Double Haploid Production and Its Applications in Crop Improvement

Author(s):  
Awadhesh Kumar Mishra ◽  
Rajesh Saini ◽  
Kavindra Nath Tiwari
2017 ◽  
Vol 77 (3) ◽  
pp. 428 ◽  
Author(s):  
Hanif Khan ◽  
S. C. Bhardwaj ◽  
O. P. Gangwar ◽  
Pramod Prasad ◽  
Ruchi Rathore

Crop Science ◽  
1991 ◽  
Vol 31 (3) ◽  
pp. 723-726 ◽  
Author(s):  
Dhia S. Hassawi ◽  
George H. Liang

Author(s):  
Rashmi Rekha Hazarika ◽  
Vijay Kumar Mishra ◽  
Rakhi Chaturvedi

2020 ◽  
Vol 06 (01) ◽  
pp. 21-27
Author(s):  
Swapan K. Tripathy ◽  
Sambit Patra ◽  
Jayashree Kar

2017 ◽  
Vol 44 (3) ◽  
pp. 221
Author(s):  
Heni Safitri ◽  
Bambang Sapta Purwoko ◽  
Iswari Saraswati Dewi ◽  
Dan Sintho Wahyuning Ardie

<p>ABSTRACT<br /><br />Haploid breeding through anther culture allows shortening of the breeding cycle and production of homozygous lines from a segregating population in the immediate generation. This technique has been used for crop improvement especially in rice. The objective of this research was to determine regeneration ability of twelve F1s, derived from reciprocal crossing between high yielding rice variety and rice tolerance to salinity, through anther culture. Completely randomized design with 20 replications was used in this research. Medium for callus induction was based on N6 medium + 2.0 mg NAA L-1 + 0.5 mg kinetin L-1 + 1 mM putrescine, while regeneration medium was based on MS + 0.5 mg NAA L-1 + 2.0 mg kinetin L-1 + 1 mM putrescine. Rooting were done in MS medium + 0.5 mg IBA L-1 + 1 mM putrescine. The result indicated that F1 derived from IR77674/Inpari 29 (3.1% green plants/total anther) was the most responsive genotypes in rice anther culture (high anther culture ability). After greenhouse grow out 125 putative double haploid plants were obtained (41.5% from total acclimated green plantlets).  <br /><br />Keywords: double haploid, green planlets, indica rice, salt tolerance <br /><br /></p>


Author(s):  
R. B. Austin ◽  
R. B. Flavell ◽  
I. E. Henson ◽  
H. J. B. Lowe

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