pH control for fed-batch culture of Bacillus thuringiensis

1994 ◽  
Vol 8 (7) ◽  
Author(s):  
Jian-Zhong Jong ◽  
Wen-Teng Wu ◽  
Yew-Min Tzeng
Keyword(s):  
Bacillus Thuringiensis ◽  
Batch Culture ◽  
Ph Control ◽  
Fed Batch ◽  
Fed Batch Culture

High-level production of arachidonic acid by fed-batch culture of Mortierella alpina using NH4OH as a nitrogen source and pH control

2005 ◽  
Vol 27 (10) ◽  
pp. 731-735 ◽  
Author(s):  
Byung-Hae Hwang ◽  
Jin-Wook Kim ◽  
Chang-Yeol Park ◽  
Chang-Seo Park ◽  
Yong-Sung Kim ◽  
...  
Keyword(s):  
Arachidonic Acid ◽  
Nitrogen Source ◽  
Batch Culture ◽  
Ph Control ◽  
Mortierella Alpina ◽  
Fed Batch ◽  
Fed Batch Culture ◽  
High Level ◽  
Level Production

Improved Production of Bacillus thuringiensis by Intermittent Fed-Batch Culture with Total Cell Retention

2010 ◽  
Vol 8 (1) ◽  
Author(s):  
Edwin Habeych ◽  
Andrés F González Barrios ◽  
Alexandre Restrepo-Boland ◽  
Sergio Orduz
Keyword(s):  
Bacillus Thuringiensis ◽  
Batch Culture ◽  
Spodoptera Frugiperda ◽  
Total Cell ◽  
Spore Concentration ◽  
Fed Batch ◽  
Cell Retention ◽  
Filtration System ◽  
Fed Batch Culture ◽  
Flow Filtration

Intermittent fed-batch culture with total cell retention was evaluated during Bacillus thuringiensis serovar. kurstaki fermentations aiming to enhance biomass and spore concentration yields while maintaining toxicity. This method is derived from the already reported intermittent fed-batch culture and utilized a tangential flow filtration system for cell retention. After 4 pulses, we obtained a biomass of 74.36 g·L-1 and this biomass displayed a half lethal concentration towards neonate Spodoptera frugiperda larvae of 40.53 ng·cm-2, and spore concentration of 4.6 x 1014 CFU mL-1. On the other hand, volumetric productivity was increased seven-fold compared to batch fermentation. Interestingly, we are not aware of higher spore concentrations reported to date for B. thuringiensis cultures.

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