Repetitive somatic embryogenesis from peanut cultures in liquid medium

1992 ◽  
Vol 11 (3) ◽  
Author(s):  
RichardE. Durham ◽  
WayneA. Parrott
Keyword(s):  
Somatic Embryogenesis ◽  
Liquid Medium ◽  
Repetitive Somatic Embryogenesis

Somatic embryogenesis of Cyclamen persicum in liquid medium

1995 ◽  
Vol 94 (4) ◽  
pp. 605-612 ◽  
Author(s):  
Marc Kreuger ◽  
Erik Postma ◽  
Yvon Brouwer ◽  
Gerrit-Jan van Holst
Keyword(s):  
Somatic Embryogenesis ◽  
Liquid Medium ◽  
Cyclamen Persicum

Repetitive somatic embryogenesis from leaves of the medicinal plant Petiveria alliacea L.

2013 ◽  
Vol 115 (3) ◽  
pp. 385-393 ◽  
Author(s):  
L. Cantelmo ◽  
B. O. Soares ◽  
L. P. Rocha ◽  
J. A. Pettinelli ◽  
C. H. Callado ◽  
...  
Keyword(s):  
Somatic Embryogenesis ◽  
Medicinal Plant ◽  
Petiveria Alliacea ◽  
Repetitive Somatic Embryogenesis

Repetitive somatic embryogenesis in Medicago truncatula ssp. Narbonensis and M. truncatula Gaertn cv. Jemalong

1999 ◽  
Vol 18 (5) ◽  
pp. 398-405 ◽  
Author(s):  
L. O. das Neves ◽  
S. R. L. Duque ◽  
J. S. de Almeida ◽  
P. S. Fevereiro
Keyword(s):  
Somatic Embryogenesis ◽  
Medicago Truncatula ◽  
Repetitive Somatic Embryogenesis

scholarly journals Induction of cassava somatic embryogenesis in liquid medium associated to floating membrane rafts

2000 ◽  
Vol 43 (1) ◽  
Author(s):  
Elizabete Keiko Takahashi ◽  
Adilson Kenji Kobayashi ◽  
Luiz Gonzaga Esteves Vieira
Keyword(s):  
Somatic Embryogenesis ◽  
Liquid Medium ◽  
Manihot Esculenta ◽  
Solid Medium ◽  
Young Leaf ◽  
Membrane Rafts ◽  
Manihot Esculenta Crantz ◽  
Lower Response ◽  
Culture Systems

The objective of this study was to examine the effect of two culture systems, liquid medium associated to floating membranes and solid medium, both supplemented with different concentrations of 2,4-D, in the induction of somatic embryogenesis of cassava (Manihot esculenta Crantz). Only 28% of the young leaf lobes (with 9 µM 2,4-D) were induced to form organized embryogenic structures (OES) with membrane rafts, compared to 50% of the explants presenting this type of tissue in solid medium with 36 µM of 2,4-D. Despite the lower response observed in liquid medium with membrane, the amount of OES/explant in all 2,4-D concentrations was higher than solid medium. Based on the results and considering the high cost of the membrane rafts, this system was not distinctly superior than solid medium for inducing somatic embryogenesis in cassava.

Morpho-histological and ultrastructural study on direct somatic embryogenesis of Capsicum chinense Jacq. in liquid medium

2012 ◽  
Vol 7 (3) ◽  
pp. 277-286 ◽  
Author(s):  
Susana A. Avilés-Viñas ◽  
Carlos A. Lecona-Guzmán ◽  
Adriana Canto-Flick ◽  
Stephanie López-Erosa ◽  
Nancy Santana-Buzzy
Keyword(s):  
Somatic Embryogenesis ◽  
Liquid Medium ◽  
Ultrastructural Study ◽  
Direct Somatic Embryogenesis ◽  
Capsicum Chinense

scholarly journals Somatic Embryogenesis in Arabidopsis thaliana Is Facilitated by Mutations in Genes Repressing Meristematic Cell Divisions

