Effects of nucleus, cytoplasm and male sterile nucleus-cytoplasm combinations on callus initiation in anther culture of wheat

Euphytica ◽  
1989 ◽  
Vol 44 (3) ◽  
pp. 235-240 ◽  
Author(s):  
P. W. Becraft ◽  
G. A. Taylor
Keyword(s):  
Anther Culture ◽  
Male Sterile ◽  
Callus Initiation

Anther culture of male-sterile tomato (Lycopersicon esculentum mill.) mutants

1980 ◽  
Vol 17 (3) ◽  
pp. 353-361 ◽  
Author(s):  
Daniel Zamir ◽  
Richard A. Jones ◽  
Nachum Kedar
Keyword(s):  
Lycopersicon Esculentum ◽  
Anther Culture ◽  
Male Sterile ◽  
Lycopersicon Esculentum Mill

scholarly journals Maintenance of male sterile germplasm in Brassica rapa by in vitro propagation

2000 ◽  
Vol 9 (3) ◽  
pp. 231-238 ◽  
Author(s):  
Y.-D. GUO ◽  
T. NIEMELÄ ◽  
U. TULISALO
Keyword(s):  
Brassica Rapa ◽  
Shoot Formation ◽  
Dichlorophenoxyacetic Acid ◽  
Male Sterile ◽  
Flower Buds ◽  
Breeding Programs ◽  
Regenerated Plants ◽  
Callus Initiation ◽  
2,4 Dichlorophenoxyacetic Acid

An efficient tissue culture system for plant regeneration, from mature cut branches, was established to maintain male sterile material in Brassica rapa L. The new-growth immature pods from the cut branches were used as explantsresults in callus initiation (37 calli from 25 explants) and shoot formation (17 shoots from 75 explants) than flower buds and branch stems. Auxin [2,4-dichlorophenoxyacetic acid (2,4-D), 2 to 5 mg l-1] and cytokinin [6-benzylaminopurine (BA), 2 to 4 mg l-1] were essential in callus and shoot formation, respectively. Callus initiation and shoot regeneration capacities were genotype dependent. The regenerated plants were male sterile and were used in breeding programs.;

Rice anther culture: callus initiation and androclonal variation in progenies of regenerated plants

1995 ◽  
Vol 14 (7) ◽  
Author(s):  
P. Sathish ◽  
OlufL. Gamborg ◽  
MurrayW. Nabors
Keyword(s):  
Anther Culture ◽  
Regenerated Plants ◽  
Callus Initiation

scholarly journals Kultur Antera untuk Percepatan Perakitan Varietas Padi di Indonesia

2016 ◽  
Vol 8 (2) ◽  
pp. 78 ◽  
Author(s):  
Iswari S Dewi ◽  
Bambang S. Purwoko
Keyword(s):  
Anther Culture ◽  
Aluminum Toxicity ◽  
Selection Process ◽  
Indica Rice ◽  
Doubled Haploids ◽  
Population Increase ◽  
Rice Breeding ◽  
Male Sterile ◽  
Breeding Method ◽  
Biotic Stresses

<p>Rice is a staple<br />food crop in Indonesia, while the need is increasing due to<br />high rice consumption as well as population increase. The<br />problems can be solved through increase of national rice<br />production. Productivity of lowland and upland should be<br />increased intensively and other potential dry area outside<br />Java and Bali Islands should be considered for extending the<br />area of production. Recently, high yielding variety such as<br />semi dwarf variety, hybrid rice, and new plant type of rice<br />were being developed by Indonesian breeders. However,<br />new method is needed to complement conventional<br />breeding method in order to accelerate rice breeding.<br />Anther culture is one of in vitro culture techniques that can<br />be used to accelerate the obtainment of pure lines through<br />doubled-haploids (DHs) regenerated at first generation of<br />culture for less than one year. Thus, application of anther<br />culture in conventional breeding will increase the efficiency<br />of selection process as well as reducing the cost for labour,<br />land and breeder’s time. The obtainment of green plantlets<br />derived from anther culture of indica rice subspecies has<br />been improved by the addition of 1 mM putrescine into<br />induction and regeneration media. Recently, several upland<br />rice lines tolerant to abiotic stresses (i.e. low light intensity<br />and aluminum toxicity) and biotic stresses (i.e. leaf and<br />neck blast), several lowland rice/paddy lines tolerant to<br />biotic stresses (i.e. bacterial leaf blight and blast), and<br />several hybrid parental lines (i.e. male sterile, maintainer<br />and restorer) were obtained in 2-3 years from several rice<br />breeding program involving anther culture. However,<br />potential use anther culture to provide unique genetic<br />material for mapping populations for use in functional<br />genomics and molecular breeding has not been explored.<br />The results indicated that anther culture is a feasible<br />technology that can be used for accelerating rice breeding<br />program in Indonesia.</p>

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