scholarly journals Molecular markers : An important tool to assess genetic fidelity in tissue culture grown long-term cultures of economically important fruit plants

2015 ◽  
Vol 10 (1) ◽  
pp. 101-105 ◽  
Author(s):  
MANISH CHITTORA ◽  
DHAWNI SHARMA ◽  
CHANDRA VEER ◽  
GARIMA VERMA
Keyword(s):  
Tissue Culture ◽  
Molecular Markers ◽  
Genetic Fidelity ◽  
Fruit Plants

Genetic fidelity of long-term micropropagated shoot cultures of vanilla (Vanilla planifolia Andrews) as assessed by molecular markers

2007 ◽  
Vol 2 (8) ◽  
pp. 1007-1013 ◽  
Author(s):  
Reddampalli V. Sreedhar ◽  
Lakshmanan Venkatachalam ◽  
Neelwarne Bhagyalakshmi
Keyword(s):  
Molecular Markers ◽  
Genetic Fidelity ◽  
Shoot Cultures ◽  
Vanilla Planifolia

Morphological and molecular analyses in micropropagated berry plants acclimatized under ex vitro condition

2012 ◽  
Vol 92 (6) ◽  
pp. 1065-1073 ◽  
Author(s):  
S. C. Debnath ◽  
P. Vyas ◽  
J. C. Goyali ◽  
A. U. Igamberdiev
Keyword(s):  
Tissue Culture ◽  
Molecular Markers ◽  
Genetic Fidelity ◽  
Morphological Characters ◽  
Plant Production ◽  
Ex Vitro ◽  
In Vitro Morphogenesis ◽  
Molecular Analyses ◽  
Berry Crops

Debnath, S. C., Vyas, P., Goyali, J. C. and Igamberdiev, A. U. 2012. Morphological and molecular analyses in micropropagated berry plants acclimatized under ex vitro condition. Can. J. Plant Sci. 92: 1065–1073. Berry crops include, but are not limited to, the members of the genera Fragaria (strawberry; Rosaceae), Rubus (brambles: raspberry and blackberry; Rosaceae), Vaccinium (blueberry, cranberry and lingonberry; Ericaceae) and Ribes (currant and gooseberry; Grossulariaceae). While berry fruits have long enjoyed huge popularity among consumers, tremendous progress in plant tissue culture, resulting in great advances in micropropagation, has occurred. The in vitro morphogenesis seems to be highly dependent on plant growth regulators and media used for culture, which is again genotype specific. Although automation of micropropagation in bioreactors has been advanced as a possible way of reducing the cost of propagation, optimal plant production depends on better understanding of physiological and biochemical responses of plants to the signals of the culture microenvironment and an optimization of specific physical and chemical culture conditions to control the morphogenesis of berry plants in liquid culture systems. Increased branching, vigorous vegetative growth and change in biochemical components are often noted in micropropagated plants acclimatized under ex vitro condition. Clonal fidelity can be a serious problem and strategies have been developed to reduce the variation to manageable levels. Molecular markers have been introduced in tissue culture research and can potentially be used in various facets of pertinent studies with berry crops. This paper describes in depth the progress of various aspects of berry propagation in vitro, the characterization of micropropagated berry plants for morphological characters, and the employment of molecular markers in these plants for the assessment of genetic fidelity, uniformity, stability and trueness-to-type among donor plants and tissue culture regenerants.

Bioreactors and molecular analysis in berry crop micropropagation – A review

2011 ◽  
Vol 91 (1) ◽  
pp. 147-157 ◽  
Author(s):  
Samir Debnath
Keyword(s):  
Tissue Culture ◽  
Molecular Markers ◽  
Molecular Analysis ◽  
Genetic Fidelity ◽  
Culture Conditions ◽  
Plant Production ◽  
Solid Media ◽  
Berry Crops ◽  
Berry Fruits

Debnath, S. C. 2011. Bioreactors and molecular analysis in berry crop micropropagation – A review. Can. J. Plant Sci. 91: 147–157. While berry fruits have long enjoyed huge popularity among consumers, tremendous progress in plant tissue culture, resulting in great advances in micropropagation, has occurred. Of particular significance has been the evolution of the technology permitting multiplication of berry plants in bioreactors containing liquid media. Although automation of micropropagation in bioreactors has been advanced as a possible way of reducing propagation cost, optimal plant production depends upon better understanding of physiological and biochemical responses of plant to the signals of culture microenvironment and an optimization of specific physical and chemical culture conditions to control the morphogenesis of berry plants in liquid culture systems. Clonal fidelity can be a serious problem, and molecular strategies have been developed in order to reduce the variation to manageable levels. Molecular markers have been introduced to tissue culture research and can potentially be used in various facets of pertinent studies with berry crops. The paper focuses on bioreactor systems combined with semi-solid media used for in vitro culture of berry crops, cultivation of micropropagules and employment of molecular markers in micropropagated plants for the assessment of genetic fidelity, uniformity, stability and trueness-to-type among donor plants and tissue culture regenerants. The pertinent literature is reviewed and the relative merits and shortcomings of the various molecular markers applied are presented with an emphasis on the nature of tissue culture-induced variation.

scholarly journals Acute and long-term effects of tissue culture on contractile reactivity in renal arteries of the rat.

