Covalently Capturing Protein Interactions in Living Cells by Site-Specific Incorporation of Photo-Cross-Linkable Amino Acids

2017 ◽  
pp. 159-181
Author(s):  
Nobumasa Hino ◽  
Kensaku Sakamoto
Keyword(s):  
Amino Acids ◽  
Protein Interactions ◽  
Living Cells ◽  
Site Specific

Engineering an acetyllysine reader with photocrosslinking amino acid for interactome profiling

2021 ◽  
Author(s):  
Babu Sudhamalla ◽  
Anirban Roy ◽  
Soumen Barman ◽  
Jyotirmayee Padhan
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Protein Interactions ◽  
Unnatural Amino Acids ◽  
Protein Protein Interactions ◽  
Site Specific ◽  
Powerful Approach

The site-specific installation of light-activable crosslinker unnatural amino acids offers a powerful approach to trap transient protein-protein interactions both in vitro and in vivo. Herein, we engineer a bromodomain to...

scholarly journals Bifunctional Non-Canonical Amino Acids: Combining Photo-Crosslinking with Click Chemistry

Biomolecules ◽  
2020 ◽  
Vol 10 (4) ◽  
pp. 578 ◽  
Author(s):  
Jan-Erik Hoffmann
Keyword(s):  
Amino Acids ◽  
Genetic Code ◽  
Click Chemistry ◽  
Protein Interactions ◽  
Site Specific ◽  
Genetic Code Expansion

Genetic code expansion is a powerful tool for the study of protein interactions, as it allows for the site-specific incorporation of a photoreactive group via non-canonical amino acids. Recently, several groups have published bifunctional amino acids that carry a handle for click chemistry in addition to the photo-crosslinker. This allows for the specific labeling of crosslinked proteins and therefore the pulldown of peptides for further analysis. This review describes the properties and advantages of different bifunctional amino acids, and gives an overview about current and future applications.

scholarly journals Biosynthetic production of 13C-labeled amino acids with site-specific enrichment.

1982 ◽  
Vol 257 (3) ◽  
pp. 1224-1230
Author(s):  
D.M. LeMaster ◽  
J.E. Cronan
Keyword(s):  
Amino Acids ◽  
Site Specific

scholarly journals Correction to Efficient Site-Specific Prokaryotic and Eukaryotic Incorporation of Halotyrosine Amino Acids into Proteins

2020 ◽  
Author(s):  
Hyo Sang Jang ◽  
Xiaodong Gu ◽  
Richard B. Cooley ◽  
Joseph J. Porter ◽  
Rachel L. Henson ◽  
...  
Keyword(s):  
Amino Acids ◽  
Site Specific

scholarly journals Identification of the target amino acids in the site-specific inactivation of triose phosphate isomerase by ferrate anion.

1983 ◽  
Vol 258 (21) ◽  
pp. 13148-13154 ◽  
Author(s):  
J Steczko ◽  
M Hermodson ◽  
B Axelrod ◽  
E Dziembor-Kentzer
Keyword(s):  
Amino Acids ◽  
Triose Phosphate Isomerase ◽  
Site Specific ◽  
Triose Phosphate
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