Rice anther culture: callus initiation and androclonal variation in progenies of regenerated plants

1995 ◽  
Vol 14 (7) ◽  
Author(s):  
P. Sathish ◽  
OlufL. Gamborg ◽  
MurrayW. Nabors
Keyword(s):  
Anther Culture ◽  
Regenerated Plants ◽  
Callus Initiation

Anther culture of a 2n pollen producing clone of Solanum phureja Juz. &Buk.

1985 ◽  
Vol 27 (5) ◽  
pp. 559-564 ◽  
Author(s):  
R. E. Veilleux ◽  
J. Booze-Daniels ◽  
E. Pehu
Keyword(s):  
Anther Culture ◽  
Polyacrylamide Gel Electrophoresis ◽  
Pollen Grains ◽  
Unreduced Gametes ◽  
Solanum Phureja ◽  
2N Pollen ◽  
Regenerated Plants ◽  
Series Of Experiments ◽  
Pollen Formation ◽  
First And Second Generation

In a series of experiments, a total of 95 plants were regenerated from culture of 1416 anthers of a single genotype (PP5) of Solanum phureja that expressed a variable frequency of 2n pollen by the genetic equivalent of first division restitution. The regenerated plants included 29 monoploids (2n = x = 12), 58 diploids (2n = 2x = 24) and 8 tetraploids (2n = 4x = 48). Monoploids carrying the potential for 2n pollen formation are central to the development of a breeding scheme to construct highly heterozygous diplandroids. Segregation of tuber flesh color and tuber protein bands revealed by polyacrylamide gel electrophoresis in first and second generation anther-derived diploid plants provided evidence for both embryogenesis of 2n pollen as well as doubling of monoploid genomes as sources of anther-derived diploids. Because of variation for the same genetic markers in anther-derived tetraploids, sources other than embryogenesis of doubly restituted (4n) pollen grains were implied. An enhanced response to anther culture was noted in some anther-derived dihaploids.Key words: unreduced gametes, potato, anther culture, diplandroid, monoploid.

scholarly journals Somatic embryogenesis from in vitro anther culture of apomictic buffel grass genotypes and analysis of regenerated plants using flow cytometry

2014 ◽  
Vol 117 (3) ◽  
pp. 311-322 ◽  
Author(s):  
Edgardo Carloni ◽  
Andrea Ribotta ◽  
Eliana López Colomba ◽  
Sabrina Griffa ◽  
Mariana Quiroga ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Somatic Embryogenesis ◽  
Anther Culture ◽  
Buffel Grass ◽  
Regenerated Plants

Plant regeneration and chromosome instability in tissue culture of Elymus canadensis × E. trachycaulus F1 hybrid

Genome ◽  
1992 ◽  
Vol 35 (1) ◽  
pp. 88-91 ◽  
Author(s):  
P. S. Kumar ◽  
P. D. Walton
Keyword(s):  
Tissue Culture ◽  
Plant Regeneration ◽  
Chromosome Number ◽  
Chromosome Instability ◽  
Ms Medium ◽  
Immature Inflorescence ◽  
F1 Hybrid ◽  
Regenerated Plants ◽  
Callus Initiation ◽  
Elymus Canadensis

Callus was induced from pieces of immature inflorescence of a sterile F1 hybrid (2n = 4x = 28) between Elymus canadensis and E. trachycaulus on MS medium supplemented with 2 mg/L of 2,4-D. Calli were of two types: compact or soft. Regeneration occurred predominantly from the compact callus. Although some plantlets were obtained on the callus initiation medium, the frequency of regeneration increased considerably when the callus was transferred to an auxin-free MS medium. Deliberate aging of callus induced cytological instability and variation in chromosome number of the regenerants. Five of the 43 regenerated plants had deviant chromosome numbers, including an octoploid (2n = 8x = 56). Chromosome pairing in the octoploid plant suggests that it originated through chromosome doubling.Key words: callus culture, variation, chromosome number.

scholarly journals Maintenance of male sterile germplasm in Brassica rapa by in vitro propagation

2000 ◽  
Vol 9 (3) ◽  
pp. 231-238 ◽  
Author(s):  
Y.-D. GUO ◽  
T. NIEMELÄ ◽  
U. TULISALO
Keyword(s):  
Brassica Rapa ◽  
Shoot Formation ◽  
Dichlorophenoxyacetic Acid ◽  
Male Sterile ◽  
Flower Buds ◽  
Breeding Programs ◽  
Regenerated Plants ◽  
Callus Initiation ◽  
2,4 Dichlorophenoxyacetic Acid

An efficient tissue culture system for plant regeneration, from mature cut branches, was established to maintain male sterile material in Brassica rapa L. The new-growth immature pods from the cut branches were used as explantsresults in callus initiation (37 calli from 25 explants) and shoot formation (17 shoots from 75 explants) than flower buds and branch stems. Auxin [2,4-dichlorophenoxyacetic acid (2,4-D), 2 to 5 mg l-1] and cytokinin [6-benzylaminopurine (BA), 2 to 4 mg l-1] were essential in callus and shoot formation, respectively. Callus initiation and shoot regeneration capacities were genotype dependent. The regenerated plants were male sterile and were used in breeding programs.;

scholarly journals Methods of Anther Culture of Pearl Millet and Ploidy Level of Regenerated Plants.

