Extraction and Characterization of Collagen Obtained from Collagen-Containing Wastes of the Leather Industry

The study presents the extraction of collagen, a product of high value, from fleshings form hides. After testing several collagen extraction procedures we have proposed the simple and effective method to extract collagen from collagen-containing wastes of the leather industry. The unified method is based on the extraction of collagen using acetic acid in the presence of EDTA and included two repeated extraction stages. Qualitative analysis of the collagen using the disk-electrophoresis method showed a different ratio of monomers, dimers and other proteins.

Monospecific Antibody against 5-Methyl-Cytidine for the Structural Analysis of Nucleic Acids

Anti 5-methyl-cytidine antibodies might be useful agents for the detection and localization of 5-methyl-cytidine of nucleic acids, but only if the antibodies recognize this nucleoside with sufficient specificity. A conjugate containing 18 moles of 5-methyl-cytidine per mole of BSA was prepared and antibodies directed against this nucleoside hapten were produced by immunization of rabbits (as determined by gel diffusion in agar containing excessive amounts of the carrier). A slight crossreaction of cytidine-BSA was eliminated by adsorption on the cross-reacting antigen. Further purification of the antibodies was effected by chromatography on DEAE-Sephadex A-50 and a method for the rapid quantitation of the antibodies showed that 12.7% of the IgG protein are monospecific against 5-methyl-cytidine-BSA. Hydrolysis of antibodies with insolubilized pa­pain produced monovalent Fab fragments which were identified by SDS-Disk-electrophoresis. A two stage method for cross linking the immunoproteins to ferritin by glutaraldehyde was used. The isolation of immunoferritin conjugates by Bio-Gel A 1.5 m column chromatography is described. The identification of the effluents was made by glycerin density gradient ultracentrifugation. The results were visualized by electron microscopy after the treatment of immunoferritin conjugates with (methylated and unmethylated) denaturated DNA, fractionation on the glycerine density gradient, and the spreading by a modification of drop technique.

Low molecular weight urine proteins in cardiac pathology

To study the nature of proteinuria in the pathology of the cardiovascular system, we used disk electrophoresis in polyacrylamide gel in the presence of sodium dodecyl sulfate. The urine was subjected to electrophoresis without prior preservation. When the protein content in urine is less than 0.3 g / l, it was concentrated 16-30 times with polyethylene glycol. Densitometry of gel pillars was performed on an EPI-65M apparatus (GDR). The glomerular-tubular protein ratio (GTPK) was also calculated.