Detection of Avirulent Mutants of Agrobacterium Tumefaciens in Crown-Gall Tumors Produced in Vitro

1993 ◽  
pp. 97-101 ◽  
Author(s):  
C. Bélanger ◽  
M. L. Canfield ◽  
L. W. Moore ◽  
P. Dion
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Crown Gall ◽  
Crown Gall Tumors

Agrobacterium Tumefaciens: What Segment of the Plasmid is Responsible for the Induction of Crown Gall Tumors ?

1977 ◽  
pp. 329-342 ◽  
Author(s):  
J. Schell ◽  
M. Van Montagu ◽  
A. De Picker ◽  
D. De Waele ◽  
G. Engler ◽  
...  
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Crown Gall ◽  
Crown Gall Tumors

A sensitive fluorographic method for the detection of nopaline and octopine synthase activities in Brassica crown-gall tissues

1986 ◽  
Vol 64 (2) ◽  
pp. 126-132 ◽  
Author(s):  
Larry A. Holbrook ◽  
Margaret Haffner ◽  
Brian L. A. Miki
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Crown Gall ◽  
Brassica Species ◽  
Paper Electrophoresis ◽  
In Vitro Assay ◽  
In Vitro Assays ◽  
Tissue Samples ◽  
Vitro Assay ◽  
Ti Plasmids

L-[guanido-14C]Arginine has been used as a precursor for nopaline and octopine syntheses in the in vitro assay for opine synthase activities. Fluorographic detection of [14C]nopaline and [14C]octopine was optimized following their separation from other labelled compounds by paper electrophoresis. Compared with detection by phenanthrenequinone, fluorography was more specific for the guanidine compounds and severalfold more sensitive. Alternate precursors such as α-[14C]ketoglutarate or [14C]pyruvate were poor substitutes for [14C]arginine because they generated compounds that comigrated with opines. Four nonopine radioactive spots recurred in the in vitro assays. These have not been identified but have been distinguished from both octopine and nopaline. This technique has provided clear evidence for transformation of Brassica species by Ti plasmids of Agrobacterium tumefaciens and will be valuable in the analysis of extremely small tissue samples.

scholarly journals Agrobacterium tumefaciens DNA and PS8 Bacteriophage DNA Not Detected in Crown Gall Tumors

1974 ◽  
Vol 71 (9) ◽  
pp. 3672-3676 ◽  
Author(s):  
M.-D. Chilton ◽  
T. C. Currier ◽  
S. K. Farrand ◽  
A. J. Bendich ◽  
M. P. Gordon ◽  
...  
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Crown Gall ◽  
Crown Gall Tumors
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