Laboratory Equipments Used for Host–Plant Resistance Studies

2019 ◽  
pp. 47-52
Author(s):  
K. P. Kumar ◽  
Christine Ilemut
Keyword(s):  
Host Plant ◽  
Plant Resistance ◽  
Host Plant Resistance

scholarly journals Morphological and Chemical Factors Related to Western Flower Thrips Resistance in the Ornamental Gladiolus

Plants ◽  
2021 ◽  
Vol 10 (7) ◽  
pp. 1384
Author(s):  
Dinar S. C. Wahyuni ◽  
Young Hae Choi ◽  
Kirsten A. Leiss ◽  
Peter G. L. Klinkhamer
Keyword(s):  
Host Plant ◽  
Plant Resistance ◽  
Host Plant Resistance ◽  
Frankliniella Occidentalis ◽  
Western Flower Thrips ◽  
Resistance Breeding ◽  
Breeding Programs ◽  
Triterpenoid Saponins ◽  
Flower Thrips ◽  
Highly Correlated

Understanding the mechanisms involved in host plant resistance opens the way for improved resistance breeding programs by using the traits involved as markers. Pest management is a major problem in cultivation of ornamentals. Gladiolus (Gladiolus hybridus L.) is an economically important ornamental in the Netherlands. Gladiolus is especially sensitive to attack by western flower thrips (Frankliniella occidentalis (Pergande) (Thysanoptera:Thripidae)). The objective of this study was, therefore, to investigate morphological and chemical markers for resistance breeding to western flower thrips in Gladiolus varieties. We measured thrips damage of 14 Gladiolus varieties in a whole-plant thrips bioassay and related this to morphological traits with a focus on papillae density. Moreover, we studied chemical host plant resistance to using an eco-metabolomic approach comparing the 1H NMR profiles of thrips resistant and susceptible varieties representing a broad range of papillae densities. Thrips damage varied strongly among varieties: the most susceptible variety showed 130 times more damage than the most resistant one. Varieties with low thrips damage had shorter mesophylls and epidermal cells, as well as a higher density of epicuticular papillae. All three traits related to thrips damage were highly correlated with each other. We observed a number of metabolites related to resistance against thrips: two unidentified triterpenoid saponins and the amino acids alanine and threonine. All these compounds were highly correlated amongst each other as well as to the density of papillae. These correlations suggest that papillae are involved in resistance to thrips by producing and/or storing compounds causing thrips resistance. Although it is not possible to distinguish the individual effects of morphological and chemical traits statistically, our results show that papillae density is an easy marker in Gladiolus-breeding programs targeted at increased resistance to thrips.

scholarly journals Comparative TMT Proteomic Analysis Unveils Unique Insights into Helicoverpa armigera (Hübner) Resistance in Cajanus scarabaeoides (L.) Thouars

2021 ◽  
Vol 22 (11) ◽  
pp. 5941
Author(s):  
Abigail Ngugi-Dawit ◽  
Isaac Njaci ◽  
Thomas J.V. Higgins ◽  
Brett Williams ◽  
Sita R. Ghimire ◽  
...  
Keyword(s):  
Host Plant ◽  
Helicoverpa Armigera ◽  
Plant Resistance ◽  
Host Plant Resistance ◽  
Proteomic Analysis ◽  
Artificial Diet ◽  
Growth Stages ◽  
Genetic Base ◽  
Helicoverpa Armígera ◽  
Helicoverpa Armigera Hubner

Pigeonpea [Cajanus cajan (L.) Millspaugh] is an economically important legume playing a crucial role in the semi-arid tropics. Pigeonpea is susceptible to Helicoverpa armigera (Hübner), which causes devastating yield losses. This pest is developing resistance to many commercially available insecticides. Therefore, crop wild relatives of pigeonpea, are being considered as potential sources of genes to expand the genetic base of cultivated pigeonpea to improve traits such as host plant resistance to pests and pathogens. Quantitative proteomic analysis was conducted using the tandem mass tag platform to identify differentially abundant proteins between IBS 3471 and ICPL 87 tolerant accession and susceptible variety to H. armigera, respectively. Leaf proteome were analysed at the vegetative and flowering/podding growth stages. H. armigera tolerance in IBS 3471 appeared to be related to enhanced defence responses, such as changes in secondary metabolite precursors, antioxidants, and the phenylpropanoid pathway. The development of larvae fed on an artificial diet with IBS 3471 lyophilised leaves showed similar inhibition with those fed on an artificial diet with quercetin concentrations with 32 mg/25 g of artificial diet. DAB staining (3,3’-diaminobenzidine) revealed a rapid accumulation of reactive oxygen species in IBS 3471. We conclude that IBS 3471 is an ideal candidate for improving the genetic base of cultivated pigeonpea, including traits for host plant resistance.

scholarly journals Deciphering the antagonistic effect of Streptomyces spp. and host-plant resistance induction against charcoal rot of sorghum

Planta ◽  
2021 ◽  
Vol 253 (2) ◽  
Author(s):  
Subramaniam Gopalakrishnan ◽  
Vadlamudi Srinivas ◽  
Nimmala Naresh ◽  
Sambangi Pratyusha ◽  
Sravani Ankati ◽  
...  
Keyword(s):  
Host Plant ◽  
Plant Resistance ◽  
Host Plant Resistance ◽  
Antagonistic Effect ◽  
Charcoal Rot ◽  
Resistance Induction ◽  
Streptomyces Spp

A Review of Host Plant Resistance of Pecan, Carya illinoensis, to Insecta and Acarina

1979 ◽  
Vol 25 (4) ◽  
pp. 251-257 ◽  
Author(s):  
T. L. Carpenter ◽  
W. W. Neel ◽  
P. A. Hedin
Keyword(s):  
Host Plant ◽  
Plant Resistance ◽  
Host Plant Resistance ◽  
Carya Illinoensis

Electrical penetration graphs combined with stylet cutting in the study of host plant resistance to aphids

1984 ◽  
Vol 35 (2) ◽  
pp. 210-213 ◽  
Author(s):  
P. J. M. Mentink ◽  
F. M. Kimmins ◽  
P. Harrewijn ◽  
F. L. Dieleman ◽  
W. F. Tjallingii ◽  
...  
Keyword(s):  
Host Plant ◽  
Plant Resistance ◽  
Host Plant Resistance ◽  
Electrical Penetration Graphs
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