Complexity of antisperm antibody response by human lymphocytes deposited to SCID mice

1997 ◽  
Vol 56 (1-3) ◽  
pp. 271
Author(s):  
M Kurpisz
Keyword(s):  
Antibody Response ◽  
Human Lymphocytes ◽  
Scid Mice ◽  
Antisperm Antibody

Complexity of antisperm antibody response by human lymphocytes deposited to SCID mice

1997 ◽  
Vol 56 ◽  
pp. 271
Author(s):  
Maciej Kurpisz ◽  
Dorota Fiszer ◽  
Grant Gallagher
Keyword(s):  
Antibody Response ◽  
Human Lymphocytes ◽  
Scid Mice ◽  
Antisperm Antibody

Complex nature of the human antisperm antibody response in SCID mice

Andrologia ◽  
2004 ◽  
Vol 36 (2) ◽  
pp. 69-77 ◽  
Author(s):  
M. Kurpisz ◽  
D. Fiszer ◽  
G. Gallagher ◽  
M. Ugorski ◽  
A. Domagala ◽  
...  
Keyword(s):  
Antibody Response ◽  
Scid Mice ◽  
Complex Nature ◽  
Antisperm Antibody

Generation of primary antigen-specific human cytotoxic T lymphocytes in human/mouse radiation chimera

Blood ◽  
1996 ◽  
Vol 88 (2) ◽  
pp. 721-730 ◽  
Author(s):  
H Segall ◽  
I Lubin ◽  
H Marcus ◽  
A Canaan ◽  
Y Reisner
Keyword(s):  
T Cells ◽  
T Cell ◽  
Human Lymphocytes ◽  
Scid Mice ◽  
Human Antibody ◽  
Adoptive Therapy ◽  
Activation Markers ◽  
Human T Cell ◽  
Human T Cells

Severe combined immunodeficient (SCID) mice are increasingly used as hosts for the adoptive transfer of human lymphocytes. Human antibody responses can be obtained in these xenogeneic chimeras, but information about the functionality of the human T cells in SCID mice is limited and controversial. Studies using human peripheral blood lymphocytes (PBL) injected intraperitoneally (IP) into SCID mice (hu-PBL-SCID mice) have shown that human T cells from these chimeras are anergic and have a defective signaling via the T-cell receptor. In addition, their antigenic repertoire is limited to xenoreactive clones. In the present study, we tested the functionality of human T cell in a recently described chimeric model. In this system, BALB/c mice are conditioned by irradiation and then transplanted with SCID bone marrow, followed by IP injection of human PBL. Our experiments demonstrated that human T cells, recovered from these hu-PBL-BALB mice within 1 month posttransplant, proliferated and expressed activation markers upon stimulation with anti-CD3 monoclonal antibody. A vigorous antiallogeneic human cytotoxic T-lymphocyte (CTL) response could be generated in these mice by immunizing them with irradiated allogeneic cells. Moreover, anti-human immunodeficiency virus type 1 (HIV-1) Net- specific human CTLs could be generated in vivo from naive lymphocytes by immunization of mouse-human chimeras with a recombinant vaccinia-nef virus. This model may be used to evaluate potential immunomodulatory drugs or cytokines, and could provide a relevant model for testing HIV vaccines, for production of antiviral T-cell clones for adoptive therapy, and for studying human T-cell responses in vivo.

scholarly journals Generation of biliary lesions after transfer of human lymphocytes into severe combined immunodeficient (SCID) mice.

1989 ◽  
Vol 170 (6) ◽  
pp. 1919-1930 ◽  
Author(s):  
S M Krams ◽  
K Dorshkind ◽  
M E Gershwin
Keyword(s):  
Bile Duct ◽  
Human Lymphocytes ◽  
Severe Combined Immunodeficiency ◽  
Serum Levels ◽  
Scid Mice ◽  
Lymphoid Cells ◽  
Biliary Cirrhosis ◽  
Human Mononuclear Cell ◽  
Mononuclear Infiltrate ◽  
Immunodeficiency Disease

Human PBL have been reported to reconstitute B and T cells as well as human serum Ig in mice with severe combined immunodeficiency disease (SCID). To confirm these observations and attempt the transfer of an autoimmune disease to the immunodeficient animals, groups of SCID mice received an injection of PBL from patients with primary biliary cirrhosis (PBC) or from normal volunteers. By 8 wk after the injection of 10-42 x 10(6) PBL into the mice, human lymphoid cells were detected in the spleen of approximately half of the animals and all had detectable serum levels of human IgG. Moreover, the sera of SCID mice that received cells from patients with PBC contained human antimitochondrial antibodies (AMA) to dihydrolipoamide acetyltransferase, the major mitochondrial autoantigen of PBC. Histologically, a human mononuclear cell infiltrate was present around the portal areas of the liver and inflammation, bile duct atypica, and necrosis of bile duct cells were observed. While the biliary lesions in the SCID recipients of PBC cells were more severe, a mononuclear infiltrate was clearly evident in mice that received cells from normal donors, suggesting the presence of a graft-vs.-host-like disease. While these data are the first to describe an animal model with both the humoral and cellular characteristics of PBC, they also raise an interesting question regarding the preferential localization of lymphoid cells to the biliary system.

