Bioreactors and molecular analysis in berry crop micropropagation – A review

2011 ◽  
Vol 91 (1) ◽  
pp. 147-157 ◽  
Author(s):  
Samir Debnath
Keyword(s):  
Tissue Culture ◽  
Molecular Markers ◽  
Molecular Analysis ◽  
Genetic Fidelity ◽  
Culture Conditions ◽  
Plant Production ◽  
Solid Media ◽  
Berry Crops ◽  
Berry Fruits

Debnath, S. C. 2011. Bioreactors and molecular analysis in berry crop micropropagation – A review. Can. J. Plant Sci. 91: 147–157. While berry fruits have long enjoyed huge popularity among consumers, tremendous progress in plant tissue culture, resulting in great advances in micropropagation, has occurred. Of particular significance has been the evolution of the technology permitting multiplication of berry plants in bioreactors containing liquid media. Although automation of micropropagation in bioreactors has been advanced as a possible way of reducing propagation cost, optimal plant production depends upon better understanding of physiological and biochemical responses of plant to the signals of culture microenvironment and an optimization of specific physical and chemical culture conditions to control the morphogenesis of berry plants in liquid culture systems. Clonal fidelity can be a serious problem, and molecular strategies have been developed in order to reduce the variation to manageable levels. Molecular markers have been introduced to tissue culture research and can potentially be used in various facets of pertinent studies with berry crops. The paper focuses on bioreactor systems combined with semi-solid media used for in vitro culture of berry crops, cultivation of micropropagules and employment of molecular markers in micropropagated plants for the assessment of genetic fidelity, uniformity, stability and trueness-to-type among donor plants and tissue culture regenerants. The pertinent literature is reviewed and the relative merits and shortcomings of the various molecular markers applied are presented with an emphasis on the nature of tissue culture-induced variation.

Morphological and molecular analyses in micropropagated berry plants acclimatized under ex vitro condition

2012 ◽  
Vol 92 (6) ◽  
pp. 1065-1073 ◽  
Author(s):  
S. C. Debnath ◽  
P. Vyas ◽  
J. C. Goyali ◽  
A. U. Igamberdiev
Keyword(s):  
Tissue Culture ◽  
Molecular Markers ◽  
Genetic Fidelity ◽  
Morphological Characters ◽  
Plant Production ◽  
Ex Vitro ◽  
In Vitro Morphogenesis ◽  
Molecular Analyses ◽  
Berry Crops

Debnath, S. C., Vyas, P., Goyali, J. C. and Igamberdiev, A. U. 2012. Morphological and molecular analyses in micropropagated berry plants acclimatized under ex vitro condition. Can. J. Plant Sci. 92: 1065–1073. Berry crops include, but are not limited to, the members of the genera Fragaria (strawberry; Rosaceae), Rubus (brambles: raspberry and blackberry; Rosaceae), Vaccinium (blueberry, cranberry and lingonberry; Ericaceae) and Ribes (currant and gooseberry; Grossulariaceae). While berry fruits have long enjoyed huge popularity among consumers, tremendous progress in plant tissue culture, resulting in great advances in micropropagation, has occurred. The in vitro morphogenesis seems to be highly dependent on plant growth regulators and media used for culture, which is again genotype specific. Although automation of micropropagation in bioreactors has been advanced as a possible way of reducing the cost of propagation, optimal plant production depends on better understanding of physiological and biochemical responses of plants to the signals of the culture microenvironment and an optimization of specific physical and chemical culture conditions to control the morphogenesis of berry plants in liquid culture systems. Increased branching, vigorous vegetative growth and change in biochemical components are often noted in micropropagated plants acclimatized under ex vitro condition. Clonal fidelity can be a serious problem and strategies have been developed to reduce the variation to manageable levels. Molecular markers have been introduced in tissue culture research and can potentially be used in various facets of pertinent studies with berry crops. This paper describes in depth the progress of various aspects of berry propagation in vitro, the characterization of micropropagated berry plants for morphological characters, and the employment of molecular markers in these plants for the assessment of genetic fidelity, uniformity, stability and trueness-to-type among donor plants and tissue culture regenerants.

scholarly journals Barley somatic embryogenesis-an attempt to modify variation induced in tissue culture

2021 ◽  
Vol 28 (1) ◽  
Author(s):  
Renata Orłowska
Keyword(s):  
Tissue Culture ◽  
Somatic Embryogenesis ◽  
Taguchi Method ◽  
Culture Conditions ◽  
Dna Demethylation ◽  
Silver Ion ◽  
Donor Plant ◽  
In Vitro Tissue Culture ◽  
Induced Variation

