scholarly journals Maintenance of male sterile germplasm in Brassica rapa by in vitro propagation

2000 ◽  
Vol 9 (3) ◽  
pp. 231-238 ◽  
Author(s):  
Y.-D. GUO ◽  
T. NIEMELÄ ◽  
U. TULISALO
Keyword(s):  
Brassica Rapa ◽  
Shoot Formation ◽  
Dichlorophenoxyacetic Acid ◽  
Male Sterile ◽  
Flower Buds ◽  
Breeding Programs ◽  
Regenerated Plants ◽  
Callus Initiation ◽  
2,4 Dichlorophenoxyacetic Acid

An efficient tissue culture system for plant regeneration, from mature cut branches, was established to maintain male sterile material in Brassica rapa L. The new-growth immature pods from the cut branches were used as explantsresults in callus initiation (37 calli from 25 explants) and shoot formation (17 shoots from 75 explants) than flower buds and branch stems. Auxin [2,4-dichlorophenoxyacetic acid (2,4-D), 2 to 5 mg l-1] and cytokinin [6-benzylaminopurine (BA), 2 to 4 mg l-1] were essential in callus and shoot formation, respectively. Callus initiation and shoot regeneration capacities were genotype dependent. The regenerated plants were male sterile and were used in breeding programs.;

scholarly journals Regeneration of Ornamental Cherry (Prunus) Taxa from Mature Stored Seed

HortScience ◽  
2000 ◽  
Vol 35 (4) ◽  
pp. 745-748 ◽  
Author(s):  
Karen E. Hokanson ◽  
Margaret R. Pooler
Keyword(s):  
Callus Formation ◽  
Adventitious Shoot ◽  
Shoot Formation ◽  
Dichlorophenoxyacetic Acid ◽  
Naphthaleneacetic Acid ◽  
Adventitious Shoot Regeneration ◽  
Potential System ◽  
Regeneration In Vitro ◽  
2,4 Dichlorophenoxyacetic Acid

Callus formation and adventitious shoot regeneration in vitro from mature stored seed were evaluated in eight ornamental cherry (Prunus) taxa: P. campanulata Maxim., P. maackii Rupr., P. sargentii Rehd., P. serrula Franch., P. serrulata Lindl., P. subhirtella Miq., P. virginiana L., and P. yedoensis Matsum. Several portions of the embryo (cotyledons and hypocotyl sections) and nine combinations of growth regulators (BA, 2,4-D, IBA, NAA, and TDZ) were compared. Effects of embryo portions and growth regulator treatments were generally small within taxa, but shoot formation differed among taxa. About 20% to 50% of the embryos from P. virginiana and P. serrula and ≈5% to 30% of those from P. maackii produced shoots. The other taxa generally did not produce shoots. Regeneration from mature stored seed in the responsive taxa represents a potential system for genetic transformation. Chemical names used: 6-benzyladenine (BA); 2,4-dichlorophenoxyacetic acid (2,4-D); indole-3-butyric acid (IBA); α-naphthaleneacetic acid (NAA); thidiazuron (TDZ).

Induction de l'organogénèse sur des cals de Vicia faba minor provenant d'apex

1981 ◽  
Vol 59 (2) ◽  
pp. 203-207 ◽  
Author(s):  
Rose Galzy ◽  
Mahmoud Hamoui
Keyword(s):  
Vicia Faba ◽  
Shoot Formation ◽  
Cultivated Plants ◽  
Dichlorophenoxyacetic Acid ◽  
Naphthaleneacetic Acid ◽  
Growth Substances ◽  
Effective Combination ◽  
Vicia Faba Minor ◽  
2,4 Dichlorophenoxyacetic Acid

