The Convertible Nucleoside Approach: Structural Engineering of Nucleic Acids by Disulfide Cross-Linking

1994 ◽  
pp. 14-40 ◽  
Author(s):  
A. E. Ferentz ◽  
G. L. Verdine
Keyword(s):  
Nucleic Acids ◽  
Structural Engineering ◽  
Cross Linking ◽  
Convertible Nucleoside

Photochemical Cross-Linking Between Proteins and Nucleic Acids as a Probe of Nucleoprotein Interactions

1980 ◽  
Vol 346 (1 Applications) ◽  
pp. 386-388 ◽  
Author(s):  
Ruth Sperling ◽  
Abraham Havron ◽  
Joseph Sperling
Keyword(s):  
Nucleic Acids ◽  
Cross Linking

CROSS-LINKING BETWEEN NUCLEIC ACIDS AND PROTEINS

1990 ◽  
pp. 297-304
Author(s):  
A. Häckel ◽  
D. Prochnow ◽  
H. Fasold
Keyword(s):  
Nucleic Acids ◽  
Cross Linking

scholarly journals Directing Quinone Methide-Dependent Alkylation and Cross-Linking of Nucleic Acids with Quaternary Amines

2020 ◽  
Vol 31 (5) ◽  
pp. 1486-1496
Author(s):  
Mark A. Hutchinson ◽  
Blessing D. Deeyaa ◽  
Shane R. Byrne ◽  
Sierra J. Williams ◽  
Steven E. Rokita
Keyword(s):  
Nucleic Acids ◽  
Quinone Methide ◽  
Cross Linking

Monospecific Antibody against 5-Methyl-Cytidine for the Structural Analysis of Nucleic Acids

1982 ◽  
Vol 37 (5-6) ◽  
pp. 399-404
Author(s):  
Sofia K. Mastronicolis ◽  
Vassilios M. Kapoulas ◽  
Hans Kröger
Keyword(s):  
Electron Microscopy ◽  
Nucleic Acids ◽  
Density Gradient ◽  
Cross Linking ◽  
Density Gradient Ultracentrifugation ◽  
Disk Electrophoresis ◽  
Detection And Localization ◽  
Hydrolysis Of ◽  
Gel Diffusion ◽  
Drop Technique

Abstract Anti 5-methyl-cytidine antibodies might be useful agents for the detection and localization of 5-methyl-cytidine of nucleic acids, but only if the antibodies recognize this nucleoside with sufficient specificity. A conjugate containing 18 moles of 5-methyl-cytidine per mole of BSA was prepared and antibodies directed against this nucleoside hapten were produced by immunization of rabbits (as determined by gel diffusion in agar containing excessive amounts of the carrier). A slight crossreaction of cytidine-BSA was eliminated by adsorption on the cross-reacting antigen. Further purification of the antibodies was effected by chromatography on DEAE-Sephadex A-50 and a method for the rapid quantitation of the antibodies showed that 12.7% of the IgG protein are monospecific against 5-methyl-cytidine-BSA. Hydrolysis of antibodies with insolubilized pa­pain produced monovalent Fab fragments which were identified by SDS-Disk-electrophoresis. A two stage method for cross linking the immunoproteins to ferritin by glutaraldehyde was used. The isolation of immunoferritin conjugates by Bio-Gel A 1.5 m column chromatography is described. The identification of the effluents was made by glycerin density gradient ultracentrifugation. The results were visualized by electron microscopy after the treatment of immunoferritin conjugates with (methylated and unmethylated) denaturated DNA, fractionation on the glycerine density gradient, and the spreading by a modification of drop technique.

Cross-linking of nucleic acids to proteins. Modified poly(A) as mRNA for Escherichia coli ribosomes

1994 ◽  
Vol 223 (3) ◽  
pp. 799-803 ◽  
Author(s):  
Eva WOJTECH ◽  
Richard BRIMACOMBE ◽  
Andreas HACKEL ◽  
Detlef PROCHNOW ◽  
Hugo FASOLD
Keyword(s):  
Escherichia Coli ◽  
Nucleic Acids ◽  
Cross Linking

scholarly journals Thermodynamic and structural characterization of 2′-nitrogen-modified RNA duplexes

2004 ◽  
Vol 32 (11) ◽  
pp. 3446-3455 ◽  
Author(s):  
John W. Pham ◽  
Ishwar Radhakrishnan ◽  
Erik J. Sontheimer
Keyword(s):  
Molecular Modeling ◽  
Nmr Spectroscopy ◽  
Nucleic Acids ◽  
Chemical Modification ◽  
Structural Characterization ◽  
Cross Linking ◽  
Base Pairing ◽  
Alkyl Groups ◽  
Rna Duplexes

Abstract 2′-aminonucleosides are commonly used as sites of post-synthetic chemical modification within nucleic acids. As part of a larger cross-linking strategy, we appended alkyl groups onto the N2′ position of 2′-amino-modified RNAs via 2′-ureido and 2′-amido linkages. We have characterized the thermodynamics of 2′-amino, 2′-alkylamido and 2′-alkylureido-modified RNA duplexes and show that 2′-ureido-modified RNAs are significantly more stable than analogous 2′-amido-modified RNAs. Using NMR spectroscopy and NMR-based molecular modeling of 2′-modified RNA duplexes, we examined the effects that 2′-nitrogen modifications have on RNA helices. Our data suggest that the 2′-ureido group forms a specific intra-nucleoside interaction that cannot occur within 2′-amido-modified helices. These results indicate that 2′-ureido modifications are superior to analogous 2′-amido ones for applications that require stable base pairing.

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