Genetics ◽  
1998 ◽  
Vol 149 (2) ◽  
pp. 549-563
Author(s):  
Andreas P Mordhorst ◽  
Keete J Voerman ◽  
Marijke V Hartog ◽  
Ellen A Meijer ◽  
Jacques van Went ◽  
...  
Keyword(s):  
Somatic Embryogenesis ◽  
Liquid Medium ◽  
Cell Cultures ◽  
Egg Cell ◽  
Zygotic Embryos ◽  
Additive Effects ◽  
Additional Advantage ◽  
Embryogenic Cell ◽  
Immature Zygotic Embryos

Abstract Embryogenesis in plants can commence from cells other than the fertilized egg cell. Embryogenesis initiated from somatic cells in vitro is an attractive system for studying early embryonic stages when they are accessible to experimental manipulation. Somatic embryogenesis in Arabidopsis offers the additional advantage that many zygotic embryo mutants can be studied under in vitro conditions. Two systems are available. The first employs immature zygotic embryos as starting material, yielding continuously growing embryogenic cultures in liquid medium. This is possible in at least 11 ecotypes. A second, more efficient and reproducible system, employing the primordia timing mutant (pt allelic to hpt, cop2, and amp1), was established. A significant advantage of the pt mutant is that intact seeds, germinated in 2,4-dichlorophenoxyacetic acid (2,4-D) containing liquid medium, give rise to stable embryonic cell cultures, circumventing tedious hand dissection of immature zygotic embryos. pt zygotic embryos are first distinguishable from wild type at early heart stage by a broader embryonic shoot apical meristem (SAM). In culture, embryogenic clusters originate from the enlarged SAMs. pt somatic embryos had all characteristic embryo pattern elements seen in zygotic embryos, but with higher and more variable numbers of cells. Embryogenic cell cultures were also established from seedling, of other mutants with enlarged SAMs, such as clavata (clv). pt clv double mutants showed additive effects on SAM size and an even higher frequency of seedlings producing embryogenic cell lines. pt clv double mutant plants had very short fasciated inflorescence stems and additive effects on the number of rosette leaves. This suggests that the PT and CLV genes act in independent pathways that control SAM size. An increased population of noncommitted SAM cells may be responsible for facilitated establishment of somatic embryogenesis in Arabidopsis.

SOMATIC EMBRYOGENESIS IN CELL SUSPENSION CULTURE OF COWPEA (VIGNA UNGUICULATA (L.) WALP)

1995 ◽  
Vol 43 (4) ◽  
pp. 385-390 ◽  
Author(s):  
S. Kulothungan ◽  
A. Ganapathi ◽  
A. Shajahan ◽  
K. Kathiravan
Keyword(s):  
Somatic Embryogenesis ◽  
Liquid Medium ◽  
Vigna Unguiculata ◽  
Somatic Embryos ◽  
Leaf Explants ◽  
Dichlorophenoxyacetic Acid ◽  
Ms Medium ◽  
2,4 Dichlorophenoxyacetic Acid ◽  
Seedling Leaf ◽  
Cotyledonary Stage

Embryogenic callus was induced from seedling leaf explants of cowpea (Vigna unguiculata (L.) Walp. cv. C152 on Murashige and Skoog (MS) medium containing 2.0 mg 1−1 2,4-dichlorophenoxyacetic acid (2,4-D). The maximum frequency of somatic embryogenesis was noticed when this callus was transferred to MS liquid medium supplemented with 2 mg 1−1 2,4-D. Further studies on ontogeny of somatic embryos showed that the cells destined to become somatic embryos divided into spherical or filamentous proembryos. Subsequent divisions in the proembryo led to globular, heart, torpedo-shaped, and cotyledonary-stage somatic embryos. Tiny plantlets were obtained by transferring the cotyledonary-stage somatic embryos to MS liquid medium containing 0.5 mg 1−1 2,4-D.

Somatic embryogenesis of Cyclamen persicum in liquid medium

1995 ◽  
Vol 94 (4) ◽  
pp. 605-612 ◽  
Author(s):  
Marc Kreuger ◽  
Erik Postma ◽  
Yvon Brouwer ◽  
Gerrit-Jan Holst
Keyword(s):  
Somatic Embryogenesis ◽  
Liquid Medium ◽  
Cyclamen Persicum
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