Direct and Marker-Assisted Selection for Resistance to Common Bacterial Blight in Common Bean

Crop Science ◽  
2012 ◽  
Vol 52 (4) ◽  
pp. 1511-1521 ◽  
Author(s):  
Robert W. Duncan ◽  
Robert L. Gilbertson ◽  
Shree P. Singh
Keyword(s):  
Common Bean ◽  
Marker Assisted Selection ◽  
Bacterial Blight ◽  
Common Bacterial Blight ◽  
Selection For

scholarly journals Use of Marker-assisted Selection to Breed for Resistance to Common Bacterial Blight in Common Bean

2007 ◽  
Vol 132 (3) ◽  
pp. 381-386 ◽  
Author(s):  
Patrick D. O'Boyle ◽  
James D. Kelly ◽  
William W. Kirk
Keyword(s):  
Common Bean ◽  
Marker Assisted Selection ◽  
Bacterial Blight ◽  
Field Experiments ◽  
Selection Process ◽  
Bacterial Pathogen ◽  
Common Bacterial Blight ◽  
Epistatic Interactions ◽  
Sequence Characterized Amplified Region ◽  
Selection Of

Common bacterial blight (CBB), incited by the bacterial pathogen Xanthomonas axonopodis pv. phaseoli (Smith) Vauterin et al., is one of the most serious seed-borne diseases of common bean (Phaseolus vulgaris L.) worldwide. Although previous attempts to breed CBB-resistant bean cultivars have had limited success due to the quantitative nature of resistance, progress in marker-assisted selection has created new opportunities for breeders to improve the selection process. The potential of independent linked markers for indirect selection of CBB resistance was evaluated in field experiments in East Lansing and Saginaw, Mich. The presence of the SU91 marker was correlated with lower CBB leaf scores in East Lansing (r = −0.50***) and Saginaw (r = −0.59***) and correlated with pod resistance in Saginaw (r = −0.48***). SU91 exhibited a slight negative correlation with yield (r = −0.20*) in East Lansing but showed no association with yield in Saginaw. Plant selections carrying SU91 were crossed with a different source of CBB resistance linked to the sequence-characterized amplified region marker BC420 on bean linkage group B6. The effects of SU91 and BC420 markers were examined in two greenhouse studies. The presence of SU91 was correlated with lower CBB disease ratings for leaves (r = −0.20*) and pods (r = −0.27***). Presence of BC420 was only correlated with low pod ratings (r = −0.19*) in one experiment and CBB leaf resistance (r = −0.18*) in the second experiment. Presence of both markers resulted in lower levels of CBB resistance than provided by either marker alone, possibly indicating epistatic interactions between the independent loci conditioning CBB resistance in common bean.

Mapping of Common Bacterial Blight Resistance Gene in Common Bean

2017 ◽  
Vol 43 (1) ◽  
pp. 1
Author(s):  
Ji-Feng ZHU ◽  
Jing WU ◽  
Lan-Fen WANG ◽  
Zhen-Dong ZHU ◽  
Shu-Min WANG
Keyword(s):  
Common Bean ◽  
Resistance Gene ◽  
Bacterial Blight ◽  
Blight Resistance ◽  
Common Bacterial Blight ◽  
Bacterial Blight Resistance ◽  
Bacterial Blight Resistance Gene

scholarly journals Marker Assisted Selection for Common Bean Diseases Improvements in Tanzania: Prospects and Future Needs

10.5772/52823 ◽  
2013 ◽  
Author(s):  
George Muhamba ◽  
Luseko Amos ◽  
Deogracious Protas ◽  
Paul Mbogo ◽  
Susan Nchimbi-Msoll
Keyword(s):  
Common Bean ◽  
Marker Assisted Selection ◽  
Future Needs ◽  
Selection For

Comparison of Seed Transmission and Survival of Xanthomonas axonopodis pv. phaseoli and Xanthomonas fuscans subsp. fuscans in Common Bean Seeds

2013 ◽  
Vol 14 (1) ◽  
pp. 41 ◽  
Author(s):  
Y. He ◽  
G. P. Munkvold
Keyword(s):  
Phaseolus Vulgaris ◽  
Common Bean ◽  
Bacterial Blight ◽  
Disease Incidence ◽  
Seed Transmission ◽  
Common Bacterial Blight ◽  
Phaseolus Vulgaris L ◽  
Transmission Frequency ◽  
Xanthomonas Axonopodis ◽  
Seed Health

Xanthomonas axonopodis pv. phaseoli (Smith) Vauterin (Xap) and Xanthomonas fuscans subsp. fuscans Schaad et al. (Xff) cause indistinguishable symptoms known as common bacterial blight of bean (Phaseolus vulgaris L.). These results confirm a higher disease incidence and seed transmission frequency of Xff compared to Xap and reinforce the need for seed health tests that can differentiate the two species. Accepted for publication 21 August 2013. Published 23 September 2013.

scholarly journals Molecular-marker-based Genetic Analysis of Tepary Bean-derived Common Bacterial Blight Resistance in Different Developmental Stages of Common Bean