In vitro cultivated plants of Vicia faba minor were propagated by microcutting and used as the initial material for these experiments. Shoot tips were first cultivated on callus-inducing media. The transfer of calluses to media containing lower levels of growth substances resulted, in some instances, in the neoformation of roots and shoots. Rooted plantlets were then obtained from these shoots.Auxins (α-naphthaleneacetic acid (NAA) and 2,4-dichlorophenoxyacetic acid (2,4-D)) and cytokinins (6-benzylaminopurine (6-BAP) and 6-furfurylaminopurine (kinetin)) clearly influenced the rate of callus growth and the capacity for organogenesis. They were mixed together to give four callus-inducing media, each containing an auxin (1 mg/L) and a cytokinin (5 mg/L). The media tested for organogenesis contained in each case the same growth substances as the initiation medium but the concentration of auxin and cytokinin was reduced and their ratio modified. Shoot differentiation occurred only when the auxin used was NAA. The most effective combination for shoot formation was the following: 0.1 mg/L NAA and 0.5 mg/L 6-BAP.

scholarly journals Haploid Induction via In Vitro Gynogenesis in Persian Shallot (Allium hirtifolium)

2019 ◽  
Vol 27 (2) ◽  
pp. 91-98
Author(s):  
Jaber Panahandeh ◽  
Nasrin Farhadi
Keyword(s):  
Somatic Tissue ◽  
Dichlorophenoxyacetic Acid ◽  
Naphthaleneacetic Acid ◽  
Haploid Induction ◽  
Breeding Programs ◽  
Genetic Studies ◽  
The Media ◽  
2,4 Dichlorophenoxyacetic Acid ◽  
Allium Hirtifolium

AbstractHaploid induction using in vitro cultures of unpollinated flowers has been recognized as an important tool to produce homozygous plants for genetic studies and breeding programs. In this study the potential of gynogenic haploid induction in four ecotypes of Allium hirtifolium under different combinations of benzylaminopurine (BAP) with 2,4-dichlorophenoxyacetic acid (2,4-D), or α-naphthaleneacetic acid (NAA) was investigated. Unpollinated flower buds were excised from an umbel 5 to 3 days before anthesis, and cultured onto B5 medium containing 7.5% sucrose and 2 mg·dm−3 BAP with auxin. The experiments revealed that NAA increased the percentage of gynogenesis induction and number of gynogenic embryos per flower in all ecotypes. Somatic organogenesis from basal callus or other floral parts was most effective on the media containing 2,4-D. Plants obtained by gynogenesis were haploid in 70–77% and plants from somatic tissue were mostly diploid.

scholarly journals In Vitro Propagation of Miscanthus sinensis

HortScience ◽  
1990 ◽  
Vol 25 (10) ◽  
pp. 1291-1293 ◽  
Author(s):  
N.J. Gawel ◽  
C.D. Robacker ◽  
W.L. Corley
Keyword(s):  
In Vitro Propagation ◽  
Miscanthus Sinensis ◽  
Dichlorophenoxyacetic Acid ◽  
Ms Medium ◽  
Callus Initiation ◽  
Half Strength ◽  
Modified Ms Medium ◽  
D 20 ◽  
2,4 Dichlorophenoxyacetic Acid

Immature inflorescences of Miscanthus sinensis Andress. `Gracillimus', `Variegatus', and `Zebrinus' were cultured on modified MS medium with 9.0 μm 2,4-D, 20 g sucrose/liter, 2.0 g Gelrite/liter, and 0.75 g MgCl2/liter. Organogenesis was observed 8 to 12 weeks after callus initiation. Shoots were rooted on half-strength MS medium without growth regulators. After rooting, tillers were initiated. When transferred to soil, plants matured to flowering quickly and retained their variegation patterns. Propagation through in vitro tillering is suggested. Chemical name used: 2,4-dichlorophenoxyacetic acid (2,4-D).

scholarly journals In vitro germination and bulblet and shoot propagation for wild edible Eremurus spectabilis M.Bieb.

