is engulfed into and released via vesicular compartments in cultured insect vector cells

2008 ◽  
Vol 89 (11) ◽  
pp. 2915-2920 ◽  
Author(s):  
Taiyun Wei ◽  
Hiroyuki Hibino ◽  
Toshihiro Omura
Keyword(s):  
Brefeldin A ◽  
Virus Multiplication ◽  
Dwarf Virus ◽  
Insect Vector ◽  
Rice Dwarf Virus ◽  
Transmission Electron ◽  
Viral Particles ◽  
Infected Cells ◽  
Vector Insect ◽  
Butanedione Monoxime

Vector insect cells infected with Rice dwarf virus had vesicular compartments containing viral particles located adjacent to the viroplasm when examined by transmission electron and confocal microscopy. Such compartments were often at the periphery of infected cells. Inhibitors of vesicular transport, brefeldin A and monensin, and an inhibitor of myosin motor activity, butanedione monoxime, abolished the formation of such vesicles and prevented the release of viral particles from infected cells without significant effects on virus multiplication. Furthermore, the actin-depolymerizing drug, cytochalasin D, inhibited the formation of actin filaments without significantly interfering with formation of vesicular compartments and the release of viruses from treated cells. These results together revealed intracellular vesicular compartments as a mode for viral transport in and release from insect vector cells infected with a plant-infecting reovirus.

Sequential infection of Rice dwarf virus in the internal organs of its insect vector after ingestion of virus

2011 ◽  
Vol 160 (1-2) ◽  
pp. 389-394 ◽  
Author(s):  
Hongyan Chen ◽  
Qian Chen ◽  
Toshihiro Omura ◽  
Tamaki Uehara-Ichiki ◽  
Taiyun Wei
Keyword(s):  
Dwarf Virus ◽  
Insect Vector ◽  
Rice Dwarf Virus ◽  
Internal Organs ◽  
Sequential Infection
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