Random chemical mutagenesis of a specific psbDI region coding for a lumenal loop of the D2 protein of photosystem II in Synechocystis sp. PCC 6803

1996 ◽  
Vol 30 (2) ◽  
pp. 243-254 ◽  
Author(s):  
Svetlana Ermakova-Gerdes ◽  
Sergey Shestakov ◽  
Wim Vermaas
Keyword(s):  
Photosystem Ii ◽  
Chemical Mutagenesis ◽  
Synechocystis Sp ◽  
D2 Protein ◽  
Pcc 6803 ◽  
Region Coding

scholarly journals A reversibly induced CRISPRi system targeting Photosystem II in the cyanobacterium Synechocystis sp. PCC 6803

2020 ◽  
Author(s):  
Deng Liu ◽  
Virginia M. Johnson ◽  
Himadri B. Pakrasi
Keyword(s):  
Photosystem Ii ◽  
Sole Carbon Source ◽  
Model Organism ◽  
Inducible Promoter ◽  
New Strategy ◽  
Photosynthetic Complexes ◽  
Promoter System ◽  
Synechocystis Sp ◽  
D2 Protein ◽  
Pcc 6803

ABSTRACTThe cyanobacterium Synechocystis sp. PCC 6803 is used as a model organism to study photosynthesis, as it can utilize glucose as the sole carbon source to support its growth under heterotrophic conditions. CRISPR interference (CRISPRi) has been widely applied to repress the transcription of genes in a targeted manner in cyanobacteria. However, a robust and reversible induced CRISPRi system has not been explored in Synechocystis 6803 to knock down and recover the expression of a targeted gene. In this study, we built a tightly controlled chimeric promoter, PrhaBAD-RSW, in which a theophylline responsive riboswitch was integrated into a rhamnose-inducible promoter system. We applied this promoter to drive the expression of ddCpf1 (DNase-dead Cpf1 nuclease) in a CRISPRi system and chose the PSII reaction center gene psbD (D2 protein) to target for repression. psbD was specifically knocked down by over 95% of its native expression, leading to severely inhibited Photosystem II activity and growth of Synechocystis 6803 under photoautotrophic conditions. Significantly, removal of the inducers rhamnose and theophylline reversed repression by CRISPRi. Expression of PsbD recovered following release of repression, coupled with increased Photosystem II content and activity. This reversibly induced CRISPRi system in Synechocystis 6803 represents a new strategy for study of the biogenesis of photosynthetic complexes in cyanobacteria.

Modified EPR spectra of the tyrosineD radical in photosystem II in site-directed mutants of Synechocystis sp. PCC 6803: Identification of side chains in the immediate vicinity of tyrosineD on the D2 protein

Biochemistry ◽  
1993 ◽  
Vol 32 (20) ◽  
pp. 5436-5441 ◽  
Author(s):  
Cecilia Tommos ◽  
Lars Davidsson ◽  
Bengt Svensson ◽  
Cathy Madsen ◽  
Wim Vermaas ◽  
...  
Keyword(s):  
Photosystem Ii ◽  
Epr Spectra ◽  
Side Chains ◽  
Synechocystis Sp ◽  
D2 Protein ◽  
Pcc 6803

scholarly journals Cloning of the psbK gene from Synechocystis sp. PCC 6803 and characterization of photosystem II in mutants lacking PSII-K

1991 ◽  
Vol 266 (17) ◽  
pp. 11111-11115
Author(s):  
M. Ikeuchi ◽  
B. Eggers ◽  
G.Z. Shen ◽  
A. Webber ◽  
J.J. Yu ◽  
...  
Keyword(s):  
Photosystem Ii ◽  
Synechocystis Sp ◽  
Pcc 6803
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