scholarly journals PVYNTN-CP coat protein gene mediated virus resistance of transgenic potato plants

2009 ◽  
Vol 7 (4) ◽  
pp. 41-50 ◽  
Author(s):  
Zenon Stasevski ◽  
Olga N Ilinskaya
Keyword(s):  
Coat Protein ◽  
Coat Protein Gene ◽  
Virus Resistance ◽  
Protein Gene ◽  
Solanum Tuberosum L ◽  
Transgenic Potato ◽  
Potato Plants ◽  
Transgenic Potato Plants ◽  
Transgenic Potatoes ◽  
Virus Strains

PVY<sup style="line-height:1.6em">NTN</sup>-CP <span style="line-height:1.6em">coat protein gene from a necrotic strain of potato virus </span>Y (pvy<sup style="line-height:1.6em">ntn</sup>) <span style="line-height:1.6em">has been transferred into two potato </span>Solanum tuberosum L. <span style="line-height:1.6em">cultivars </span>Mindenes <span style="line-height:1.6em">and </span>Somogyi kifli via Agrobacterium tumefaciens <span style="line-height:1.6em">transformation. Expression of integrated PVY</span><sup style="line-height:1.6em">NTN</sup><span style="line-height:1.6em">-CP gene were confirmed for 33 (89 %) of 37 and 3 (75 %) of 4 kanamycin-resistant regenerants of potato cultivars Mindenes and Somogyi kifli respectively. The level of virus resistance against two virus strains </span>(PVY°, PVY<sup style="line-height:1.6em">NTN</sup>) <span style="line-height:1.6em">of independent lines of transgenic potatoes varied between extreme resistance to susceptibility. The three independent lines of transgenic potatoes proved to be extreme resistant against both PVY strains.</span>

Expression of PVX coat protein gene under the control of extensin-gene promoter confers virus resistance on transgenic potato plants

1992 ◽  
Vol 11 (1) ◽  
Author(s):  
Attila Feh�r ◽  
KonstantinG. Skryabin ◽  
Ervin Bal�zs ◽  
Johanna Preiszner ◽  
OlgaA. Shulga ◽  
...  
Keyword(s):  
Coat Protein ◽  
Coat Protein Gene ◽  
Virus Resistance ◽  
Protein Gene ◽  
Gene Promoter ◽  
Transgenic Potato ◽  
Potato Plants ◽  
Transgenic Potato Plants

Expression of the potato leafroll luteovirus coat protein gene in transgenic potato plants inhibits viral infection

1991 ◽  
Vol 17 (3) ◽  
pp. 431-439 ◽  
Author(s):  
Frank van der Wilk ◽  
Dinie Posthumus-Lutke Willink ◽  
Marianne J. Huisman ◽  
Harm Huttinga ◽  
Rob Goldbach
Keyword(s):  
Coat Protein ◽  
Viral Infection ◽  
Coat Protein Gene ◽  
Protein Gene ◽  
Transgenic Potato ◽  
Potato Plants ◽  
Transgenic Potato Plants ◽  
Potato Leafroll Luteovirus

Transgenic potato plants expressing the potato virus X (PVX) coat protein gene developed resistance to the viral infection

2002 ◽  
Vol 30 (2) ◽  
pp. 177-185 ◽  
Author(s):  
V. Doreste ◽  
P. L. Ramos ◽  
G. A. Enríquez ◽  
R. Rodríguez ◽  
R. Peral ◽  
...  
Keyword(s):  
Coat Protein ◽  
Viral Infection ◽  
Potato Virus ◽  
Coat Protein Gene ◽  
Protein Gene ◽  
Potato Virus X ◽  
Transgenic Potato ◽  
Potato Plants ◽  
Transgenic Potato Plants

scholarly journals Design of expression cassettes using the Cry1Ba1 gene for potato (Solanum tuberosum L.) varieties

2019 ◽  
Vol 37 (2) ◽  
pp. 103-111
Author(s):  
Natalyth Erira ◽  
Alejandro Chaparro-Giraldo ◽  
Silvio López-Pazos
Keyword(s):  
Solanum Tuberosum L ◽  
Potato Crop ◽  
Insect Pest ◽  
Transgenic Potato ◽  
Economic Losses ◽  
Potato Plants ◽  
Commercial Use ◽  
Tecia Solanivora ◽  
Transgenic Potato Plants ◽  
Polyadenylation Signals

The most serious insect pest problem in the potato crop in Colombia is the lepidopteran Tecia solanivora that causessignificant economic losses. In this research, we designedexpression cassettes based on the cry1Ba1 gene of Bacillusthuringiensis that could confer resistance to T. solanivora via the variety Pastusa Suprema. We selected the elements of thedesigned expression cassettes through an analysis of scientificliterature and patent databases; the considered factors were theproteolytic activation of the Cry1Ba1 protoxin, modification ofcodonic use, polyadenylation signals, and cryptic splicing sites. We used a tissue-specific patatine promoter to reduce potentialbiosafety risks, because it is expressed only in the tuber. Thefreedom to operate analysis suggests that the commercial use of the designed expression cassettes in transgenic potato plants does not affect the rights of third parties in Colombia.

Application of genetic engineering in potato breeding

2010 ◽  
Vol 58 (4) ◽  
pp. 427-441 ◽  
Author(s):  
A. Gorji ◽  
Z. Polgar
Keyword(s):  
Potato Breeding ◽  
Rapid Change ◽  
Transgenic Potato ◽  
Potato Plants ◽  
Wide Range ◽  
Breeding Programmes ◽  
Transgenic Potato Plants ◽  
Transgenic Potatoes ◽  
Plant Pathogen Interactions ◽  
The Impact

Potato breeding programmes worldwide are undergoing a period of rapid change. In order to be successful, breeders must adapt and incorporate the newest up-to-date techniques as they become available. Recent advances in biotechnology make it possible to develop and cultivate more and more sophisticated transgenic crops with multiple modified traits. Gene transfer methods can be used for a wide range of fundamental studies, contributing to a better understanding of the mechanisms of plant/pathogen interactions and the metabolic pathways in plants. Transgenic potato plants are being generated worldwide to investigate the impact of transgene expression on parameters as complex as yield. Historically, potato was one of the first successfully transformed crop plants. Nowadays, transgenic potatoes have been introduced into the food chain of people and animals in several countries. Some of the genetic modifications give potato plants increased resistance to biotic and abiotic environmental factors, while others lead to improved nutritional value, or cause the plants to produce proteins of the immune system of humans or animals or substances that may be used as vaccines in humans or veterinary medicine. The trend today is towards the generation of crops with output traits, e.g. modified starch or carotenoids, or the production of pharmaceuticals in tubers, whereas the early targets were input traits, e.g. herbicide resistance, pest or virus resistance. This review provides a summary of examples illustrating the versatility and applicability of transgenic biology in potato improvement.

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