2020 ◽  
Vol 48 (2) ◽  
pp. 814-825
Author(s):  
Burcu TUNCER
Keyword(s):  
In Vitro Propagation ◽  
Shoot Formation ◽  
Dichlorophenoxyacetic Acid ◽  
In Vitro Germination ◽  
Cut Flower ◽  
Nutrient Media ◽  
The Third ◽  
2,4 Dichlorophenoxyacetic Acid ◽  
Shoot Propagation

Eremurus spectabilis M.Bieb is consumed as a vegetable because of its nutritious characteristics. The plants are also used for medicinal purposes, in the cut flower industry as an ornamental geophytes, and in industry as a natural adhesive. The aim of the present study was to improve the in vitro propagation protocol (in vitro germination and bulblet and shoot formation) of E. spectabilis. For this purpose, E. spectabilis seeds were in vitro germinated in four different nutrient media: Murashige and Skoog (MS), Gamborg (B5), White (WH), and Shenk and Hildebrandt (SH). To stimulate bulblet and/or shoot regeneration, hypocotyls of 35-40-day-old in vitro-germinated plantlets were cut into 0.5-1.0 cm pieces, and the resultant explants were cultured in MS media containing 2,4-dichlorophenoxyacetic acid (2,4-D) (0.5, 1.0, 2.0, and 4.0 mg L-1) + Kinetin (0.5 mg L-1), Thidiazuron (TDZ) (0.5, 1.0, 2.0, and 4.0 mg L-1) + 1-Naphthylacetic acid (NAA) (0, 0.1, 0.5, and 1.0 mg L-1), and 6-Benzylaminopurine (BAP) (0.5, 1.0, 2.0, and 4.0 mg L-1) + 2,4-dichlorophenoxyacetic acid (2,4-D) (0, 0.1, 0.5, and 1.0 mg L-1). The best outcomes for germination ratio (57.5%) were obtained from the B5 medium. In the third set of in vitro propagation experiments, 100% bulblet formation was achieved in TDZ (0.5 mg L-1) and NAA (0.5 and 0.1 mg L-1) combinations of MS media, and this was followed by 0.5 mg L-1 BAP-containing medium (81.3%). Shoot formation ratios with the same media combinations varied from 60-70%, and the number of shoots per explant varied from 1.4-2.4 shoots. Further in vitro propagation research is planned with larger bulb sizes to develop a protocol for rooting bulblets and/or shoots.

scholarly journals Improving In Vitro Plant Regeneration from Leaf and Petiole Explants of `Marion' Blackberry

HortScience ◽  
2004 ◽  
Vol 39 (2) ◽  
pp. 316-320 ◽  
Author(s):  
Rengong Meng ◽  
Tony H.H. Chen ◽  
Chad E. Finn ◽  
Yonghai Li
Keyword(s):  
Leaf Explants ◽  
Petri Dish ◽  
Shoot Formation ◽  
Photon Flux ◽  
Dichlorophenoxyacetic Acid ◽  
Naphthaleneacetic Acid ◽  
Petiole Explants ◽  
2,4 Dichlorophenoxyacetic Acid ◽  
In Vitro Shoot Regeneration

Experiments focusing on plant growth regulators' concentrations and combinations, mineral salt formulations, and TDZ pretreatment formations were conducted to optimize in vitro shoot regeneration from leaf and petiole explants of `Marion' blackberry. Optimum shoot formation was obtained when stock plants were incubated in TDZ pretreatment medium for 3 weeks before culturing leaf explants on regeneration medium (Woody Plant Medium with 5 μm BA and 0.5 μm IBA) in darkness for 1 week before transfer to light photoperiod (16-hour photoperiod at photosynthetic photon flux of ≈50 μmol·m-2·s-1) at 23 °C ± 2 °C for 4 weeks. Under these conditions, ≈70% of leaf explants formed ≈40 shoots per petri dish that could be harvested and rooted to form plantlets. Chemical names used: N6-benzyladenine (BA); 2,4-dichlorophenoxyacetic acid (2,4-D); gibberellic acid (GA3); indole-3-acetic acid (IAA); indole-3-butyric acid (IBA); α-naphthaleneacetic acid (NAA); N-phenyl-N'-1,2,3-thidiazol-5-ylurea [thidiazuron (TDZ)].

scholarly journals Optimization of Callogenesis/Caulogenesis Induction Protocol in Saffron Plant (Crocus sativus L.) Using Response Surface Methodology