1989 ◽  
Vol 65 (4) ◽  
pp. 1125-1135 ◽  
Author(s):  
J G De Mey ◽  
M P Uitendaal ◽  
H C Boonen ◽  
M J Vrijdag ◽  
M J Daemen ◽  
...  
Keyword(s):  
Tissue Culture ◽  
Renal Arteries ◽  
Long Term Effects

Long-term monitoring of molecular markers can distinguish different seasonal patterns of fecal indicating bacteria sources

2015 ◽  
Vol 71 ◽  
pp. 227-243 ◽  
Author(s):  
Timothy E. Riedel ◽  
Vanessa Thulsiraj ◽  
Amity G. Zimmer-Faust ◽  
Rosi Dagit ◽  
Jenna Krug ◽  
...  
Keyword(s):  
Molecular Markers ◽  
Seasonal Patterns ◽  
Long Term Monitoring ◽  
Term Monitoring

Evaluation of Genetic Homogeneity in Tissue Culture Regenerates of Jatropha curcas L. using Flow Cytometer and DNA-based Molecular Markers

2013 ◽  
Vol 172 (1) ◽  
pp. 298-310 ◽  
Author(s):  
Mangal S. Rathore ◽  
P. Yadav ◽  
Shaik G. Mastan ◽  
Ch. R. Prakash ◽  
A. Singh ◽  
...  
Keyword(s):  
Tissue Culture ◽  
Molecular Markers ◽  
Jatropha Curcas ◽  
Flow Cytometer ◽  
Genetic Homogeneity ◽  
Jatropha Curcas L

EVALUATION OF GENETIC FIDELITY FOLLOWING LONG TERM CRYOPRESERVATION OF CONIFEROUS FOREST TREE SPECIES OF THE FAMILY PINACEAE

2014 ◽  
pp. 121-126
Author(s):  
H. Häggman ◽  
S. Sutela ◽  
J. Edesi ◽  
J. Krajňáková ◽  
A. Bertolini ◽  
...  
Keyword(s):  
Tree Species ◽  
Coniferous Forest ◽  
Genetic Fidelity ◽  
Forest Tree ◽  
Forest Tree Species ◽  
The Family

scholarly journals Laboratory Organization

1993 ◽  
Keyword(s):  
Tissue Culture ◽  
Dna Analysis ◽  
Simple Process ◽  
Cold Room ◽  
Chain Of Custody ◽  
Trade Shows ◽  
New Facility ◽  
Beta Counter ◽  
Dark Room

Laboratory organization involves both the physical establishment and its operation. It is perhaps simplest to divide the laboratory into its component sections and discuss each separately. The areas may physically overlap for a small facility, and depending on the operation specialty, some sections, such as tissue culture or probe amplification, may not be required. Unless the operation is large, dark room and cold room facilities and expensive equipment such as an ultracentrifuge and beta counter are best shared, if feasible. The setup of a DNA analysis facility is a relatively simple process if it is incorporated into an established biochemistry program; it is considerably more involved if no such base exists. The outline presented in this chapter is only a guide; individuals contemplating the development of a new facility should visit as many established centers as possible. Discussions with sales representatives and attendance at relevant trade shows and DNA conferences are invaluable. Office requirements for a DNA program are no different in principle from those of any other biochemistry program. At least one separate office is required, usually for the program director and, as space permits, offices for a clerk-secretary and senior technologist are useful. Everyone working in the laboratory must have at least a small partitioned desk space in a quiet location. Lockable fire-resistant cabinets are required to store sensitive records; these cabinets should be accessible, preferably located in the clerical area. Analysis results are worthless without proper documentation of a specimen’s chain of custody (continuity). Information, including time and conditions of specimen procurement, conditions of storage and shipment, date received by the laboratory, and reason for the analysis request is also required. These data can be manually recorded; however, entry into a computer program capable of sorting and maintaining records for long-term retrieval is almost mandatory. Storage of unprocessed specimens may be necessary, and if at all possible, DNA should be isolated when received.

LONG-TERM TISSUE CULTURE OF HUMAN SKIN

1956 ◽  
Vol 63 (1) ◽  
pp. 52-58
Author(s):  
VERNON P. PERRY ◽  
VIRGINIA J. EVANS ◽  
WILTON R. EARLE ◽  
GEORGE W. HYATT ◽  
WALLACE C. BEDELL
Keyword(s):  
Tissue Culture ◽  
Human Skin
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