Gamma-globulin modulates growth of EBV-derived B-cell tumors in scid mice reconstituted with human lymphocytes

1993 ◽  
Vol 55 (5) ◽  
pp. 824-829 ◽  
Author(s):  
Mohammad R. Abedi ◽  
Birger Christensson ◽  
Salahuddin Al-Masud ◽  
Lennart Hammarström ◽  
C. I. Smith
Keyword(s):  
B Cell ◽  
Gamma Globulin ◽  
Human Lymphocytes ◽  
Scid Mice

scholarly journals Human Interleukin-15 Improves Engraftment of Human T Cells in NOD-SCID Mice

2006 ◽  
Vol 13 (2) ◽  
pp. 227-234 ◽  
Author(s):  
Anyuan Sun ◽  
Haiming Wei ◽  
Rui Sun ◽  
Weihua Xiao ◽  
Yongguang Yang ◽  
...  
Keyword(s):  
T Cell ◽  
T Lymphocytes ◽  
Human Lymphocytes ◽  
Scid Mice ◽  
Optimal Dosage ◽  
Dependent Manner ◽  
Human T Lymphocytes ◽  
Mouse Chimeras ◽  
T Lymphocyte Proliferation ◽  
Interleukin 15

ABSTRACT Human nonobese diabetic-severe combined immune deficiency (NOD-SCID) mouse chimeras have been widely used as an in vivo model to assess human immune function. However, only a small fraction of transferred human T lymphocytes can be detected in human peripheral blood lymphocyte (huPBL)-NOD-SCID chimeras. To improve the reconstitution of human T lymphocytes in NOD-SCID mice, the use of recombinant human interleukin-15 (rhIL-15) as a stimulator of human lymphocytes was explored. Administration of rhIL-15 after transplantation of huPBLs into NOD-SCID mice increased reconstitution of human T lymphocytes in a dose-dependent manner, with an optimal dosage of 1 μg/mouse. The number of human T lymphocytes (HLA-ABC+ CD3+) in the lymphoid organs or tissue of rhIL-15-treated huPBL-NOD-SCID mice increased 11- to 80-fold, and phytohemagglutinin-induced T-lymphocyte proliferation and cytokine production were significantly enhanced. Additionally, although mature human cells have not been thought to enter the murine thymus, human T lymphocytes were detected in the huPBL-NOD-SCID thymus after rhIL-15 treatment. Thus, rhIL-15 can be used to optimize long-term peripheral T-cell engraftment in these human-mouse chimeras and may also be useful in clinical treatment of T-cell deficiencies.

scholarly journals Screening and functional analysis of differentially expressed genes in an animal model ofEBV-associated lymphomas

2019 ◽  
Author(s):  
Yang Zhang ◽  
Chengkun Wang ◽  
Liangzhuan Liu ◽  
Qiu Peng ◽  
Xiaoning Gan ◽  
...  
Keyword(s):  
Malignant Transformation ◽  
Differentially Expressed Genes ◽  
Molecular Mechanisms ◽  
Expression Profiles ◽  
Human Lymphocytes ◽  
Ring Finger ◽  
Tumor Formation ◽  
Scid Mice ◽  
Differentially Expressed ◽  
Normal Human

AbstractEpstein-Barr virus(EBV) is an important human oncogenic virus. This paper is to explore how EBV induce malignant transformation of human lymphocytes and the related mechanism of lymphomagenesis. We have constructedhu-PBL/SCID chimeric miceand established a model of EBV-associated human-derived lymphomas. By using Agilent human whole genome microarray and a series of bioinformatic analyses, a total of 202 differentially expressed genes were screened from the EBV-induced lymphomas inhu-PBL/SCID mice, including 44 up-regulated and 158 down-regulated genes. Calculation of the rank score (RS) values of these genes in the HIPPIE protein interaction networks showed that topoisomerase II alpha (TOP2A), ubiquitin like with PHD and ring finger domains 1 (UHRF1), histone cluster 2 H2B family member E (HIST2H2BE), phosphoglycerate dehydrogenase (PHGDH), vinculin (VCL), insulin-like growth factor 1 receptor (IGF1R), Fos proto-oncogene (FOS), snail family transcriptional repressor 1 (SNAI1), PDZ binding kinase (PBK), and ring finger protein 144B (RNF144B) were the top 10 key node genes of EBV-induced lymphoma. In which, PBK, an up-regulated genes with the highest number of GO annotations, was verified by cellular function experiments and clinical lymphoma samples.Author summaryEB virus is closely associated with human lymphoma and nasopharyngeal carcinoma. Since the susceptible hosts of EBV limit to human and cottontop tammarins, there are no appropriate animal models so far to study the EBV-associated oncogenesis. In our previous experiments, the EBV-associated lymphomas were induced inhu-PBL/SCID chimera(a new humanized mouse model). However, the cellular and molecular mechanisms of malignant transformation of normal human cells and tumor formation induced by EBV remain unclear. In this study, we examined and compared the gene expression profiles of EBV-induced lymphomas and normal human lymphocytes of the same origin in SCID mice. By constructing the gene-function relationship network, we preliminarily found that TOP2A, UHRF1, HIST2H2BE, PHGDH, VCL, IGF1R, FOS, SNAI1, PBK, and RNF144B may be the key genes in EBV-induced lymphomas. These findings suggest that the induction of lymphoma by EBV is a complex process that involves multiple genes and pathways.

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