T-cell Growth Factor-Dependent Leukemic Cell Growth: Therapeutic Implications

Author(s):  
Kendall A. Smith
1993 ◽  
Vol 23 (9) ◽  
pp. 2326-2331 ◽  
Author(s):  
Richard J. Armitage ◽  
Teresa W. Tough ◽  
Brian M. Macduff ◽  
William C. Fanslow ◽  
Melanie K. Spriggs ◽  
...  

Blood ◽  
1984 ◽  
Vol 63 (4) ◽  
pp. 912-916 ◽  
Author(s):  
N Kamatani ◽  
H Yamanaka ◽  
K Nishioka ◽  
T Nakamura ◽  
K Nakano ◽  
...  

Abstract Thioguanine-resistant T lymphoblast populations were selectively amplified using T cell growth factor in the cultures of peripheral blood T cells from four Lesch-Nyhan heterozygotes. Although Lesch-Nyhan T lymphoblasts were all thioguanine-resistant, none of the cultures from 13 control subjects yielded the growth of such defective cell populations. These data provide direct evidence for the existence of a small percentage (5%–40%) of hypoxanthine-guanine phosphoribosyltransferase (HGPRT) deficient T cells in the heterozygotes, but not in normal individuals. Conversely, culture of the T lymphoblasts with azaserine plus hypoxanthine permitted the growth of the other part of the cell population that was enzyme positive. The low percentages of HGPRT-negative cells among T cells in heterozygotes suggest that the presence of this enzyme is beneficial for differentiation of lymphocytes of T cell linkage. Considering the ease and the reliability, culture of the peripheral T cells with thioguanine and T cell growth factor is very likely of practical use for detecting Lesch-Nyhan syndrome carriers among predisposed females.


1980 ◽  
Vol 13 (2) ◽  
pp. 229-241 ◽  
Author(s):  
Francis W. Ruscetti ◽  
James W. Mier ◽  
Robert C. Gallo

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