Lipase-Catalyzed Reactions for the Fractionation of Fatty Acids

1996 ◽  
pp. 51-64 ◽  
Author(s):  
Kumar D. Mukherjee
Keyword(s):  
Fatty Acids ◽  
Catalyzed Reactions

Synthesis of 2-monoacylglycerols and structured triacylglycerols rich in polyunsaturated fatty acids by enzyme catalyzed reactions

2012 ◽  
Vol 51 (3) ◽  
pp. 148-155 ◽  
Author(s):  
Alicia Rodríguez ◽  
Luis Esteban ◽  
Lorena Martín ◽  
María José Jiménez ◽  
Estrella Hita ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Polyunsaturated Fatty Acids ◽  
Catalyzed Reactions ◽  
Structured Triacylglycerols ◽  
Enzyme Catalyzed Reactions

Rapid, sensitive fluorometric determination of serum triglyceride by measuring lipase-liberated fatty acids

1994 ◽  
Vol 40 (1) ◽  
pp. 14-17 ◽  
Author(s):  
J E Voysey ◽  
D C Wilton
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Serum Triglyceride ◽  
Time Interval ◽  
Serum Samples ◽  
Fluorescence Change ◽  
Displacement Assay ◽  
Catalyzed Reactions ◽  
Hydrolysis Of

Abstract A continuous fluorescence displacement assay was developed for the measurement of long-chain fatty acids and utilized in the study of triglyceride lipase-catalyzed reactions (Wilton, DC. Biochem J 1991;276:129-33). We now describe a method for the rapid measurement of triglyceride in serum with the fluorescence displacement assay. The method involves the hydrolysis of a diluted sample equivalent to 0.1 microL of original serum with excess lipase (EC 3.1.1.3) from Rhizopus arrhizus and measuring the fatty acid released after a set time interval, normally 1 min. Under the conditions of the timed assay, about 2 mol of fatty acid are released per mole of triglyceride. The released fatty acid is monitored by fluorescence change and is proportional to the concentration of triglyceride in the original serum sample. The effective range of serum triglyceride concentration that could be measured was 0.5-10 mmol/L (0.44-8.8 g/L), based on triolein standard. The assay is unique in that it measures lipase-liberated fatty acids rather than liberated glycerol and as such is not subject to many of the criticisms of the glycerol-based methods. Comparison of fasted serum samples established a high correlation between the fatty acid and glycerol methods.

Lipase-catalyzed reactions involving thia fatty acids and ester derivatives

1995 ◽  
Vol 72 (11) ◽  
pp. 1381-1384 ◽  
Author(s):  
Marcel S. F. Lie Ken Jie ◽  
M. S. K. Syed Rahmatullah
Keyword(s):  
Fatty Acids ◽  
Catalyzed Reactions ◽  
Thia Fatty Acids

Lipase-catalyzed reactions of fatty acids with glycerol and acylglycerols

1989 ◽  
Vol 30 (4) ◽  
pp. 332-336 ◽  
Author(s):  
Ricardo Schuch ◽  
Kumar D. Mukherjee
Keyword(s):  
Fatty Acids ◽  
Catalyzed Reactions
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