Daily fluctuations of nucleoli in neurosecretory cells of the rat supraoptic nucleus

1985 ◽  
Vol 240 (2) ◽  
Author(s):  
Richard Bessone ◽  
Raymond Se�te
Keyword(s):  
Supraoptic Nucleus ◽  
Neurosecretory Cells

scholarly journals ΔFosB in the supraoptic nucleus contributes to hyponatremia in rats with cirrhosis

2012 ◽  
Vol 303 (2) ◽  
pp. R177-R185 ◽  
Author(s):  
J. Thomas Cunningham ◽  
Thekkethil Prashant Nedungadi ◽  
Joseph D. Walch ◽  
Eric J. Nestler ◽  
Helmut B. Gottlieb
Keyword(s):  
Supraoptic Nucleus ◽  
Fluorescent Protein ◽  
Sodium Concentration ◽  
Neurosecretory Cells ◽  
Dominant Negative ◽  
Secretory Cells ◽  
Aav Vector ◽  
Vasopressin Release ◽  
Duct Ligation ◽  
Dilutional Hyponatremia

Bile duct ligation (BDL), a model of hepatic cirrhosis, is associated with dilutional hyponatremia and inappropriate vasopressin release. ΔFosB staining was significantly increased in vasopressin and oxytocin magnocellular neurosecretory cells in the supraoptic nucleus (SON) of BDL rats. We tested the role of SON ΔFosB in fluid retention following BDL by injecting the SON ( n = 10) with 400 nl of an adeno-associated virus (AAV) vector expressing ΔJunD (a dominant negative construct for ΔFosB) plus green fluorescent protein (GFP) (AAV-GFP-ΔJunD). Controls were either noninjected or injected with an AAV vector expressing only GFP. Three weeks after BDL or sham ligation surgery, rats were individually housed in metabolism cages for 1 wk. Average daily water intake was significantly elevated in all BDL rats compared with sham ligated controls. Average daily urine output was significantly greater in AAV-GFP-ΔJunD-treated BDL rats compared with all other groups. Daily average urine sodium concentration was significantly lower in AAV-GFP-ΔJunD-treated BDL rats than the other groups, although average daily sodium excretion was not different among the groups. SON expression of ΔJunD produced a diuresis in BDL rats that may be related to decreased circulating levels of vasopressin or oxytocin. These findings support the view that ΔFosB expression in SON magnocellular secretory cells contribute to dilutional hyponatremia in BDL rats.

Contribution of TRPV1 to the osmosensitivity in the synaptic inputs to the magnocellular neurosecretory cells in the supraoptic nucleus

2009 ◽  
Vol 65 ◽  
pp. S219
Author(s):  
Toru Yokoyama ◽  
Toyoaki Ohbuchi ◽  
Tosihisa Nagatomo ◽  
Yoichi Ueta
Keyword(s):  
Supraoptic Nucleus ◽  
Neurosecretory Cells ◽  
Synaptic Inputs

scholarly journals Centrally administered neuropeptide W-30 activates magnocellular neurosecretory cells in the supraoptic and paraventricular nuclei with neurosecretion in rats

2006 ◽  
Vol 190 (2) ◽  
pp. 213-223 ◽  
Author(s):  
Makoto Kawasaki ◽  
Tatsushi Onaka ◽  
Masamitsu Nakazato ◽  
Jun Saito ◽  
Takashi Mera ◽  
...  
Keyword(s):  
Paraventricular Nucleus ◽  
Arginine Vasopressin ◽  
Supraoptic Nucleus ◽  
Neurosecretory Cells ◽  
Hybridization Histochemistry ◽  
Paraventricular Nuclei ◽  
Fos Protein ◽  
Regulation Of Secretion ◽  
Plasma Arginine

We examined the effects of i.c.v. administration of neuro-peptide W-30 (NPW30) on plasma arginine vasopressin (AVP) and plasma oxytocin (OXT) using RIA. The induction of c-fos mRNA, AVP heteronuclear (hn)RNA, and c-Fos protein (Fos) in the supraoptic nucleus (SON) and paraventricular nucleus (PVN) of rats were also investigated using in situ hybridization histochemistry for c-fos mRNA and AVP hnRNA, and immunohistochemistry for Fos. Both plasma AVP and OXT were significantly increased at 5 and 15 min after i.c.v. administration of NPW30 (2.8 nmol/rat). In situ hybridization histochemistry revealed that the induction of c-fos mRNA and AVP hnRNA in the SON and PVN were significantly increased 15, 30, and 60 min after i.c.v. administration of NPW30 (1.4 nmol/rat). Dual immunostaining for Fos/AVP and Fos/OXT revealed that both AVP-like immunoreactive (LI) cells and OXT-LI cells exhibited nuclear Fos-LI in the SON and PVN, 90 min after i.c.v. administration of NPW30 (2.8 nmol/rat). These results suggest that central NPW30 may be involved in the regulation of secretion of AVP and OXT in the magnocellular neurosecretory cells in the SON and PVN.

Response of supraoptic neurosecretory cells to changes in left atrial distension

1979 ◽  
Vol 236 (5) ◽  
pp. R261-R267
Author(s):  
R. P. Menninger
Keyword(s):  
Left Atrium ◽  
Left Atrial ◽  
Supraoptic Nucleus ◽  
Neurosecretory Cells ◽  
Simultaneous Increase ◽  
Atrial Wall ◽  
Firing Rates ◽  
Series Of Experiments ◽  
Atrial Receptors ◽  
Left Atrial Distension

Acute studies were conducted in cats to determine the effects of small changes in left atrial pressure (LAP) on the activity of antidromically identified neurons in the supraoptic nucleus (SON). Inflation of left atrial balloons reproducibly altered the frequency of 15% of the SON neurons. More than half of these increased frequency, probably due to a simultaneous increase in arterial pressure. In a second series, the firing rates of 20% of the SON neurons tested were inversely related to changes in LAP produced by pumping blood into or out of the left atrium. A third series of experiments were conducted in which the left atrium was directly stretched by pulling on a suture placed in the atrial wall in addition to one of the above methods of changing LAP. Directly stretching the left atrium inhibited over 70% of the SON neurons; this included all the neurons that responded to changes in LAP. The results suggest that these SON neurons have differential sensitivities to inputs from left atrial receptors.

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