Abstract Background Somatic embryogenesis is a phenomenon carried out in an environment that generates abiotic stress. Thus, regenerants may differ from the source of explants at the morphological, genetic, and epigenetic levels. The DNA changes may be the outcome of induction media ingredients (i.e., copper and silver ions) and their concentrations and time of in vitro cultures. Results This study optimised the level of copper and silver ion concentration in culture media parallel with the induction medium longevity step towards obtaining barley regenerants via somatic embryogenesis with a minimum or maximum level of tissue culture-induced differences between the donor plant and its regenerants. The optimisation process is based on tissue culture-induced variation evaluated via the metAFLP approach for regenerants derived under varying in vitro tissue culture conditions and exploited by the Taguchi method. In the optimisation and verification experiments, various copper and silver ion concentrations and the different number of days differentiated the tested trials concerning the tissue culture-induced variation level, DNA demethylation, and de novo methylation, including symmetric (CG, CHG) and asymmetric (CHH) DNA sequence contexts. Verification of optimised conditions towards obtaining regenerants with minimum and maximum variability compared to donor plants proved useful. The main changes that discriminate optimised conditions belonged to DNA demethylation events with particular stress on CHG context. Conclusions The combination of tissue culture-induced variation evaluated for eight experimental trials and implementation of the Taguchi method allowed the optimisation of the in vitro tissue culture conditions towards the minimum and maximum differences between a source of tissue explants (donor plant) and its regenerants from somatic embryos. The tissue culture-induced variation characteristic is mostly affected by demethylation with preferences towards CHG sequence context.

scholarly journals Ex Vitro Rooting of American Chestnut Improves Acclimatization Survival and Plantlet Quality

2016 ◽  
Vol 34 (3) ◽  
pp. 75-79 ◽  
Author(s):  
Allison D Oakes ◽  
Tyler R. Desmarais ◽  
William A. Powell ◽  
Charles A. Maynard
Keyword(s):  
Tissue Culture ◽  
Keystone Species ◽  
Ex Vitro Rooting ◽  
Plant Production ◽  
Shoot Cultures ◽  
Ex Vitro ◽  
Regulatory Review ◽  
Survival Percentage ◽  
Virus Indexing

Tissue culture of plants has many applications, from producing genetically identical horticultural varieties, to production of secondary metabolites, to virus indexing, and most relevantly, developing novel traits by genetic transformation. Using Agrobacterium-mediated transformation on somatic embryos, blight-resistant American chestnuts [Castanea dentata (Marsh.) Borkh.] have been developed as shoot cultures in plant tissue culture. Rooting tissue-cultured shoots and acclimatizing the rooted plantlets are key steps in tree production. In this study, in vitro and ex vitro rooting methods were compared. The ex vitro method resulted in a lower initial rooting percentage but an overall higher survival percentage, resulting in higher potted plant production. The higher survival was likely due to partial acclimatization taking place before the plantlets were transplanted into potting mix. After 8 weeks, plantlets rooted via the ex vitro method were taller, and had more, and larger, leaves than the in vitro-rooted plantlets. These trees are currently in high demand for inoculation studies for federal regulatory review and eventually for restoration of this keystone species to its native habitat.

scholarly journals Micropropagation and callogenesis of Curcuma zedoaria Roscoe

2004 ◽  
Vol 61 (4) ◽  
pp. 427-432 ◽  
Author(s):  
Jeanette Inamine Miachir ◽  
Vera Lúcia Moretti Romani ◽  
Antônio Francisco de Campos Amaral ◽  
Marcia Ometto Mello ◽  
Otto Jesu Crocomo ◽  
...  
Keyword(s):  
Growth And Development ◽  
Cell Suspension Cultures ◽  
Culture Conditions ◽  
Plant Production ◽  
Curcuma Zedoaria ◽  
Plant Growth And Development ◽  
Skoog Medium ◽  
Medicinal Properties ◽  
Benzyl Amino Purine

Curcuma zedoaria Roscoe (zedoary) is a medicinal properties-bearing Zingiberaceae from which rhizomes are commercially exploited. The objective of this work was to establish an in vitro protocol for micropropagation and callogenesis of Curcuma zedoaria Roscoe as alternative to improve plant production, turning economically feasible the exploitation of its secondary metabolites which present medicinal properties. Micropropagation by using shoot apexes produced by rhizome and from in vitro plants were carried out on Murashige & Skoog medium supplemented with 2.0 mg L-1 benzyl amino purine and 30 g L-1 sucrose. Plantlets were satisfactorily acclimated to greenhouse conditions by using plastic cover for at least 10 days. Treatment with endomycorrhiza at the ex vitro transferring time was beneficial to acclimatization, improving plant growth and development. Callus induction and growth were obtained by inoculating root segments on Murashige & Skoog medium supplemented with 1.0 mg L-1 naphtalene acetic acid and incubation in the dark at 25 ± 2ºC. Cell suspension cultures were established on liquid medium of same chemical composition and same culture conditions and a growth curve was obtained.