1997 ◽  
Vol 122 (3) ◽  
pp. 329-337 ◽  
Author(s):  
Geunhwa Jung ◽  
Paul W. Skroch ◽  
Dermot P. Coyne ◽  
James Nienhuis ◽  
E. Arnaud-Santana ◽  
...  
Keyword(s):  
Linkage Group ◽  
Common Bean ◽  
Linkage Map ◽  
Recombinant Inbred ◽  
Bacterial Blight ◽  
Chromosomal Region ◽  
Bacterial Disease ◽  
Common Bacterial Blight ◽  
Group 5 ◽  
Group 1

Randomly amplified polymorphic DNA (RAPD) molecular markers were used to construct a partial genetic linkage map in a recombinant inbred population derived from the common bean (Phaseolus vulgaris L.) cross PC-50 × XAN-159 for studying the genetics of bacterial disease resistance in common bean. The linkage map spanned 426 cM and included 168 RAPD markers and 2 classical markers with 11 unassigned markers. The seventy recombinant inbred lines were evaluated for resistance to two strains of common bacterial blight [Xanthomonas campestris pv. phaseoli (Smith) Dye] (Xcp). Common bacterial blight (CBB) resistance was evaluated for Xcp strain EK-11 in later-developed trifoliolate leaves and for Xcp strains, DR-7 and EK-11, in first trifoliolate leaves, seeds, and pods. One to four quantitative trait loci (QTLs) accounted for 18% to 53% of the phenotypic variation for traits. Most significant effects for CBB resistance were associated with one chromosomal region on linkage group 5 and with two regions on linkage group 1, of the partial linkage map. The chromosomal region (a 13-cM interval) in linkage group 5 was significantly associated with resistance to Xcp strains DR-7 and EK-11 in leaves, pods, and seeds. The regions in linkage group 1 were also significantly associated with resistance to both Xcp strains in more than one plant organ. In addition, a seedcoat pattern gene (C) and a flower color gene (vlae) were mapped in linkage groups 1 and 5, respectively, of the partial linkage map. The V locus was found to be linked to a QTL with a major effect on CBB resistance.

Confirmation of QTL Associated with Common Bacterial Blight Resistance in Four Different Genetic Backgrounds in Common Bean

Crop Science ◽  
1999 ◽  
Vol 39 (5) ◽  
pp. 1448-1455 ◽  
Author(s):  
G. Jung ◽  
P. W. Skroch ◽  
J. Nienhuis ◽  
D. P. Coyne ◽  
E. Arnaud-Santana ◽  
...  
Keyword(s):  
Common Bean ◽  
Bacterial Blight ◽  
Blight Resistance ◽  
Common Bacterial Blight ◽  
Bacterial Blight Resistance

Mapping genetic factors affecting the reaction to Xanthomonas axonopodis pv. phaseoli in Phaseolus vulgaris L. under field conditions

Genome ◽  
2001 ◽  
Vol 44 (6) ◽  
pp. 1046-1056 ◽  
Author(s):  
B Tar'an ◽  
T E Michaels ◽  
K P Pauls
Keyword(s):  
Phaseolus Vulgaris ◽  
Common Bean ◽  
Length Polymorphism ◽  
Bacterial Blight ◽  
Genetic Factors ◽  
Fragment Length Polymorphism ◽  
Segregation Ratio ◽  
Common Bacterial Blight ◽  
Phaseolus Vulgaris L ◽  
Xanthomonas Axonopodis

The objectives of the present study were to evaluate the field effects of Xanthomonas axonopodis pv. phaseoli (Xap), which causes common bacterial blight (CBB) on common bean (Phaseolus vulgaris L.), and to identify genetic factors for resistance to CBB using a linkage map constructed with random amplified polymorphic DNA (RAPD), restriction fragment length polymorphism (RFLP), simple sequence repeat (SSR), and amplified fragment length polymorphism (AFLP) markers. One hundred and forty-two F2:4 lines, derived from a cross between 'OAC Seaforth' and 'OAC 95-4', and the parents were evaluated for their field reaction to CBB. In the inoculated plots, the reaction to CBB was negatively correlated with seed yield, days to maturity, plant height, hypocotyl diameter, pods per plant, and harvest index. A reduction in seed yield and its components was observed when disease-free and CBB-inoculated plots were compared. The broad-sense heritability estimate of the reaction to CBB was 0.74. The disease segregation ratio was not significantly different from the expected segregation ratio for a single locus in an F2 generation. The major gene for CBB resistance was localized on linkage group (LG) G5. A simple interval mapping procedure identified three genomic regions associated with the reaction to CBB. One quantitative trait loci (QTL), each on LG G2 (BNG71DraI), G3 (BNG21EcoRV), and G5 (PHVPVPK-1) explained 36.3%, 10.2%, and 42.2% of the phenotypic variation for the reaction to CBB, respectively. Together, these loci explained 68.4% of the phenotypic variation. The relative positions of these QTL on the core common bean map and their comparison with the previous QTL for CBB resistance are discussed.Key words: common bean, molecular markers, common bacterial blight.

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