The unipolar brush cells of the rat cerebellar cortex and cochlear nucleus are calretinin-positive: a study by light and electron microscopic immunocytochemistry

AbstractCircuitry of the cerebellar cortex is regionally and functionally specialized. Unipolar brush cells (UBCs), and Purkinje cell (PC) synapses made by axon collaterals in the granular layer, are both enriched in areas that control balance and eye-movement. Here we find a link between these specializations: PCs preferentially inhibit mGluR1-expressing UBCs that respond to mossy fiber inputs with long lasting increases in firing, but PCs do not inhibit mGluR1-lacking UBCs. PCs inhibit about 29% of mGluR1-expressing UBCs by activating GABAA receptors (GABAARs) and inhibit almost all mGluR1-expressing UBCs by activating GABABRs. PC to UBC synapses allow PC output to regulate the input layer of the cerebellar cortex in diverse ways. GABAAR-mediated feedback is fast, unreliable, noisy, and suited to linearizing input-output curves and decreasing gain. Slow GABABR-mediated inhibition allows elevated PC activity to sharpen the input-output transformation of UBCs, and allows dynamic inhibitory feedback of mGluR1-expressing UBCs.

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Chapter 8 The unipolar brush cells of the mammalian cerebellum and cochlear nucleus: cytology and microcircuitry

The Cerebellum: From Structure to Control - Progress in Brain Research ◽

10.1016/s0079-6123(08)63362-2 ◽

1997 ◽

pp. 131-150 ◽

Cited By ~ 85

Author(s):

Enrico Mugnaini ◽

Maria R. Di˜o ◽

Dick Jaarsma

Keyword(s):

Cochlear Nucleus ◽

Brush Cells ◽

Unipolar Brush Cells

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GABA neurons in the superficial layers of the rat dorsal cochlear nucleus: Light and electron microscopic immunocytochemistry

The Journal of Comparative Neurology ◽

10.1002/cne.902350106 ◽

1985 ◽

Vol 235 (1) ◽

pp. 61-81 ◽

Cited By ~ 167

Author(s):

Enrico Mugnaini

Keyword(s):

Cochlear Nucleus ◽

Dorsal Cochlear Nucleus ◽

Electron Microscopic ◽

Electron Microscopic Immunocytochemistry ◽

Gaba Neurons

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Dissociation of doublecortin expression and neurogenesis in unipolar brush cells in the vestibulocerebellum and dorsal cochlear nucleus of the adult rat

Neuroscience ◽

10.1016/j.neuroscience.2014.01.026 ◽

2014 ◽

Vol 265 ◽

pp. 323-331 ◽

Cited By ~ 9

Author(s):

N. Paolone ◽

S. Manohar ◽

S.H. Hayes ◽

K.M. Wong ◽

R.J. Salvi ◽

...

Keyword(s):

Cochlear Nucleus ◽

Dorsal Cochlear Nucleus ◽

Brush Cells ◽

Adult Rat ◽

Unipolar Brush Cells

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Purkinje cell outputs selectively inhibit a subset of unipolar brush cells in the input layer of the cerebellar cortex

eLife ◽

10.7554/elife.68802 ◽

2021 ◽

Vol 10 ◽

Author(s):

Chong Guo ◽

Stephanie Rudolph ◽

Morgan E Neuwirth ◽

Wade G Regehr

Keyword(s):

Purkinje Cell ◽

Cerebellar Cortex ◽

Metabotropic Glutamate Receptor ◽

Mossy Fiber ◽

Brush Cells ◽

Metabotropic Glutamate ◽

Unipolar Brush Cells ◽

Input Layer ◽

Almost All ◽

Control Balance

Circuitry of the cerebellar cortex is regionally and functionally specialized. Unipolar brush cells (UBCs), and Purkinje cell (PC) synapses made by axon collaterals in the granular layer, are both enriched in areas that control balance and eye movement. Here, we find a link between these specializations in mice: PCs preferentially inhibit metabotropic glutamate receptor type 1 (mGluR1)-expressing UBCs that respond to mossy fiber (MF) inputs with long lasting increases in firing, but PCs do not inhibit mGluR1-lacking UBCs. PCs inhibit about 29% of mGluR1-expressing UBCs by activating GABAA receptors (GABAARs) and inhibit almost all mGluR1-expressing UBCs by activating GABAB receptors (GABABRs). PC to UBC synapses allow PC output to regulate the input layer of the cerebellar cortex in diverse ways. Based on optogenetic studies and a small number of paired recordings, GABAAR-mediated feedback is fast and unreliable. GABABR-mediated inhibition is slower and is sufficiently large to strongly influence the input-output transformations of mGluR1-expressing UBCs.

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