2000 ◽  
Vol 69 (2) ◽  
pp. 224-228
Author(s):  
Akiko SHIGEMUNE ◽  
Tomohiko YOSHIDA
Keyword(s):  
Anther Culture ◽  
Pearl Millet ◽  
Ploidy Level ◽  
Regenerated Plants

scholarly journals Production of New Regenerated Plants by Anther Culture of the Hybrids of Italian ryegrass X Tall fescue

2002 ◽  
Vol 22 (4) ◽  
pp. 273-278 ◽  
Author(s):  
K.Y. Kim ◽  
K.M. Kang ◽  
K.J. Choi ◽  
Y.S. Jang ◽  
Y.C. Lim ◽  
...  
Keyword(s):  
Anther Culture ◽  
Tall Fescue ◽  
Italian Ryegrass ◽  
Regenerated Plants

scholarly journals Time of In Vitro Anther Culture May Moderate Action of Copper and Silver Ions that Affect the Relationship between DNA Methylation Change and the Yield of Barley Green Regenerants

Plants ◽  
2020 ◽  
Vol 9 (9) ◽  
pp. 1064
Author(s):  
Piotr T. Bednarek ◽  
Renata Orłowska
Keyword(s):  
Anther Culture ◽  
De Novo ◽  
Silver Ions ◽  
Dna Demethylation ◽  
Induction Medium ◽  
Green Plants ◽  
Ion Concentrations ◽  
Regenerated Plants ◽  
Short Time

Plant anther culture allows for the regeneration of uniform and homozygous double haploids. However, off-type regenerants may appear as a result of so-called tissue culture-induced variation (TCIV). In addition, the presence of Cu2+ and Ag+ ions in the culture medium might influence the number of green plants. The regenerants were obtained via anther cultures of barley under varying Cu2+ and Ag+ ion concentrations in the induction medium during distinct time conditions. DArTseqMet markers were evaluated based on regenerants and donor plants and delivering data on DNA demethylation (DM) and de novo methylation (DNM) and changes in methylation (Delta). The number of green regenerated plants per 100 anthers (GPs) was evaluated. The Cu2+ and Ag+ ion concentrations moderated relationships between Delta and the number of green plants conditional on time of tissue cultures. Depending on the ions, moderated moderation is valid within the different time of anther culture. When the highest concentration of copper is analyzed, plant regeneration is possible under short ‘Time’ (21 days) of anther culture wherein Delta is negative or under elongated Time when Delta is positive. Under 21 days of culture, the highest concentration of silver ions and when Delta is negative, some regenerants could be evaluated. However, under high Ag+ concentration when Time of culture is long and Delta positive, the highest number of green plants could be obtained.

scholarly journals Successful Regeneration and Characterization of Anther Derived Rice Hybrid Plants from O. Sativa L. X O. Rufipogon Griff.

1970 ◽  
Vol 5 (5) ◽  
pp. 14-18 ◽  
Author(s):  
Bindeshwar Prasad Sah ◽  
Raj Kumar Niroula
Keyword(s):  
Anther Culture ◽  
Doubled Haploid ◽  
Doubled Haploid Population ◽  
Albino Plants ◽  
Large Size ◽  
Hybrid Plants ◽  
Regenerated Plants ◽  
Scientific World ◽  
Haploid Population ◽  
Doubled Haploid Plants

Doubled haploid regenerated through anther culture of wide cross rice hybrids is one of the potential tools to produce various useful genetic and cytogenetic stocks for molecular studies. In this study, an interspecific hybrid derived from Oryza sativa and O. rufipogon was subjected to anther culture with the aim to regenerate doubled haploid population for their characterization. A total of 21 green and 31 albino plants were obtained from 1904 anthers of O. sativa cv. Himali x O. rufipogon. Six types of androclonal variants were identified based on morphology and panicle characteristics of regenerated plants. Among 21 plants, only six were found diploid with partially fertility. Despite the sterility and preponderance of wild traits, some of the plants showed useful traits such as increased in panicle and spikelet length, reduced shattering and awning, and large size anther as in O. rufipogon. Two of the regenerants were superior for panicle related traits over either parent. The genotyping study using SSR markers also revealed the substantial variation among regenerants. At least three allelic variants were recorded among individual plants based on our preliminary study. The regenerated doubled haploid plants showed large amount of useful variation both at phenotype and molecular level and they can be exploited to enrich the current rice gene pool. Key words: Anther culturability; Albino; Androclon; Calli; Meiosis; SSR marker. DOI: 10.3126/sw.v5i5.2649 Scientific World, Vol. 5, No. 5, July 2007 14-18

scholarly journals Meiosis of anther culture regenerants in asparagus (Asparagus officinalis L.)

1998 ◽  
Vol 21 (1) ◽  
pp. 93-97 ◽  
Author(s):  
Leonardo Galli ◽  
Judith Viégas ◽  
Eliane Augustin ◽  
Marcia Ines Eckert ◽  
João Baptista da Silva
Keyword(s):  
Anther Culture ◽  
Genomic Instability ◽  
Chromosomal Abnormalities ◽  
Asparagus Officinalis ◽  
Pollen Mother Cells ◽  
Regenerated Plants ◽  
Mother Cells

Pollen mother cells obtained from regenerated plants of asparagus (Asparagus officinalis L.), in a population composed exclusively of male plants, through the process of anther culture from the hybrid G27 X 22-8, were analyzed during meiosis. It was observed that, during theprocess of anther culture by organogenesis, the pollen mother cells of the regenerants had great genomic instability, as evidenced by disturbances in all the meiotic phases of the first and second division. Furthermore, structural chromosomal abnormalities, in addition to aneuploidy and polyploidy, were observed.

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