Application of Single-Cell RNA Sequencing in Pancreatic Cancer and the Endocrine Pancreas

2020 ◽  
pp. 143-152
Author(s):  
Qiankun Luo ◽  
Qiang Fu ◽  
Xu Zhang ◽  
Hongwei Zhang ◽  
Tao Qin
Keyword(s):  
Pancreatic Cancer ◽  
Single Cell ◽  
Rna Sequencing ◽  
Endocrine Pancreas ◽  
Single Cell Rna Sequencing

scholarly journals Critical changes in hypothalamic gene networks in response to pancreatic cancer as found by single-cell RNA sequencing

2022 ◽  
pp. 101441
Author(s):  
Christian Huisman ◽  
Mason A. Norgard ◽  
Peter R. Levasseur ◽  
Stephanie M. Krasnow ◽  
Monique G.P. van der Wijst ◽  
...  
Keyword(s):  
Pancreatic Cancer ◽  
Single Cell ◽  
Rna Sequencing ◽  
Gene Networks ◽  
Single Cell Rna Sequencing

Abstract NG04: Diversity of circulating tumor cells in a mouse pancreatic cancer model identified by single cell RNA sequencing

2014 ◽  
Author(s):  
David T. Ting ◽  
Ben S. Wittner ◽  
Ajay M. Shah ◽  
David T. Miyamoto ◽  
Brian W. Brannigan ◽  
...  
Keyword(s):  
Pancreatic Cancer ◽  
Single Cell ◽  
Tumor Cells ◽  
Rna Sequencing ◽  
Circulating Tumor Cells ◽  
Cancer Model ◽  
Single Cell Rna Sequencing

scholarly journals Single-cell RNA sequencing to characterize the response of pancreatic cancer to anti-PD-1 immunotherapy

2022 ◽  
Vol 15 (1) ◽  
pp. 101262
Author(s):  
Jing Zhou ◽  
Yuexu Jiang ◽  
Yue Huang ◽  
Qiongling Wang ◽  
Jussuf T. Kaifi ◽  
...  
Keyword(s):  
Pancreatic Cancer ◽  
Single Cell ◽  
Rna Sequencing ◽  
Single Cell Rna Sequencing

72 - Single Cell RNA Sequencing Points to Altered IL-10 Signalling and Metabolism in Hyperinsulinemia-Driven Pancreatic Cancer Initiation

2020 ◽  
Vol 44 (7) ◽  
pp. S30
Author(s):  
Anni Zhang ◽  
Twan De Winter ◽  
Xioake Hu ◽  
Hong Li ◽  
Janel Kopp ◽  
...  
Keyword(s):  
Pancreatic Cancer ◽  
Single Cell ◽  
Rna Sequencing ◽  
Cancer Initiation ◽  
Single Cell Rna Sequencing

scholarly journals Endocrine Pancreas Development and Dysfunction Through the Lens of Single-Cell RNA-Sequencing

2021 ◽  
Vol 9 ◽  
Author(s):  
Wojciech J. Szlachcic ◽  
Natalia Ziojla ◽  
Dorota K. Kizewska ◽  
Marcelina Kempa ◽  
Malgorzata Borowiak
Keyword(s):  
Blood Glucose ◽  
Single Cell ◽  
Rna Sequencing ◽  
Endocrine Pancreas ◽  
Pancreas Development ◽  
Β Cells ◽  
Β Cell ◽  
Regulatory Pathways ◽  
Endocrine Differentiation ◽  
Single Cell Rna Sequencing

A chronic inability to maintain blood glucose homeostasis leads to diabetes, which can damage multiple organs. The pancreatic islets regulate blood glucose levels through the coordinated action of islet cell-secreted hormones, with the insulin released by β-cells playing a crucial role in this process. Diabetes is caused by insufficient insulin secretion due to β-cell loss, or a pancreatic dysfunction. The restoration of a functional β-cell mass might, therefore, offer a cure. To this end, major efforts are underway to generate human β-cells de novo, in vitro, or in vivo. The efficient generation of functional β-cells requires a comprehensive knowledge of pancreas development, including the mechanisms driving cell fate decisions or endocrine cell maturation. Rapid progress in single-cell RNA sequencing (scRNA-Seq) technologies has brought a new dimension to pancreas development research. These methods can capture the transcriptomes of thousands of individual cells, including rare cell types, subtypes, and transient states. With such massive datasets, it is possible to infer the developmental trajectories of cell transitions and gene regulatory pathways. Here, we summarize recent advances in our understanding of endocrine pancreas development and function from scRNA-Seq studies on developing and adult pancreas and human endocrine differentiation models. We also discuss recent scRNA-Seq findings for the pathological pancreas in diabetes, and their implications for better treatment.

scholarly journals Elucidation of tumor-stromal heterogeneity and the ligand-receptor interactome by single cell transcriptomics in real-world pancreatic cancer biopsies

2020 ◽  
Author(s):  
Jaewon J. Lee ◽  
Vincent Bernard ◽  
Alexander Semaan ◽  
Maria E. Monberg ◽  
Jonathan Huang ◽  
...  
Keyword(s):  
Pancreatic Cancer ◽  
Single Cell ◽  
Rna Sequencing ◽  
Clinical Decision Making ◽  
De Novo ◽  
Ex Vivo ◽  
Ductal Adenocarcinoma ◽  
Precision Oncology ◽  
Single Cell Rna Sequencing

AbstractPrecision medicine approaches in pancreatic ductal adenocarcinoma (PDAC) are imperative for improving disease outcomes. However, the long-term fidelity of recently deployed ex vivo preclinical platforms, such as patient-derived organoids (PDOs) remains unknown. Through single-cell RNA sequencing (scRNA-seq), we identify substantial transcriptomic evolution of PDOs propagated from the parental tumor, which may alter predicted drug sensitivity. In contrast, scRNA-seq is readily applicable to limited biopsies from human primary and metastatic PDAC and identifies most cancers as being an admixture of previously described epithelial transcriptomic subtypes. Integrative analyses of our data provide an in-depth characterization of the heterogeneity within the tumor microenvironment, including cancer-associated fibroblast (CAF) subclasses, and predicts for a multitude of ligand-receptor interactions, revealing potential targets for immunotherapy approaches. While PDOs continue to enable prospective therapeutic prediction, our analysis also demonstrates the complementarity of using orthogonal de novo biopsies from PDAC patients paired with scRNA-seq to inform clinical decision-making.Statement of SignificanceThe application of single-cell RNA sequencing to diagnostic pancreatic cancer biopsies provides in-depth transcriptomic characterization of the tumor epithelium and microenvironment, while minimizing potential artifacts introduced by an intervening ex vivo passaging step. Thus, this approach can complement the use of patient-derived organoids in implementing precision oncology.

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