Structure-Function Relationships in the Membrane Channel Porin as Based on a 1.8 Å Resolution Crystal Structure

Author(s):  
Georg E. Schulz

PLoS ONE ◽  
2014 ◽  
Vol 9 (6) ◽  
pp. e97475 ◽  
Author(s):  
Hsiang-Ting Lei ◽  
Tsung-Han Chou ◽  
Chih-Chia Su ◽  
Jani Reddy Bolla ◽  
Nitin Kumar ◽  
...  


2007 ◽  
Vol 47 (supplement) ◽  
pp. S118
Author(s):  
Hiroshi Kida ◽  
Yuri Sugano ◽  
Ryo Iizuka ◽  
Masahiro Fujihashi ◽  
Masafumi Yohda ◽  
...  


2014 ◽  
Vol 23 (7) ◽  
pp. 954-961 ◽  
Author(s):  
Chih-Chia Su ◽  
Abhijith Radhakrishnan ◽  
Nitin Kumar ◽  
Feng Long ◽  
Jani Reddy Bolla ◽  
...  


eLife ◽  
2018 ◽  
Vol 7 ◽  
Author(s):  
Juan Feng ◽  
Xianchi Dong ◽  
Jennifer Pinello ◽  
Jun Zhang ◽  
Chafen Lu ◽  
...  

HAP2 is a class II gamete fusogen in many eukaryotic kingdoms. A crystal structure of Chlamydomonas HAP2 shows a trimeric fusion state. Domains D1, D2.1 and D2.2 line the 3-fold axis; D3 and a stem pack against the outer surface. Surprisingly, hydrogen-deuterium exchange shows that surfaces of D1, D2.2 and D3 closest to the 3-fold axis are more dynamic than exposed surfaces. Three fusion helices in the fusion loops of each monomer expose hydrophobic residues at the trimer apex that are splayed from the 3-fold axis, leaving a solvent-filled cavity between the fusion loops in each monomer. At the base of the two fusion loops, Arg185 docks in a carbonyl cage. Comparisons to other structures, dynamics, and the greater effect on Chlamydomonas gamete fusion of mutation of axis-proximal than axis-distal fusion helices suggest that the apical portion of each monomer could tilt toward the 3-fold axis with merger of the fusion helices into a common fusion surface.



Biochemistry ◽  
2002 ◽  
Vol 41 (37) ◽  
pp. 11134-11142 ◽  
Author(s):  
G. Parsiegla ◽  
A. Belaïch ◽  
J. P. Belaïch ◽  
R. Haser


2015 ◽  
Vol 5 (1) ◽  
Author(s):  
Bert van den Berg ◽  
Satya Prathyusha Bhamidimarri ◽  
Mathias Winterhalter




1993 ◽  
Vol 49 (s1) ◽  
pp. c109-c110
Author(s):  
M. W. Parker ◽  
J. T. Buckley ◽  
A. D. Tucker ◽  
K. Leonard ◽  
D. Tsernoglou


Sign in / Sign up

Export Citation Format

Share Document