Molecular cloning of cucumber phosphoenolpyruvate carboxykinase and developmental regulation of gene expression

1994 ◽  
Vol 26 (1) ◽  
pp. 423-434 ◽  
Author(s):  
Dae-Jae Kim ◽  
Steven M. Smith
Keyword(s):  
Gene Expression ◽  
Molecular Cloning ◽  
Phosphoenolpyruvate Carboxykinase ◽  
Developmental Regulation ◽  
Regulation Of Gene Expression

scholarly journals Differential regulation of the rat phosphoenolpyruvate carboxykinase gene expression in several tissues of transgenic mice.

1992 ◽  
Vol 12 (3) ◽  
pp. 1396-1403 ◽  
Author(s):  
C L Eisenberger ◽  
H Nechushtan ◽  
H Cohen ◽  
M Shani ◽  
L Reshef
Keyword(s):  
Gene Expression ◽  
Adipose Tissue ◽  
Transgenic Mice ◽  
Phosphoenolpyruvate Carboxykinase ◽  
Developmental Regulation ◽  
Regulation Of Gene Expression ◽  
Regulatory Sequences ◽  
Specific Expression ◽  
Endogenous Gene ◽  
Flanking Region

The selective expression of a unique copy gene in several mammalian tissues has been approached by studying the regulatory sequences needed to control expression of the rat phosphoenolpyruvate carboxykinase (PEPCK) gene in transgenic mice. A transgene containing the entire PEPCK gene, including 2.2 kb of the 5'-flanking region and 0.5 kb of the 3'-flanking region, exhibits tissue-specific expression in the liver, kidney, and adipose tissue, as well as the hormonal and developmental regulation inherent to endogenous gene expression. Deletions of the 5'-flanking region of the gene have shown the need for sequences downstream of position -540 of the PEPCK gene for expression in the liver and sequences downstream of position -362 for expression in the kidney. Additional sequences upstream of position -540 (up to -2200) are required for expression in adipose tissue. In addition, the region containing the glucocorticoid-responsive elements of the gene used by the kidney was identified. This same sequence was found to be needed specifically for developmental regulation of gene expression in the kidney and, together with upstream sequences, in the intestine. The apparently distinct sequence requirements in the various tissues indicate that the tissues use different mechanisms for expression of the same gene.

Differential regulation of the rat phosphoenolpyruvate carboxykinase gene expression in several tissues of transgenic mice

1992 ◽  
Vol 12 (3) ◽  
pp. 1396-1403
Author(s):  
C L Eisenberger ◽  
H Nechushtan ◽  
H Cohen ◽  
M Shani ◽  
L Reshef
Keyword(s):  
Gene Expression ◽  
Adipose Tissue ◽  
Transgenic Mice ◽  
Phosphoenolpyruvate Carboxykinase ◽  
Developmental Regulation ◽  
Regulation Of Gene Expression ◽  
Regulatory Sequences ◽  
Specific Expression ◽  
Endogenous Gene ◽  
Flanking Region

The selective expression of a unique copy gene in several mammalian tissues has been approached by studying the regulatory sequences needed to control expression of the rat phosphoenolpyruvate carboxykinase (PEPCK) gene in transgenic mice. A transgene containing the entire PEPCK gene, including 2.2 kb of the 5'-flanking region and 0.5 kb of the 3'-flanking region, exhibits tissue-specific expression in the liver, kidney, and adipose tissue, as well as the hormonal and developmental regulation inherent to endogenous gene expression. Deletions of the 5'-flanking region of the gene have shown the need for sequences downstream of position -540 of the PEPCK gene for expression in the liver and sequences downstream of position -362 for expression in the kidney. Additional sequences upstream of position -540 (up to -2200) are required for expression in adipose tissue. In addition, the region containing the glucocorticoid-responsive elements of the gene used by the kidney was identified. This same sequence was found to be needed specifically for developmental regulation of gene expression in the kidney and, together with upstream sequences, in the intestine. The apparently distinct sequence requirements in the various tissues indicate that the tissues use different mechanisms for expression of the same gene.

Molecular cloning of schistosome genes

Parasitology ◽  
1986 ◽  
Vol 92 (S1) ◽  
pp. S73-S81 ◽  
Author(s):  
D. W. Taylor ◽  
J. S. Cordingley ◽  
D. W. Dunne ◽  
K. S. Johnson ◽  
W. J. Haddow ◽  
...  
Keyword(s):  
Gene Expression ◽  
Life Cycle ◽  
Molecular Cloning ◽  
Sexual Maturity ◽  
Egg Production ◽  
Developmental Regulation ◽  
Regulation Of Gene Expression ◽  
Surface Antigens ◽  
Vector Systems ◽  
Epidemiological Surveys

As part of an integrated programme investigating human schistosomiasis, work which involves epidemiological surveys and detailed immunological studies as well as biochemical investigations, we have, over the last three years, been cloning schistosome genes in a variety of plasmid and lambda vector systems. In this lecture we present a review of some selected aspects of work primarily aimed at production of experimental vaccines against the disease but which, on a broader front, is also concerned with developmental regulation of gene expression around the parasite's life-cycle. Specifically, we are interested in cloning three groups of genes. First, those encoding surface antigens; second, those associated with sexual maturity and egg production; and third, antigens which may provide a basis for a specific immunodiagnostic test.

Developmental Regulation of Gene Expression during Barley Endosperm Formation

1995 ◽  
Vol 145 (5-6) ◽  
pp. 587-591 ◽  
Author(s):  
O.-A. Olsen ◽  
R. Brown ◽  
R. Kalla ◽  
L.A. Kleczkowski ◽  
B. Lemmon ◽  
...  
Keyword(s):  
Gene Expression ◽  
Developmental Regulation ◽  
Regulation Of Gene Expression ◽  
Barley Endosperm ◽  
Endosperm Formation
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