Evaluation of a DNA probe for the quantitative detection of common bacterial blight in common bean and its application in a breeding program

Euphytica ◽  
1996 ◽  
Vol 90 (2) ◽  
pp. 129-135 ◽  
Author(s):  
E. Caroline Constabel ◽  
Thomas E. Michaels ◽  
Paul H. Goodwin ◽  
Jorge E. Mayer ◽  
Marclal A. Pastor Corrales
Keyword(s):  
Common Bean ◽  
Bacterial Blight ◽  
Dna Probe ◽  
Breeding Program ◽  
Quantitative Detection ◽  
Common Bacterial Blight

Mapping of Common Bacterial Blight Resistance Gene in Common Bean

2017 ◽  
Vol 43 (1) ◽  
pp. 1
Author(s):  
Ji-Feng ZHU ◽  
Jing WU ◽  
Lan-Fen WANG ◽  
Zhen-Dong ZHU ◽  
Shu-Min WANG
Keyword(s):  
Common Bean ◽  
Resistance Gene ◽  
Bacterial Blight ◽  
Blight Resistance ◽  
Common Bacterial Blight ◽  
Bacterial Blight Resistance ◽  
Bacterial Blight Resistance Gene

Direct and Marker-Assisted Selection for Resistance to Common Bacterial Blight in Common Bean

Crop Science ◽  
2012 ◽  
Vol 52 (4) ◽  
pp. 1511-1521 ◽  
Author(s):  
Robert W. Duncan ◽  
Robert L. Gilbertson ◽  
Shree P. Singh
Keyword(s):  
Common Bean ◽  
Marker Assisted Selection ◽  
Bacterial Blight ◽  
Common Bacterial Blight ◽  
Selection For

Comparison of Seed Transmission and Survival of Xanthomonas axonopodis pv. phaseoli and Xanthomonas fuscans subsp. fuscans in Common Bean Seeds

2013 ◽  
Vol 14 (1) ◽  
pp. 41 ◽  
Author(s):  
Y. He ◽  
G. P. Munkvold
Keyword(s):  
Phaseolus Vulgaris ◽  
Common Bean ◽  
Bacterial Blight ◽  
Disease Incidence ◽  
Seed Transmission ◽  
Common Bacterial Blight ◽  
Phaseolus Vulgaris L ◽  
Transmission Frequency ◽  
Xanthomonas Axonopodis ◽  
Seed Health

Xanthomonas axonopodis pv. phaseoli (Smith) Vauterin (Xap) and Xanthomonas fuscans subsp. fuscans Schaad et al. (Xff) cause indistinguishable symptoms known as common bacterial blight of bean (Phaseolus vulgaris L.). These results confirm a higher disease incidence and seed transmission frequency of Xff compared to Xap and reinforce the need for seed health tests that can differentiate the two species. Accepted for publication 21 August 2013. Published 23 September 2013.

scholarly journals Molecular-marker-based Genetic Analysis of Tepary Bean-derived Common Bacterial Blight Resistance in Different Developmental Stages of Common Bean

1997 ◽  
Vol 122 (3) ◽  
pp. 329-337 ◽  
Author(s):  
Geunhwa Jung ◽  
Paul W. Skroch ◽  
Dermot P. Coyne ◽  
James Nienhuis ◽  
E. Arnaud-Santana ◽  
...  
Keyword(s):  
Linkage Group ◽  
Common Bean ◽  
Linkage Map ◽  
Recombinant Inbred ◽  
Bacterial Blight ◽  
Chromosomal Region ◽  
Bacterial Disease ◽  
Common Bacterial Blight ◽  
Group 5 ◽  
Group 1

Randomly amplified polymorphic DNA (RAPD) molecular markers were used to construct a partial genetic linkage map in a recombinant inbred population derived from the common bean (Phaseolus vulgaris L.) cross PC-50 × XAN-159 for studying the genetics of bacterial disease resistance in common bean. The linkage map spanned 426 cM and included 168 RAPD markers and 2 classical markers with 11 unassigned markers. The seventy recombinant inbred lines were evaluated for resistance to two strains of common bacterial blight [Xanthomonas campestris pv. phaseoli (Smith) Dye] (Xcp). Common bacterial blight (CBB) resistance was evaluated for Xcp strain EK-11 in later-developed trifoliolate leaves and for Xcp strains, DR-7 and EK-11, in first trifoliolate leaves, seeds, and pods. One to four quantitative trait loci (QTLs) accounted for 18% to 53% of the phenotypic variation for traits. Most significant effects for CBB resistance were associated with one chromosomal region on linkage group 5 and with two regions on linkage group 1, of the partial linkage map. The chromosomal region (a 13-cM interval) in linkage group 5 was significantly associated with resistance to Xcp strains DR-7 and EK-11 in leaves, pods, and seeds. The regions in linkage group 1 were also significantly associated with resistance to both Xcp strains in more than one plant organ. In addition, a seedcoat pattern gene (C) and a flower color gene (vlae) were mapped in linkage groups 1 and 5, respectively, of the partial linkage map. The V locus was found to be linked to a QTL with a major effect on CBB resistance.

