The IgG2a antibody response to thyroglobulin is linked to the Igh locus in mouse

The primary antigen-specific antibody response of various strains of mice to TEPC-15/PnC immune complexes has been examined. We found that both BALB/c and C3H mice were good responders to the PnC antigen; however, C3H mice were low responders, whereas BALB/c mice were high responders to the TEPC-15/PnC complexes. Using congenic strains on the C3H and BALB/c background, we have shown that the response to the complexes is not restricted by gene products of the H-2 complex or by the Igh (allotype) locus. However, responsiveness may be controlled by genes linked to the Igh locus, since we have shown that strains that are Ighj, Ighd, and Ighf are low responders, whereas strains that are Igha, Ighb, and Ighe are high responders to the immune complex. Moreover, responsiveness correlates with the expression of the T15 Id as measured using the anti-T15 monoclonal antibody, AB1-2. Thus, strains such as BALB/c, BALB.B, BALB.K, and CB-20, which express high levels of T15 (AB1-2) Id in their PFC response to PnC are relatively high responders to TEPC-15/PnC complexes, whereas C3H, C3H.SW, and C3H-OH, which express low levels of the T15 (AB1-2) Id, are low responders to the complexes. Finally, we found that BALB/c mice are high responders to complexes formed with T15+ antibodies, whereas they are low responders to complexes formed using T15- antibodies. The results suggest that the antigen-specific response to these immune complexes is Id-restricted.

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Presence of Phosphorylcholine on a Filarial Nematode Protein Influences Immunoglobulin G Subclass Response to the Molecule by an Interleukin-10-Dependent Mechanism

Infection and Immunity ◽

10.1128/iai.68.9.5466-5468.2000 ◽

2000 ◽

Vol 68 (9) ◽

pp. 5466-5468 ◽

Cited By ~ 45

Author(s):

Katrina M. Houston ◽

Emma H. Wilson ◽

Linda Eyres ◽

Frank Brombacher ◽

Margaret M. Harnett ◽

...

Keyword(s):

Antibody Response ◽

Immunoglobulin G ◽

Subcutaneous Injection ◽

Interleukin 10 ◽

Igg Subclasses ◽

Filarial Nematode ◽

Igg2a Antibody ◽

Significant Production ◽

Immunoglobulin G Subclass ◽

Dependent Mechanism

ABSTRACT The filarial nematode product ES-62 contains phosphorylcholine (PC) covalently attached to N-linked glycans. ES-62 induced high levels of immunoglobulin G1 (IgG1) antibodies, but no IgG2a, to non-PC epitopes of the molecule following subcutaneous injection into BALB/c mice. Conversely, mice given ES-62 lacking PC demonstrated significant production of both IgG subclasses. Thus, PC appears to block production of IgG2a antibodies to other epitopes on the parasite molecule. A role for interleukin-10 (IL-10) in this effect was shown by the ability of IL-10−/− mice to make an IgG2a antibody response to non-PC epitopes of ES-62.

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Chronic Active Hepatitis Induced by Helicobacter hepaticus in the A/JCr Mouse Is Associated with a Th1 Cell-Mediated Immune Response

Infection and Immunity ◽

10.1128/iai.66.7.3142-3148.1998 ◽

1998 ◽

Vol 66 (7) ◽

pp. 3142-3148 ◽

Cited By ~ 64

Author(s):

M. T. Whary ◽

T. J. Morgan ◽

C. A. Dangler ◽

K. J. Gaudes ◽

N. S. Taylor ◽

...

Keyword(s):

Immune Response ◽

Antibody Response ◽

Chronic Active Hepatitis ◽

Outer Membrane Proteins ◽

Interleukin 4 ◽

Polymorphonuclear Cells ◽

Helicobacter Hepaticus ◽

Igg2a Antibody ◽

Ifn Γ ◽

Th1 Immune Response

ABSTRACT Helicobacter hepaticus infection in A/JCr mice results in chronic active hepatitis characterized by perivascular, periportal, and parenchymal infiltrates of mononuclear and polymorphonuclear cells. This study examined the development of hepatitis and the immune response of A/JCr mice to H. hepaticus infection. The humoral and cell-mediated T helper immune response was profiled by measuring the postinfection (p.i.) antibody response in serum, feces, and bile and by the production of cytokines and proliferative responses by splenic mononuclear cells to H. hepaticusantigens. Secretory immunoglobulin A (IgA) and systemic IgG2a antibody developed by 4 weeks p.i. and persisted through 12 months. Splenocytes from infected mice proliferated and produced more gamma interferon (IFN-γ) than interleukin-4 (IL-4) or IL-5 when cultured with H. hepaticus outer membrane proteins. The predominantly IgG2a antibody response in serum and the in vitro production of IFN-γ in excess of IL-4 or IL-5 are consistent with a Th1 immune response reported in humans and mice infected withHelicobacter pylori and Helicobacter felis, respectively. Mice infected with H. hepaticus developed progressively severe perivascular, periportal, and hepatic parenchymal lesions consisting of lymphohistiocytic and plasmacytic cellular infiltrates. In addition, transmural typhlitis was observed at 12 months p.i. The characterization of a cell-mediated Th1 immune response toH. hepaticus infection in the A/JCr mouse should prove valuable as a model for experimental regimens which manipulate the host response to Helicobacter.

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