Expression of the potato leafroll luteovirus coat protein gene in transgenic potato plants inhibits viral infection

1991 ◽  
Vol 17 (3) ◽  
pp. 431-439 ◽  
Author(s):  
Frank van der Wilk ◽  
Dinie Posthumus-Lutke Willink ◽  
Marianne J. Huisman ◽  
Harm Huttinga ◽  
Rob Goldbach
Keyword(s):  
Coat Protein ◽  
Viral Infection ◽  
Coat Protein Gene ◽  
Protein Gene ◽  
Transgenic Potato ◽  
Potato Plants ◽  
Transgenic Potato Plants ◽  
Potato Leafroll Luteovirus

Transgenic potato plants expressing the potato virus X (PVX) coat protein gene developed resistance to the viral infection

2002 ◽  
Vol 30 (2) ◽  
pp. 177-185 ◽  
Author(s):  
V. Doreste ◽  
P. L. Ramos ◽  
G. A. Enríquez ◽  
R. Rodríguez ◽  
R. Peral ◽  
...  
Keyword(s):  
Coat Protein ◽  
Viral Infection ◽  
Potato Virus ◽  
Coat Protein Gene ◽  
Protein Gene ◽  
Potato Virus X ◽  
Transgenic Potato ◽  
Potato Plants ◽  
Transgenic Potato Plants

scholarly journals PVYNTN-CP coat protein gene mediated virus resistance of transgenic potato plants

2009 ◽  
Vol 7 (4) ◽  
pp. 41-50 ◽  
Author(s):  
Zenon Stasevski ◽  
Olga N Ilinskaya
Keyword(s):  
Coat Protein ◽  
Coat Protein Gene ◽  
Virus Resistance ◽  
Protein Gene ◽  
Solanum Tuberosum L ◽  
Transgenic Potato ◽  
Potato Plants ◽  
Transgenic Potato Plants ◽  
Transgenic Potatoes ◽  
Virus Strains

PVY<sup style="line-height:1.6em">NTN</sup>-CP <span style="line-height:1.6em">coat protein gene from a necrotic strain of potato virus </span>Y (pvy<sup style="line-height:1.6em">ntn</sup>) <span style="line-height:1.6em">has been transferred into two potato </span>Solanum tuberosum L. <span style="line-height:1.6em">cultivars </span>Mindenes <span style="line-height:1.6em">and </span>Somogyi kifli via Agrobacterium tumefaciens <span style="line-height:1.6em">transformation. Expression of integrated PVY</span><sup style="line-height:1.6em">NTN</sup><span style="line-height:1.6em">-CP gene were confirmed for 33 (89 %) of 37 and 3 (75 %) of 4 kanamycin-resistant regenerants of potato cultivars Mindenes and Somogyi kifli respectively. The level of virus resistance against two virus strains </span>(PVY°, PVY<sup style="line-height:1.6em">NTN</sup>) <span style="line-height:1.6em">of independent lines of transgenic potatoes varied between extreme resistance to susceptibility. The three independent lines of transgenic potatoes proved to be extreme resistant against both PVY strains.</span>

Expression of PVX coat protein gene under the control of extensin-gene promoter confers virus resistance on transgenic potato plants

1992 ◽  
Vol 11 (1) ◽  
Author(s):  
Attila Feh�r ◽  
KonstantinG. Skryabin ◽  
Ervin Bal�zs ◽  
Johanna Preiszner ◽  
OlgaA. Shulga ◽  
...  
Keyword(s):  
Coat Protein ◽  
Coat Protein Gene ◽  
Virus Resistance ◽  
Protein Gene ◽  
Gene Promoter ◽  
Transgenic Potato ◽  
Potato Plants ◽  
Transgenic Potato Plants

scholarly journals Assembly of virus-like particles in insect cells infected with a baculovirus containing a modified coat protein gene of potato leafroll luteovirus

1996 ◽  
Vol 77 (7) ◽  
pp. 1349-1358 ◽  
Author(s):  
J. W. Lamb ◽  
G. H. Duncan ◽  
B. Reavy ◽  
F. E. Gildow ◽  
M. A. Mayo ◽  
...  
Keyword(s):  
Coat Protein ◽  
Coat Protein Gene ◽  
Insect Cells ◽  
Protein Gene ◽  
Virus Like Particles ◽  
Potato Leafroll Luteovirus

scholarly journals Expression of the Potato Leafroll Virus ORF0 Induces Viral-Disease-like Symptoms in Transgenic Potato Plants

1997 ◽  
Vol 10 (2) ◽  
pp. 153-159 ◽  
Author(s):  
Frank van der Wilk ◽  
Petra Houterman ◽  
Jos Molthoff ◽  
Fabienne Hans ◽  
Ben Dekker ◽  
...  
Keyword(s):  
Dna Sequences ◽  
Transgenic Potato ◽  
Viral Disease ◽  
Host Recognition ◽  
Reading Frame ◽  
Potato Plants ◽  
Transgenic Potato Plants ◽  
Potato Leafroll Luteovirus ◽  
Leafroll Virus

The role of the open reading frame 0 (ORF0) of luteoviruses in the viral infection cycle has not been resolved, although the translation product (p28) of this ORF has been suggested to play a role in host recognition. To investigate the function of the potato leafroll luteovirus (PLRV) p28 protein, transgenic potato plants were produced containing the ORF0. In the lines in which the ORF0 transcripts could be detected by Northern (RNA) analysis, the plants displayed an altered phenotype resembling virus-infected plants. A positive correlation was observed between levels of accumulation of the transgenic transcripts and severity of the phenotypic aberrations observed. In contrast, potato plants transformed with a modified, untranslatable ORF0 sequence were phenotypically indistinguishable from wild-type control plants. These results suggest that the p28 protein is involved in viral symptom expression. Southern blot analysis showed that the transgenic plants that accumulated low levels of ORF0 transcripts detectable only by reverse transcription-polymerase chain reaction, contained methylated ORF0 DNA sequences, indicating down-regulation of the transgene provoked by the putatively unfavorable effects p28 causes in the plant cell.

scholarly journals In SituLocalization of the Putative Movement Protein (pr17) from Potato Leafroll Luteovirus (PLRV) in Infected and Transgenic Potato Plants

Virology ◽  
1997 ◽  
Vol 235 (2) ◽  
pp. 311-322 ◽  
Author(s):  
J. Schmitz ◽  
C. Stussi-Garaud ◽  
E. Tacke ◽  
D. Prüfer ◽  
W. Rohde ◽  
...  
Keyword(s):  
Movement Protein ◽  
Transgenic Potato ◽  
Potato Plants ◽  
Transgenic Potato Plants ◽  
Putative Movement Protein ◽  
Potato Leafroll Luteovirus
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