l-Malic acid production from fumaric acid by a laboratory Saccharomyces cerevisiae strain SHY2

1996 ◽  
Vol 18 (12) ◽  
pp. 1441-1446 ◽  
Author(s):  
Xiaohai Wang ◽  
C. S. Gong ◽  
George T. Tsao
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Fumaric Acid ◽  
Malic Acid ◽  
Acid Production

Characteristics of the high malic acid production mechanism in Saccharomyces cerevisiae sake yeast strain No. 28

2012 ◽  
Vol 114 (3) ◽  
pp. 281-285 ◽  
Author(s):  
Shunichi Nakayama ◽  
Ken Tabata ◽  
Takahiro Oba ◽  
Kenichi Kusumoto ◽  
Shinji Mitsuiki ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Malic Acid ◽  
Yeast Strain ◽  
Acid Production ◽  
Production Mechanism

L-malic acid production using immobilized saccharomyces cerevisiae

1991 ◽  
Vol 30 (2) ◽  
pp. 217-224 ◽  
Author(s):  
Z. M. B. Figueiredo ◽  
L. B. Carvalho
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Malic Acid ◽  
Acid Production

L‐malic acid production from xylose by engineered Saccharomyces cerevisiae

2021 ◽  
pp. 2000431
Author(s):  
Nam Kyu Kang ◽  
Jae Won Lee ◽  
Donald R. Ort ◽  
Yong‐Su Jin
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Malic Acid ◽  
Acid Production ◽  
Engineered Saccharomyces Cerevisiae

scholarly journals Key Process Conditions for Production of C4 Dicarboxylic Acids in Bioreactor Batch Cultures of an Engineered Saccharomyces cerevisiae Strain

2009 ◽  
Vol 76 (3) ◽  
pp. 744-750 ◽  
Author(s):  
Rintze M. Zelle ◽  
Erik de Hulster ◽  
Wendy Kloezen ◽  
Jack T. Pronk ◽  
Antonius J. A. van Maris
Keyword(s):  
Carbon Dioxide ◽  
Saccharomyces Cerevisiae ◽  
Process Parameters ◽  
Malic Acid ◽  
Acid Production ◽  
Shake Flask ◽  
Simultaneous Optimization ◽  
Process Conditions ◽  
Recent Effort ◽  
Shake Flasks

ABSTRACT A recent effort to improve malic acid production by Saccharomyces cerevisiae by means of metabolic engineering resulted in a strain that produced up to 59 g liter−1 of malate at a yield of 0.42 mol (mol glucose)−1 in calcium carbonate-buffered shake flask cultures. With shake flasks, process parameters that are important for scaling up this process cannot be controlled independently. In this study, growth and product formation by the engineered strain were studied in bioreactors in order to separately analyze the effects of pH, calcium, and carbon dioxide and oxygen availability. A near-neutral pH, which in shake flasks was achieved by adding CaCO3, was required for efficient C4 dicarboxylic acid production. Increased calcium concentrations, a side effect of CaCO3 dissolution, had a small positive effect on malate formation. Carbon dioxide enrichment of the sparging gas (up to 15% [vol/vol]) improved production of both malate and succinate. At higher concentrations, succinate titers further increased, reaching 0.29 mol (mol glucose)−1, whereas malate formation strongly decreased. Although fully aerobic conditions could be achieved, it was found that moderate oxygen limitation benefitted malate production. In conclusion, malic acid production with the engineered S. cerevisiae strain could be successfully transferred from shake flasks to 1-liter batch bioreactors by simultaneous optimization of four process parameters (pH and concentrations of CO2, calcium, and O2). Under optimized conditions, a malate yield of 0.48 ± 0.01 mol (mol glucose)−1 was obtained in bioreactors, a 19% increase over yields in shake flask experiments.

Fumaric acid production in Saccharomyces cerevisiae by simultaneous use of oxidative and reductive routes

2013 ◽  
Vol 148 ◽  
pp. 91-96 ◽  
Author(s):  
Guoqiang Xu ◽  
Xiulai Chen ◽  
Liming Liu ◽  
Linghuo Jiang
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Fumaric Acid ◽  
Acid Production ◽  
Simultaneous Use

Malic acid production and consumption by selected of Saccharomyces cerevisiae under anaerobic and aerobic conditions

1984 ◽  
Vol 19 (6) ◽  
pp. 427-429 ◽  
Author(s):  
Fabrizio Fatichenti ◽  
Giovanni Antonio Farris ◽  
Pietrino Deiana ◽  
Salvatore Ceccarelli
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Malic Acid ◽  
Acid Production ◽  
Aerobic Conditions ◽  
Production And Consumption

Malic acid production through the whole-cell hydration of fumaric acid with immobilised Rhizopus oryzae

2018 ◽  
Vol 137 ◽  
pp. 152-161 ◽  
Author(s):  
Andre Naude ◽  
Willie Nicol
Keyword(s):  
Fumaric Acid ◽  
Malic Acid ◽  
Acid Production ◽  
Rhizopus Oryzae ◽  
Whole Cell

scholarly journals Fumaric Acid Production in Saccharomyces cerevisiae by In Silico Aided Metabolic Engineering

PLoS ONE ◽  
2012 ◽  
Vol 7 (12) ◽  
pp. e52086 ◽  
Author(s):  
Guoqiang Xu ◽  
Wei Zou ◽  
Xiulai Chen ◽  
Nan Xu ◽  
Liming Liu ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Metabolic Engineering ◽  
Fumaric Acid ◽  
In Silico ◽  
Acid Production
Sign in / Sign up

Export Citation Format

Share Document