Metabolic effects of low-flow ischemia on the perfused hypertrophied rat heart: a phosphorus (31P) nuclear magnetic resonance (NMR) study

1983 ◽  
pp. 228-234
Author(s):  
N. Lavanchy ◽  
J. Martin ◽  
J. Aussedat ◽  
A. Ray ◽  
A. Rossi
Keyword(s):  
Nuclear Magnetic Resonance ◽  
Magnetic Resonance ◽  
Rat Heart ◽  
Metabolic Effects ◽  
Low Flow ◽  
31P Nuclear Magnetic Resonance ◽  
Nmr Study ◽  
Nuclear Magnetic

31P-nuclear magnetic resonance analysis of perfused single frog skins

1985 ◽  
Vol 248 (1) ◽  
pp. C177-C180 ◽  
Author(s):  
L. E. Lin ◽  
M. Shporer ◽  
M. M. Civan
Keyword(s):  
Nuclear Magnetic Resonance ◽  
Magnetic Resonance ◽  
Intracellular Ph ◽  
Nmr Spectra ◽  
Nuclear Magnetic Resonance Analysis ◽  
Resonance Analysis ◽  
31P Nuclear Magnetic Resonance ◽  
Nmr Study ◽  
Order Of Magnitude ◽  
Nuclear Magnetic

Perfusion of single frog skins has produced stability of the 31P-nuclear magnetic resonance (NMR) spectra over periods as long as 8 h at room temperature. With this approach, relatively large phosphocreatine (PCr) signals were recorded for each of the 12 frog skins studied. The ratio of the concentration of PCr to ATP was estimated to be 0.76 +/- 0.07, a value an order of magnitude larger than that previously reported. Comparison of the ratio of the intracellular concentrations of inorganic phosphate (Pi) to ATP determined in the present NMR study with that previously estimated by chemical analysis suggests that little intracellular Pi is likely to be immobilized. Inclusion of methylphosphonate in the perfusing solutions permitted simultaneous determination of extracellular and intracellular pH from the NMR spectra alone. The methylphosphonate has been found to be nontoxic. At an extracellular pH of 7.72 +/- 0.04, the intracellular pH was 7.47 +/- 0.06.

In vivo 31P nuclear magnetic resonance (NMR) study of cerebral metabolism during histotoxic hypoxia in mice

1988 ◽  
Vol 3 (1) ◽  
pp. 37-48 ◽  
Author(s):  
Michel Peres ◽  
Philippe Meric ◽  
Bertrand Barrere ◽  
Corinne Pasquier ◽  
Guy Beranger ◽  
...  
Keyword(s):  
Nuclear Magnetic Resonance ◽  
Magnetic Resonance ◽  
Cerebral Metabolism ◽  
31P Nuclear Magnetic Resonance ◽  
Nmr Study ◽  
Histotoxic Hypoxia ◽  
Nuclear Magnetic

31P nuclear magnetic resonance study of the effects of the calcium ion channel antagonist fantofarone on the rat heart

1994 ◽  
Vol 251 (2-3) ◽  
pp. 163-172 ◽  
Author(s):  
Luce Vander Elst ◽  
Pierre Chatelain ◽  
Allan S. Manning ◽  
René Laruel ◽  
Yves Van Haverbeke ◽  
...  
Keyword(s):  
Nuclear Magnetic Resonance ◽  
Magnetic Resonance ◽  
Ion Channel ◽  
Calcium Ion ◽  
Rat Heart ◽  
Nuclear Magnetic Resonance Study ◽  
Magnetic Resonance Study ◽  
31P Nuclear Magnetic Resonance ◽  
Nuclear Magnetic

Metabolic effects of training in humans: a 31P-MRS study

1990 ◽  
Vol 69 (3) ◽  
pp. 1165-1170 ◽  
Author(s):  
J. A. Kent-Braun ◽  
K. K. McCully ◽  
B. Chance
Keyword(s):  
Nuclear Magnetic Resonance ◽  
Magnetic Resonance ◽  
Training Program ◽  
Energy Cost ◽  
Performance Test ◽  
High Energy ◽  
Metabolic Effects ◽  
Ramp Test ◽  
31P Nuclear Magnetic Resonance ◽  
Nuclear Magnetic

The purpose of this study was to determine the feasibility of measuring with 31P nuclear magnetic resonance the effects of an endurance training program on the high-energy phosphate metabolism of exercising human skeletal muscle. The system used included a 1.9-T 30-cm-bore Oxford Systems superconducting magnet, a PhosphoEnergetics spectrometer, and a modified Cybex isokinetic ergometer. Seven healthy human volunteers exercised their wrist flexor muscles 20 min/day 5 days/wk for 8 wk. Testing before and after the training period consisted of a performance test to measure muscle functional capacity and a ramp test to measure the work-energy cost relationship of the exercising muscles. The results indicate that the subjects had a significant increase in their work output on the 10-min performance test after training. They also exhibited an increase in the work-energy cost relationship on the ramp test as indicated by a decrease in peak Pi-to-phosphocreatine ratio and an increase in pH at the same relative power output after training. These results indicate that 1) the training program was sufficient to elicit a training effect and 2) this effect was observed with 31P nuclear magnetic resonance as an increased potential for oxidative metabolism, particularly at the high exercise levels.

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