Confirmation of QTL Associated with Common Bacterial Blight Resistance in Four Different Genetic Backgrounds in Common Bean

Crop Science ◽  
1999 ◽  
Vol 39 (5) ◽  
pp. 1448-1455 ◽  
Author(s):  
G. Jung ◽  
P. W. Skroch ◽  
J. Nienhuis ◽  
D. P. Coyne ◽  
E. Arnaud-Santana ◽  
...  
Keyword(s):  
Common Bean ◽  
Bacterial Blight ◽  
Blight Resistance ◽  
Common Bacterial Blight ◽  
Bacterial Blight Resistance

Mapping genetic factors affecting the reaction to Xanthomonas axonopodis pv. phaseoli in Phaseolus vulgaris L. under field conditions

Genome ◽  
2001 ◽  
Vol 44 (6) ◽  
pp. 1046-1056 ◽  
Author(s):  
B Tar'an ◽  
T E Michaels ◽  
K P Pauls
Keyword(s):  
Phaseolus Vulgaris ◽  
Common Bean ◽  
Length Polymorphism ◽  
Bacterial Blight ◽  
Genetic Factors ◽  
Fragment Length Polymorphism ◽  
Segregation Ratio ◽  
Common Bacterial Blight ◽  
Phaseolus Vulgaris L ◽  
Xanthomonas Axonopodis

The objectives of the present study were to evaluate the field effects of Xanthomonas axonopodis pv. phaseoli (Xap), which causes common bacterial blight (CBB) on common bean (Phaseolus vulgaris L.), and to identify genetic factors for resistance to CBB using a linkage map constructed with random amplified polymorphic DNA (RAPD), restriction fragment length polymorphism (RFLP), simple sequence repeat (SSR), and amplified fragment length polymorphism (AFLP) markers. One hundred and forty-two F2:4 lines, derived from a cross between 'OAC Seaforth' and 'OAC 95-4', and the parents were evaluated for their field reaction to CBB. In the inoculated plots, the reaction to CBB was negatively correlated with seed yield, days to maturity, plant height, hypocotyl diameter, pods per plant, and harvest index. A reduction in seed yield and its components was observed when disease-free and CBB-inoculated plots were compared. The broad-sense heritability estimate of the reaction to CBB was 0.74. The disease segregation ratio was not significantly different from the expected segregation ratio for a single locus in an F2 generation. The major gene for CBB resistance was localized on linkage group (LG) G5. A simple interval mapping procedure identified three genomic regions associated with the reaction to CBB. One quantitative trait loci (QTL), each on LG G2 (BNG71DraI), G3 (BNG21EcoRV), and G5 (PHVPVPK-1) explained 36.3%, 10.2%, and 42.2% of the phenotypic variation for the reaction to CBB, respectively. Together, these loci explained 68.4% of the phenotypic variation. The relative positions of these QTL on the core common bean map and their comparison with the previous QTL for CBB resistance are discussed.Key words: common bean, molecular markers, common bacterial blight.

scholarly journals Application of Image Analysis in Studies of Quantitative Disease Resistance, Exemplified Using Common Bacterial Blight–Common Bean Pathosystem

2012 ◽  
Vol 102 (4) ◽  
pp. 434-442 ◽  
Author(s):  
Weilong Xie ◽  
Kangfu Yu ◽  
K. Peter Pauls ◽  
Alireza Navabi
Keyword(s):  
Image Analysis ◽  
Common Bean ◽  
Disease Severity ◽  
Bacterial Blight ◽  
Visual Assessment ◽  
Common Bacterial Blight ◽  
Genetic Studies ◽  
Visual Scale ◽  
Quantitative Genetic ◽  
Visual Assessments

The effectiveness of image analysis (IA) compared with an ordinal visual scale, for quantitative measurement of disease severity, its application in quantitative genetic studies, and its effect on the estimates of genetic parameters were investigated. Studies were performed using eight backcross-derived families of common bean (Phaseolus vulgaris) (n = 172) segregating for the molecular marker SU91, known to be associated with a quantitative trait locus (QTL) for resistance to common bacterial blight (CBB), caused by Xanthomonas campestris pv. phaseoli and X. fuscans subsp. fuscans. Even though both IA and visual assessments were highly repeatable, IA was more sensitive in detecting quantitative differences between bean genotypes. The CBB phenotypic difference between the two SU91 genotypic groups was consistently more than fivefold for IA assessments but generally only two- to threefold for visual assessments. Results suggest that the visual assessment results in overestimation of the effect of QTL in genetic studies. This may have been caused by lack of additivity and uneven intervals of the visual scale. Although visual assessment of disease severity is a useful tool for general selection in breeding programs, assessments using IA may be more suitable for phenotypic evaluations in quantitative genetic studies involving CBB resistance as well as other foliar diseases.

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