Cloning of the coat protein gene from beet necrotic yellow vein virus and its expression in sugar beet hairy roots

1991 ◽  
Vol 81 (6) ◽  
pp. 777-782 ◽  
Author(s):  
U. Ehlers ◽  
U. Commandeur ◽  
R. Frank ◽  
J. Landsmann ◽  
R. Koenig ◽  
...  
Keyword(s):  
Coat Protein ◽  
Sugar Beet ◽  
Hairy Roots ◽  
Coat Protein Gene ◽  
Protein Gene ◽  
Yellow Vein

scholarly journals Advances in development of transgenic resistance to beet necrotic yellow vein virus (BNYVV) in sugar beet

Genetika ◽  
2005 ◽  
Vol 37 (3) ◽  
pp. 181-189
Author(s):  
Nevena Nagl ◽  
Ivan Atanasov ◽  
Krasimir Rusanov ◽  
Svetlana Paunovic ◽  
Lazar Kovacev ◽  
...  
Keyword(s):  
Coat Protein ◽  
Sugar Beet ◽  
Coat Protein Gene ◽  
Protein Gene ◽  
Transformation Method ◽  
Yellow Vein ◽  
Bar Gene ◽  
Axillary Shoots ◽  
Viral Cdna

Fragments of viral cDNA containing the coat protein gene of beet necrotic yellow vein virus were cloned in plant transformation vector pCAMBIA3301M with the bar gene as selectable marker. Vector pC3301MCPL carrying coat protein gene with leader sequence, and pC3301MCPS with coat protein gene, were used in Agrobacterium - mediated transformation of sugar beet. The transformation method used was based on the fact that sugar beet develops axillary shoots in in vitro conditions, when placed on media with citokinins. Since this ability is not genotype or ploidy dependant it is widely used for sugar beet vegetative multiplication. Sterile seedlings, with removed cotyledons and lower half of hypocotyl, were used as starting material. After transformation ex-plants were put on micropropagation medium with cephotaxime and phosphinotricyn (ppt), where axillary shoots started to develop. Since concentration of ppt was not selective enough, after two subcultivations it was increased twofold. Only one sample, transformed with pC3301MCPS preserved morphogenetic potential for micropropagatio, and it was tested for presence of COS fragment and bar gene bz PCR with soecific primers.

Molecular Identification and Analysis of Coat Protein Gene of Cucucmber mosaic cucumovirus Sugar Beet Egyptian Isolate

2014 ◽  
Vol 5 (3) ◽  
pp. 70-83 ◽  
Author(s):  
A.A. Megahed ◽  
Kh.A. El- Dougdoug ◽  
B.A. Othman ◽  
S.M. Lashin ◽  
M.D. Hassanin ◽  
...  
Keyword(s):  
Coat Protein ◽  
Sugar Beet ◽  
Molecular Identification ◽  
Coat Protein Gene ◽  
Protein Gene ◽  
Egyptian Isolate

scholarly journals New species emergence via recombination among isolates of the Brazilian tomato infecting Begomovirus complex

2006 ◽  
Vol 41 (8) ◽  
pp. 1329-1332 ◽  
Author(s):  
Alice Kazuko Inoue-Nagata ◽  
Darren Patrick Martin ◽  
Leonardo Silva Boiteux ◽  
Leonardo de Britto Giordano ◽  
Isabel Cristina Bezerra ◽  
...  
Keyword(s):  
New Species ◽  
Coat Protein ◽  
Coat Protein Gene ◽  
Intergenic Region ◽  
Protein Gene ◽  
Nucleotide Sequences ◽  
Yellow Vein ◽  
Streak Virus ◽  
Recombination Analysis ◽  
Leaf Curl Virus

Partial nucleotide sequences of five tomato infecting Begomovirus isolates were determined from DNA-A fragments, corresponding to the 5' region of the replication associated protein gene, the intergenic region and the 5' region of the coat protein gene. Isolate DFM shared 95% identity with Tomato mottle leaf curl virus (TMoLCV), isolates 34, PA-05, and Ta4 were 88% identical to Tomato yellow vein streak virus and isolate DF-BR3 shared 77% identity with TMoLCV. Recombination analysis indicated that isolate DF-BR3 was a chimaera, and it provided evidence that there is a complex and actively recombining population of tomato infecting begomoviruses in Brazil.

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