The ras Oncogene Protein

1989 ◽  
pp. 85-91
Author(s):  
M. S. Marshall ◽  
M. D. Schaber ◽  
U. S. Vogel ◽  
W. S. Hill ◽  
A. S. Ng ◽  
...  
Keyword(s):  
Ras Oncogene ◽  
Oncogene Protein

Role of phosphatidylinositide metabolism in ras-induced Xenopus oocyte maturation

1990 ◽  
Vol 10 (3) ◽  
pp. 923-929
Author(s):  
B T Pan ◽  
G M Cooper
Keyword(s):  
Xenopus Oocytes ◽  
Second Messengers ◽  
Phospholipid Metabolism ◽  
Meiotic Maturation ◽  
Ras Oncogene ◽  
Ras Protein ◽  
32P Incorporation ◽  
Kinetics Of ◽  
Oncogene Protein

Microinjection of Xenopus oocytes with ras protein (p21) was used to investigate the role of phospholipid metabolism in ras-induced meiotic maturation. Induction of meiosis by ras was compared with induction by progesterone, insulin, and the phorbol ester 12-O-tetradecanoylphorbol-13-acetate (TPA). Neomycin, which specifically binds to phosphatidylinositides and inhibits their metabolism, blocked meiotic maturation induced by ras or insulin but not by progesterone or TPA. In addition, p21 and TPA, but not insulin or progesterone, stimulated the incorporation of 32Pi into oocyte lipids. ras protein specifically stimulated 32P incorporation into phosphatidylinositides, whereas both ras and TPA stimulated 32P incorporation into phosphatidylcholine and phosphatidylethanolamine. The stimulatory effect of p21 on phosphatidylinositide metabolism correlated with the dose response and kinetics of ras-induced meiotic maturation. In addition, the ras oncogene protein was more potent than the proto-oncogene protein both in inducing meiotic maturation and in stimulating phosphatidylinositide metabolism. These results indicate that phosphatidylinositide turnover is required for ras-induced meiosis and suggest that phosphatidylinositide-derived second messengers mediate the biological activity of ras in Xenopus oocytes.

scholarly journals Structure, signaling and the drug discovery of the Ras oncogene protein

BMB Reports ◽  
2017 ◽  
Vol 50 (7) ◽  
pp. 355-360 ◽  
Author(s):  
Chang Woo Han ◽  
Mi Suk Jeong ◽  
Se Bok Jang
Keyword(s):  
Drug Discovery ◽  
Ras Oncogene ◽  
Oncogene Protein

Structural differences between a ras oncogene protein and the normal protein

Nature ◽  
1989 ◽  
Vol 337 (6202) ◽  
pp. 90-93 ◽  
Author(s):  
Liang Tong ◽  
Abraham M. de Vos ◽  
Michael V. Milburn ◽  
Jarmila Jancarik ◽  
Shigeru Noguchi ◽  
...  
Keyword(s):  
Ras Oncogene ◽  
Structural Differences ◽  
Oncogene Protein

scholarly journals Equilibria between conformational states of the Ras oncogene protein revealed by high pressure crystallography

2022 ◽  
Author(s):  
Eric Girard ◽  
Rui P Lopes ◽  
Michael Spoerner ◽  
Anne-Claire Dhaussy ◽  
Thierry Prangé ◽  
...  
Keyword(s):  
High Pressure ◽  
Small Gtpase ◽  
Ras Oncogene ◽  
Conformational States ◽  
Ras Protein ◽  
Allosteric Transitions ◽  
High Pressure Crystallography ◽  
Oncogene Protein

In this work, we experimentally investigate the allosteric transitions between conformational states on the Ras oncogene protein using high pressure crystallography. Ras protein is a small GTPase involved in central...

Microinjection of the ras oncogene protein into PC12 cells induces morphological differentiation

Cell ◽  
1985 ◽  
Vol 42 (3) ◽  
pp. 841-848 ◽  
Author(s):  
Dafna Bar-Sagi ◽  
James R. Feramisco
Keyword(s):  
Pc12 Cells ◽  
Morphological Differentiation ◽  
Ras Oncogene ◽  
Oncogene Protein

scholarly journals Role of phosphatidylinositide metabolism in ras-induced Xenopus oocyte maturation.

1990 ◽  
Vol 10 (3) ◽  
pp. 923-929 ◽  
Author(s):  
B T Pan ◽  
G M Cooper
Keyword(s):  
Xenopus Oocytes ◽  
Second Messengers ◽  
Phospholipid Metabolism ◽  
Meiotic Maturation ◽  
Ras Oncogene ◽  
Ras Protein ◽  
32P Incorporation ◽  
Kinetics Of ◽  
Oncogene Protein

Microinjection of Xenopus oocytes with ras protein (p21) was used to investigate the role of phospholipid metabolism in ras-induced meiotic maturation. Induction of meiosis by ras was compared with induction by progesterone, insulin, and the phorbol ester 12-O-tetradecanoylphorbol-13-acetate (TPA). Neomycin, which specifically binds to phosphatidylinositides and inhibits their metabolism, blocked meiotic maturation induced by ras or insulin but not by progesterone or TPA. In addition, p21 and TPA, but not insulin or progesterone, stimulated the incorporation of 32Pi into oocyte lipids. ras protein specifically stimulated 32P incorporation into phosphatidylinositides, whereas both ras and TPA stimulated 32P incorporation into phosphatidylcholine and phosphatidylethanolamine. The stimulatory effect of p21 on phosphatidylinositide metabolism correlated with the dose response and kinetics of ras-induced meiotic maturation. In addition, the ras oncogene protein was more potent than the proto-oncogene protein both in inducing meiotic maturation and in stimulating phosphatidylinositide metabolism. These results indicate that phosphatidylinositide turnover is required for ras-induced meiosis and suggest that phosphatidylinositide-derived second messengers mediate the biological activity of ras in Xenopus oocytes.

Sign in / Sign up

Export Citation Format

Share Document