Release of [3H]acetylcholine from the isolated rat or guinea-pig trachea evoked by preganglionic nerve stimulation; a comparison with transmural stimulation

1991 ◽  
Vol 344 (4) ◽  
Author(s):  
I. Wessler ◽  
A. Klein ◽  
D. Pohan ◽  
J. Maclagana ◽  
K. Rack�
Keyword(s):  
Guinea Pig ◽  
Nerve Stimulation ◽  
Transmural Stimulation ◽  
Isolated Rat

Perfusion of rat’s liver with blood: transmitter overflows and gluconeogenesis

1970 ◽  
Vol 174 (1037) ◽  
pp. 503-515 ◽  
Keyword(s):  
Guinea Pig ◽  
Rat Liver ◽  
Nerve Stimulation ◽  
Perfused Liver ◽  
Methyl Transferase ◽  
Isolated Rat Liver ◽  
Lactate Utilization ◽  
Transmitter Uptake ◽  
Sympathetic Transmitter ◽  
Isolated Rat

A simplified apparatus for the perfusion of the isolated rat liver is described. The operative technique has been developed so as to avoid any interruption in the blood supply to the liver. By the generally accepted criteria of viability the liver remains almost normal for up to 4 h. Perfusion with oxygenated blood through both the hepatic artery and portal vein confers no special advantages over portal perfusion alone, as judged by lactate utilization. No overflows of sympathetic transmitter could be detected from the perfused liver of the rat after nerve stimulation unless catechol- O -methyl transferase inhibitors were present; even in the presence of phenoxybenzamine (to block transmitter uptake by nerve endings) overflows were small. Larger transmitter overflows were obtained from the perfused liver of guinea-pig. Nerve stimulation caused a transient increase in the rate of gluconeogenesis by the rat liver; the maximum response, an increase of 60 %, occurred after 5 min stimulation at a frequency of 10 Hz.

Effect of atropine and DMAE (a hemicholinium derivative) on contractile responses of the guinea pig ileum

1969 ◽  
Vol 47 (2) ◽  
pp. 185-192 ◽  
Author(s):  
Marvin M. Goldenberg
Keyword(s):  
Smooth Muscle ◽  
Guinea Pig ◽  
Nerve Stimulation ◽  
Direct Action ◽  
Guinea Pig Ileum ◽  
Structural Analogue ◽  
Transmural Stimulation ◽  
Sustained Contraction ◽  
Intrinsic Excitation ◽  
Muscle Receptors

A close structural analogue of hemicholinium, α,α′-bis(dimethylammoniumacetaldehyde diethylacetal)-p,p′-diacetylbiphenyl bromide (DMAE), was reported to antagonize responses to ganglionic stimulants more than responses to preganglionic nerve stimulation in the cat or dog and to possess activity both similar to and different from cocaine. The present study was concerned with the effect of DMAE and atropine on the response of the isolated guinea pig ileum to ganglionic stimulants and to transmural stimulation. Nicotine and DMPP, ganglionic stimulants, caused initial spike-like contraction, which was blocked by atropine, followed by a slow sustained contraction, which was atropine-resistant but was inhibited by DMAE. Cocaine and morphine, unlike DMAE, did not elicit a differential antagonism of the biphasic effects of the ganglionic stimulants. Contractions evoked by transmural stimulation were, however, augmented by DMAE but reduced by cocaine and atropine. DMAE could induce contractions of the ileum, by means of intrinsic excitation of the cholinergic neuronal elements and not by a direct action on cholinergic smooth muscle receptors. In conclusion, ganglionic stimulants have revealed in the guinea pig ileum an atropine-resistant noncholinergic neuronal pathway which was sensitive to DMAE blockade. Furthermore, actions of DMAE and cocaine on contractions caused by ganglionic stimulants and transmural stimulation were found to be nonparallel.

Adenosine 5′-triphosphate release evoked by electrical nerve stimulation from the guinea-pig gallbladder

1987 ◽  
Vol 134 (1) ◽  
pp. 77-82 ◽  
Author(s):  
Toku Takahashi ◽  
Masato Kusunoki ◽  
Yoshio Ishikawa ◽  
Masaru Kantoh ◽  
Takehira Yamamura ◽  
...  
Keyword(s):  
Guinea Pig ◽  
Nerve Stimulation ◽  
Electrical Nerve Stimulation

Inhibition by α- and β-adrenoceptors of the twitch response to transmural stimulation in the guinea-pig vas deferens

1976 ◽  
Vol 40 (1) ◽  
pp. 153-162 ◽  
Author(s):  
Per Hedqvist ◽  
Ulf S. Von Euler
Keyword(s):  
Guinea Pig ◽  
Vas Deferens ◽  
Transmural Stimulation ◽  
Twitch Response

Effects of nerve stimulation on the spontaneous action potentials recorded in the proximal renal pelvis of the guinea-pig

1995 ◽  
Vol 23 (5) ◽  
pp. 343-350 ◽  
Author(s):  
R. J. Lang ◽  
Y. Zhang ◽  
B. Exintaris ◽  
F. Vogalis
Keyword(s):  
Guinea Pig ◽  
Nerve Stimulation ◽  
Renal Pelvis ◽  
Action Potentials ◽  
Spontaneous Action ◽  
Spontaneous Action Potentials
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