2021 ◽  
Vol 12 (4) ◽  
pp. 4731-4746
Keyword(s):  
Callus Formation ◽  
In Vitro Regeneration ◽  
Shoot Formation ◽  
Crocus Sativus ◽  
Dichlorophenoxyacetic Acid ◽  
Naphthaleneacetic Acid ◽  
Regeneration System ◽  
Indole Butyric Acid ◽  
2,4 Dichlorophenoxyacetic Acid

The Crocus sativus, an endangered medicinal and aromatic plant in Morocco, has a low propagation rate in natural conditions and, therefore, an efficient method for in vitro propagation is required. This study investigated the effects of various hormones on the induction of callogenesis and callogenesis in C. sativus corms using the Box-Behnken experimental design. The best shoot formation was obtained with Murashige and Skoog fortified with 3 mg/L 6-Benzylaminopurine. On the other hand, callus formation was obtained with 3 mg/L 1-Naphthaleneacetic Acid or 3 mg/L 2,4-Dichlorophenoxyacetic Acid. However, a combination of 3 mg/L 6-Benzylaminopurine, 1.056 mg/L Indole Butyric Acid, and 3 mg/L 2,4-Dichlorophenoxyacetic Acid allows 50% caulogenesis and 60% callogenesis. The in vitro regeneration system could be utilized for both conservation and largescale multiplication of Crocus sativus corms.

Evaluation of birdsfoot-trefoil (Lotus corniculatus) regenerated plants following in vitro selection for herbicide tolerance

1987 ◽  
Vol 65 (6) ◽  
pp. 1275-1280 ◽  
Author(s):  
Nancy L. MacLean ◽  
W. F. Grant
Keyword(s):  
Chromosome Number ◽  
In Vitro Selection ◽  
Selection Process ◽  
Herbicide Tolerance ◽  
Lotus Corniculatus ◽  
Dichlorophenoxyacetic Acid ◽  
Regenerated Plants ◽  
Selection For ◽  
2,4 Dichlorophenoxyacetic Acid

Suspension cultures of Lotus corniculatus L., cv. Leo, were treated with the herbicides 2,4-D (2,4-dichlorophenoxyacetic acid) (10–20 ppm) or chlorsulfuron (2-chloro-N-[[(4-methoxy-6-methyl-1,3,5-triazin-2-yl)amino]carbonyl]-benzenesulfonamide) (0.5–1.0 ppb) to select for either herbicide resistance or tolerance. Effects of the selection process on regeneration, pollen, chromosome number, and herbicide sensitivity of regenerates were evaluated. Plantlets regenerated from selective 2,4-D cultures exhibited low in vitro rooting percentage, a high percentage of shrivelled pollen, and variation in chromosome number. The effect of 2,4-D was confounded, in some cases, with the effect of duration of culture period. A slight degree of tolerance was observed in one of the selections to chlorsulfuron at 10 to 20 g ha−1. No sign of tolerance to 2,4-D was observed at 1.0 kg ha−1.

In Vitro Shoot Regeneration from Callus Derived from Marubakaido Apple Rootstock under Different Aluminium Concentrations

HortScience ◽  
1998 ◽  
Vol 33 (3) ◽  
pp. 460e-460 ◽  
Author(s):  
Marisa F. de Oliveira ◽  
Gerson R. de L. Fortes ◽  
João B. da Silva
Keyword(s):  
Shoot Regeneration ◽  
Dichlorophenoxyacetic Acid ◽  
Apple Rootstock ◽  
Under Five ◽  
Significant Difference ◽  
Previously Treated ◽  
2,4 Dichlorophenoxyacetic Acid ◽  
In Vitro Shoot Regeneration

The aim of this work was to evaluate the organogenesis of Marubakaido apple rootstock under different aluminium concentratons. The explants were calli derived from apple internodes treated with either 2,4-dichlorophenoxyacetic acid or pichloram at 0.5 and 1.0 μM and under five different aluminium concentrations (0, 5, 10, 15, 20 mg/L). These calli were then treated with aluminium at 0, 5, 10, 15, and 20 mg/L. It was observed shoot regeneration only for those calli previously treated with pichloram. There were no significant difference among the aluminium concentrations.

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