HNK-1 expression pattern in normal and bis-diamine induced malformed developing rat heart: three dimensional reconstruction analysis using computer graphics

1992 ◽  
Vol 186 (4) ◽  
Author(s):  
Hajime Ito ◽  
Keisuke Iwasaki ◽  
Takayoshi Ikeda ◽  
Hiroyuki Sakai ◽  
Isao Shimokawa ◽  
...  
1989 ◽  
Vol 224 (3) ◽  
pp. 443-457 ◽  
Author(s):  
Alfons C. Laan ◽  
Wouter H. Lamers ◽  
Dionysius P. Huijsmans ◽  
Anita Te Kortschot ◽  
Jerry Smith ◽  
...  

1986 ◽  
Vol 3 (4) ◽  
pp. 401-406 ◽  
Author(s):  
Norio Baba ◽  
Shin-Ichi Nakamura ◽  
Isamu Kino ◽  
Koichi Kanaya

Author(s):  
J. Frank ◽  
B. F. McEwen ◽  
M. Radermacher ◽  
C. L. Rieder

The tomographic reconstruction from multiple projections of cellular components, within a thick section, offers a way of visualizing and quantifying their three-dimensional (3D) structure. However, asymmetric objects require as many views from the widest tilt range as possible; otherwise the reconstruction may be uninterpretable. Even if not for geometric obstructions, the increasing pathway of electrons, as the tilt angle is increased, poses the ultimate upper limitation to the projection range. With the maximum tilt angle being fixed, the only way to improve the faithfulness of the reconstruction is by changing the mode of the tilting from single-axis to conical; a point within the object projected with a tilt angle of 60° and a full 360° azimuthal range is then reconstructed as a slightly elliptic (axis ratio 1.2 : 1) sphere.


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