Genetic Fidelity Studies for Testing True-to-Type Plants in Some Horticultural and Medicinal Crops Using Molecular Markers

2021 ◽  
pp. 147-170
Author(s):  
Sapna Tyagi ◽  
Deepak Rajpurohit ◽  
Amit Sharma
Keyword(s):  
Molecular Markers ◽  
Genetic Fidelity ◽  
Medicinal Crops

Molecular markers based genetic fidelity assessment of micropropagated Dendrobium chrysotoxum Lindl

Meta Gene ◽  
2019 ◽  
Vol 20 ◽  
pp. 100562 ◽  
Author(s):  
Leimapokpam Tikendra ◽  
Arunkumar Singh Koijam ◽  
Potshangbam Nongdam
Keyword(s):  
Molecular Markers ◽  
Genetic Fidelity ◽  
Fidelity Assessment

Genetic fidelity of long-term micropropagated shoot cultures of vanilla (Vanilla planifolia Andrews) as assessed by molecular markers

2007 ◽  
Vol 2 (8) ◽  
pp. 1007-1013 ◽  
Author(s):  
Reddampalli V. Sreedhar ◽  
Lakshmanan Venkatachalam ◽  
Neelwarne Bhagyalakshmi
Keyword(s):  
Molecular Markers ◽  
Genetic Fidelity ◽  
Shoot Cultures ◽  
Vanilla Planifolia

Morphological and molecular analyses in micropropagated berry plants acclimatized under ex vitro condition

2012 ◽  
Vol 92 (6) ◽  
pp. 1065-1073 ◽  
Author(s):  
S. C. Debnath ◽  
P. Vyas ◽  
J. C. Goyali ◽  
A. U. Igamberdiev
Keyword(s):  
Tissue Culture ◽  
Molecular Markers ◽  
Genetic Fidelity ◽  
Morphological Characters ◽  
Plant Production ◽  
Ex Vitro ◽  
In Vitro Morphogenesis ◽  
Molecular Analyses ◽  
Berry Crops

Debnath, S. C., Vyas, P., Goyali, J. C. and Igamberdiev, A. U. 2012. Morphological and molecular analyses in micropropagated berry plants acclimatized under ex vitro condition. Can. J. Plant Sci. 92: 1065–1073. Berry crops include, but are not limited to, the members of the genera Fragaria (strawberry; Rosaceae), Rubus (brambles: raspberry and blackberry; Rosaceae), Vaccinium (blueberry, cranberry and lingonberry; Ericaceae) and Ribes (currant and gooseberry; Grossulariaceae). While berry fruits have long enjoyed huge popularity among consumers, tremendous progress in plant tissue culture, resulting in great advances in micropropagation, has occurred. The in vitro morphogenesis seems to be highly dependent on plant growth regulators and media used for culture, which is again genotype specific. Although automation of micropropagation in bioreactors has been advanced as a possible way of reducing the cost of propagation, optimal plant production depends on better understanding of physiological and biochemical responses of plants to the signals of the culture microenvironment and an optimization of specific physical and chemical culture conditions to control the morphogenesis of berry plants in liquid culture systems. Increased branching, vigorous vegetative growth and change in biochemical components are often noted in micropropagated plants acclimatized under ex vitro condition. Clonal fidelity can be a serious problem and strategies have been developed to reduce the variation to manageable levels. Molecular markers have been introduced in tissue culture research and can potentially be used in various facets of pertinent studies with berry crops. This paper describes in depth the progress of various aspects of berry propagation in vitro, the characterization of micropropagated berry plants for morphological characters, and the employment of molecular markers in these plants for the assessment of genetic fidelity, uniformity, stability and trueness-to-type among donor plants and tissue culture regenerants.

Bioreactors and molecular analysis in berry crop micropropagation – A review

2011 ◽  
Vol 91 (1) ◽  
pp. 147-157 ◽  
Author(s):  
Samir Debnath
Keyword(s):  
Tissue Culture ◽  
Molecular Markers ◽  
Molecular Analysis ◽  
Genetic Fidelity ◽  
Culture Conditions ◽  
Plant Production ◽  
Solid Media ◽  
Berry Crops ◽  
Berry Fruits

Debnath, S. C. 2011. Bioreactors and molecular analysis in berry crop micropropagation – A review. Can. J. Plant Sci. 91: 147–157. While berry fruits have long enjoyed huge popularity among consumers, tremendous progress in plant tissue culture, resulting in great advances in micropropagation, has occurred. Of particular significance has been the evolution of the technology permitting multiplication of berry plants in bioreactors containing liquid media. Although automation of micropropagation in bioreactors has been advanced as a possible way of reducing propagation cost, optimal plant production depends upon better understanding of physiological and biochemical responses of plant to the signals of culture microenvironment and an optimization of specific physical and chemical culture conditions to control the morphogenesis of berry plants in liquid culture systems. Clonal fidelity can be a serious problem, and molecular strategies have been developed in order to reduce the variation to manageable levels. Molecular markers have been introduced to tissue culture research and can potentially be used in various facets of pertinent studies with berry crops. The paper focuses on bioreactor systems combined with semi-solid media used for in vitro culture of berry crops, cultivation of micropropagules and employment of molecular markers in micropropagated plants for the assessment of genetic fidelity, uniformity, stability and trueness-to-type among donor plants and tissue culture regenerants. The pertinent literature is reviewed and the relative merits and shortcomings of the various molecular markers applied are presented with an emphasis on the nature of tissue culture-induced variation.

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