Isozyme assessment of the genetic stability of micropropagated hybrid larch (Larix × eurolepis Henry)

1995 ◽  
Vol 25 (7) ◽  
pp. 1103-1112 ◽  
Author(s):  
Sylvie Richard ◽  
Sylvie Gauthier ◽  
Sylvie Laliberté
Keyword(s):  
Tissue Culture ◽  
Growth Regulators ◽  
Genetic Stability ◽  
Physiological Response ◽  
Culture Conditions ◽  
Hybrid Larch ◽  
Short Shoot ◽  
In Vitro Shoots ◽  
The Media

The search for the occurrence of somaclonal variation of in vitro shoots and acclimatized plants of a hybrid larch (Larix × urolepis Henry) clone was performed by the analysis of eight isozyme systems. Cultures were established from short shoot buds of mature material. The effects of growth regulators in the media, subculture intervals, and periods in culture were analyzed for in vitro shoots. Variability was found in in vitro shoots but appeared to be related to a physiological response to culture conditions. Once acclimatized, most tissuecultured plants expressed the same enzymatic patterns as those of control plants (stecklings and the ortet). The variations observed for some acclimatized plants were also observed in control plants and were not related to ontogenic stage. Results from the isoenzymatic systems studied showed that hybrid larch plants regenerated from tissue culture were not significantly different from stecklings and the ortet.

scholarly journals The effects of linght emitting diode lighting on growth and development of A. annanesis and A. roxburghii in vitro cultured shoots

2017 ◽  
Vol 40 (1) ◽  
pp. 32-38
Author(s):  
Phan Xuan Binh Minh ◽  
Bui Thi Thanh Phuong ◽  
Pham Huong Son ◽  
Tran Minh Hoi ◽  
Nguyen Thi Phuong Lan ◽  
...  
Keyword(s):  
Growth And Development ◽  
Photosynthetic Photon Flux Density ◽  
Culture Conditions ◽  
Plant Production ◽  
Photon Flux ◽  
Photon Flux Density ◽  
Endangered Plants ◽  
Operating Life ◽  
Suitable Habitats

A. annamensis and A. roxburghii belong to Orchidaceae family that has medicinal and ornamental plant value. They are in extinct endangered plants in wild due to the over- collected and loss of the suitable habitats. Using the LED lighting source for culture these species in in vitro condition to optimize the culture conditions, reduction of the production cost, especially electric bill for air-corditionning, lighting. In recent years, the trial applied LED which has the feature of energy saving, small size and a longer operating life, for plant production has started. In this study, LED illumination sources are in four different wavelengths of λ= 430- 470 nm; λ= 470-510 nm; λ= 510-560 nm; λ= 560-600 nm and white fluorescent lamp as control with light intensity photosynthetic photon flux density (PPFD) of 40 µmol/m2/s photon used to study their effects on the growth and development of A. annamensis and A. roxburghii species. After 8 weeks of implementing, the results showed that the LEDs of λ= 470-510 nm were suitable for the growth and development for A. roxburghii shoots while for A. annamensis, λ = 430- 470 nm were most suitable for budding and λ= 470-510 nm for shoot growth. Citation: Phan Xuan Binh Minh, Bui Thi Thanh Phuong, Pham Huong Son, Tran Minh Hoi, Nguyen Thi Phuong Lan, Vu Thi Thao, 2018. The effects of linght emitting diode lighting on growth and development of A. annanesis and A. roxburghii in vitro cultured shoots. Tap chi Sinh hoc, 40(1): x-xx. DOI: 10.15625/0866-7160/v40n1.10636. *Corresponding author: [email protected] Received 23 August 2017, accepted 2 December 2017

scholarly journals Effects of Polyamines And Silver Thiosulphate On Phoenix Dactylifera L. Cv. Quntar Micropropagation And Molecular Analysis.

2021 ◽  
Author(s):  
Ahmed Almayahi
Keyword(s):  
Tissue Culture ◽  
Date Palm ◽  
Genetic Fidelity ◽  
Phoenix Dactylifera ◽  
Silver Thiosulfate ◽  
Practical Applications ◽  
Root Regeneration ◽  
Phoenix Dactylifera L ◽  
Fidelity Assessment

Abstract There are some limitations in the practical applications of in vitro date palm tissue culture, such as low multiplication efficiency, low rooting rate, and high mortality experienced by in vitro raised plantlets during laboratory to soil transfer. The objective of the present study is to determine the effect of polyamines (putrescine "PUT" and spermidine" SPD") and silver thiosulfate (STS) on enhancing propagation of date palm cv Quntar in vitro. Media supplemented with 75 mg L-1 SPD in combination with 10 mgL-1 STS gave the highest percentage of callus producing buds (83.34%) and average bud formation (16.3) per jar. The addition of PUT and STS to the medium was most effective in root regeneration and the number of roots per shoot, where the best result 91.67% and 6.37 roots per shoot, respectively, were obtained using 75 mgL-1 PUT and 10 mgL-1 STS, resulting in fast-growing plantlets during acclimatization phase, reaching 90% of plant survival. The genetic fidelity assessment of plants derived from micropropagation was confirmed by RAPD analysis. Four operon primers were used, and all of them showed amplified unambiguous (OPA02, OPC-04, OPD-07, and OPE-15). All generated bands were monomorphic and had no variation among the tissue culture-derived plants tested. Accordingly, these results indicate that adding polyamines and silver thiosulfate to the nutrient medium of date palm cv. Quntar is beneficial in improving shoot organogenesis, rooting, and production of genetically stable date palm plants.

Sign in / Sign up

Export Citation Format

Share Document