Transfer of resistance to the beet cyst nematode (Heterodera Schachtii Schm.) from Sinapis alba L. (white mustard) to the Brassica napus L. gene pool by means of sexual and somatic hybridization

1993 ◽  
Vol 85-85 (6-7) ◽  
pp. 688-696 ◽  
Author(s):  
C. L. C. Lelivelt ◽  
E. H. M. Leunissen ◽  
H. J. Frederiks ◽  
J. P. F. G. Helsper ◽  
F. A. Krens
2005 ◽  
Vol 47 (1) ◽  
pp. 84-91 ◽  
Author(s):  
You-Ping WANG ◽  
Karin SONNTAG ◽  
Eicke RUDLOFF ◽  
Jian-Min CHEN

1991 ◽  
Vol 71 (2) ◽  
pp. 397-404 ◽  
Author(s):  
R. P. Bodnaryk ◽  
R. J. Lamb

Seedlings of Brassica napus L. 'Westar' or Sinapis alba L. 'Ochre' grown from seeds of various size were offered to flea beetles, Phyllotreta cruciferae (Goeze) in feeding tests for damage assessment. The proportion of cotyledon area damaged in both species was highest for seedlings grown from small seeds in choice and no-choice feeding tests. The proportion of seedlings killed by flea beetle feeding was also highest in seedlings from small seeds, an effect that was especially pronounced at high beetle densities (10/seedling) where 100% of seedlings from small seeds of B. napus were killed compared to 28.3% of seedlings from big seeds. For S. alba, at 20 beetles/seedling, 45.4% of seedlings from small seeds were killed compared to only 9.1% of seedlings from big seeds. Seedlings grown from big seeds of S. alba tolerated low levels of damage caused by flea beetle feeding or by scissors. No evidence for tolerance was obtained for seedlings from small seeds of S. alba or for seedlings from big or small seeds of B. napus. "Big seeds" appears to be a desirable trait that enhances crucifer seedling resistance to flea beetle attack and results in increased seedling survival. Key words: Brassica napus 'Westar', Sinapis alba 'Ochre', Phyllotreta cruciferae, flea beetle, seed size, resistance


Nematology ◽  
2003 ◽  
Vol 5 (1) ◽  
pp. 17-22 ◽  
Author(s):  
Rainer Rühm ◽  
Elvira Dietsche ◽  
Hans-Joachim Harloff ◽  
Manuela Lieb ◽  
Stephan Franke ◽  
...  

AbstractA kairomone from white mustard, Sinapis alba , that attracts infective juveniles (J2) of the beet cyst nematode, Heterodera schachtii, was extracted from root exudates and enriched by rotary evaporation. The kairomone-containing root exudate fractions were then repeatedly enriched and separated on a combination of octyl and octadecyl columns, leaving a single UV-absorbing HPLC peak. Glucose and fructose were identified by a subsequent HPLC separation in the ion moderated partition chromatography mode. The kairomone appeared in the fructose-containing fraction. As fructose did not cause nematode attraction, the fructose fraction contained at least one further substance. HPLC separations showed that the kairomone shares several properties with fructose and that it is relatively more polar than those compounds giving an UV signal. The repeated reversed-phase and ion moderated partition chromatography separations of single kairomone-containing fractions indicate that the kairomone is composed of one single substance which could not be identified with the methods applied. Gas chromatographic and mass spectrometric analyses revealed the presence of glucose, fructose, myo-inositol, sucrose and xylofuranose/ribofuranose in S. alba root exudates.


Nematology ◽  
2005 ◽  
Vol 7 (6) ◽  
pp. 881-889 ◽  
Author(s):  
Miroslaw Sobczak ◽  
Wladyslaw Golinowski ◽  
Ahmed Soliman

AbstractResponses of two Sinapis alba cultivars (nematode susceptible cv. Albatros and nematode resistant cv. Maxi) to infection with the root-parasitic nematode Heterodera schachtii were investigated at the anatomical and cytological levels. In the susceptible cv. Albatros, male and female juveniles develop. Syncytia associated with female juveniles are induced in the procambium and they preferentially incorporate procambial cells abutting xylem vessels. The syncytial elements are greatly enlarged and interconnected by wide cell wall openings. Syncytia associated with male juveniles are induced in the pericycle and they incorporate primarily greatly enlarged pericyclic cells and sometimes less enlarged procambial cells. In the resistant cv. Maxi, most of juveniles remain as secondstage juveniles while some develop into males. Their syncytia are induced in and involve pericyclic cells only. Syncytia induced in cv. Maxi by males differ from those induced in susceptible cv. Albatros by less enlargement of nuclei, the presence of remnants of the central vacuole, and a marked presence of rough endoplasmic reticulum. At least three different defence responses could be discriminated in cv. Maxi: i) a hypersensitive response of vascular cylinder cells during/after nematode invasion; ii) degeneration of syncytia induced in the procambium; and iii) formation of a layer of necrotic cells between syncytium and xylem vessels.


1988 ◽  
Vol 75 (4) ◽  
pp. 546-552 ◽  
Author(s):  
C. Primard ◽  
F. Vedel ◽  
C. Mathieu ◽  
G. Pelletier ◽  
A. M. Ch�vre

1990 ◽  
Vol 104 (1) ◽  
pp. 26-33 ◽  
Author(s):  
Van L. Ripley ◽  
P. G. Arnison

2000 ◽  
Vol 267 (21) ◽  
pp. 6486-6492 ◽  
Author(s):  
Margherita Ruoppolo ◽  
Angela Amoresano ◽  
Piero Pucci ◽  
Stefano Pascarella ◽  
Fabio Polticelli ◽  
...  

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