Determination of Molecular Parameters from Experimental Spectra

2001 ◽  
pp. 107-129
Author(s):  
J. Demaison ◽  
K. Sarka
Keyword(s):  
Molecular Parameters

A determination of the molecular parameters for NiH in its 2Δ ground state by laser magnetic resonance

1991 ◽  
Vol 148 (2) ◽  
pp. 462-478 ◽  
Author(s):  
Thomas Nelis ◽  
Stuart P. Beaton ◽  
Kenneth M. Evenson ◽  
John M. Brown
Keyword(s):  
Magnetic Resonance ◽  
Ground State ◽  
Molecular Parameters ◽  
Laser Magnetic Resonance

Spectroscopie par Source Laser. IV. Application de la Théorie d'Anderson au Calcul des Largeurs des Raies de la Transition 10°0–00°1 de N2O. Influence de l'Octupôle sur la Détermination du Moment Quadrupolaire de N2O

1973 ◽  
Vol 51 (6) ◽  
pp. 605-609 ◽  
Author(s):  
C. Boulet ◽  
N. Lacome ◽  
P. Isnard
Keyword(s):  
Quadrupole Moment ◽  
Experimental Results ◽  
Molecular Parameters ◽  
Line Widths

From the analysis of the available experimental results, a vibrational dependence of N2O line widths is shown, which can be explained, applying the Anderson–Tsao–Curnutte theory. But no dependence on ν of any of the molecular parameters can be deduced. In the last part, we have pointed out the effect on the determination of the quadrupole moment of assuming a nonvanishing octupole moment for N2O.

scholarly journals Experimental Testing of a Size-Exclusion Chromatography Method Used for Evaluation of Molecular Parameters of Equine Anti-Ebola Immunoglobulin

2019 ◽  
Vol 19 (4) ◽  
pp. 261-267
Author(s):  
Е. Yu. Mishalova ◽  
E. V. Gordeev ◽  
V. N. Lebedev ◽  
S. A. Melnikov ◽  
S. A. Nimirskaya ◽  
...  
Keyword(s):  
Specific Activity ◽  
Experimental Testing ◽  
Horse Serum ◽  
Test Procedure ◽  
Size Exclusion ◽  
Array Detector ◽  
Test Procedures ◽  
Molecular Parameters ◽  
Hplc System

Haemorrhagic fever caused by the Ebola virus is a highly hazardous infectious disease with a mortality rate of 50– 90 %. Heterologous immunoglobulins with a high virus-neutralizing titer are an important element of the WHO-endorsed set of measures for emergency prevention and treatment of the disease. Specific activity of these products is largely determined by their fractional composition, and, in particular, by molecular mass distribution (MMD). The size-exclusion-high-performance liquid chromatography (SEC-HPLC) has traditionally been used for determination of the MMD of the target protein in human immunoglobulin-based products. The use of this method for evaluation of molecular parameters of heterologous immunoglobulin requires confirmation of its specificity, accuracy and precision, and establishment of the chromatographic system suitability criteria in the context of a new test object.The aim of the study was to test the applicability of the SEC-HPLC method to the assessment of molecular parameters of anti-Ebola immunoglobulin derived from horse serum.Materials and methods: three batches of purified equine anti-Ebola immunoglobulin were used in the study. Normal equine and human immunoglobulins of the IgG isotype were used as reference standards. The HPLC test procedures described in the European Pharmacopoeia 9.6 and State Pharmacopoeia of the Russian Federation, 14th ed., were used for determination of monomers and other immunoglobulin fractions. An Agilent 1260 Infinity (Agilent, USA) HPLC system with a diode array detector and an Agilent Bio SEC-3 HPLC column were used for quality evaluation of the tested products.Results: the resolution factor between IgG monomer and dimer peaks (1.69 and 2.10), and the chromatographic column efficiency (>2000) make it possible to use the SEC-HPLC system for evaluation of molecular parameters of heterologous immunoglobulin. The study demonstrated reproducibility of the test procedure.Conclusions: the study confirmed the applicability of the SEC-HPLC procedure for evaluation of molecular parameters of anti-Ebola immunoglobulin derived from horse serum. It demonstrated the compliance of the purified immunoglobulin to the national and international quality requirements in terms of «Molecular parameters».

Rapid Determination of Molecular Parameters of Synthetic Polymers Using Precipitation-Redissolution HPLC and a “Molded” Monolithic Column

2003 ◽  
pp. 155-186
Author(s):  
David Sýkora ◽  
Frantisek Svec ◽  
Jean M. J. Fréchet ◽  
Miroslav Petro ◽  
Adam L. Safir
Keyword(s):  
Rapid Determination ◽  
Monolithic Column ◽  
Synthetic Polymers ◽  
Molecular Parameters
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