Morpho-histological and ultrastructural study on direct somatic embryogenesis of Capsicum chinense Jacq. in liquid medium

2012 ◽  
Vol 7 (3) ◽  
pp. 277-286 ◽  
Author(s):  
Susana A. Avilés-Viñas ◽  
Carlos A. Lecona-Guzmán ◽  
Adriana Canto-Flick ◽  
Stephanie López-Erosa ◽  
Nancy Santana-Buzzy
Keyword(s):  
Somatic Embryogenesis ◽  
Liquid Medium ◽  
Ultrastructural Study ◽  
Direct Somatic Embryogenesis ◽  
Capsicum Chinense

scholarly journals Somatic Embryogenesis in Habanero Pepper (C. chinense Jacq.) From Cell Suspensions

HortScience ◽  
2007 ◽  
Vol 42 (2) ◽  
pp. 329-333 ◽  
Author(s):  
Patricia Yolanda Zapata-Castillo ◽  
Adriana-Canto Flick ◽  
Guadalupe López-Puc ◽  
Anabel Solís-Ruiz ◽  
Felipe Barahona-Pérez ◽  
...  
Keyword(s):  
Somatic Embryogenesis ◽  
Liquid Medium ◽  
Embryogenic Callus ◽  
Culture Media ◽  
Continuous Light ◽  
Cell Suspensions ◽  
Ms Medium ◽  
Capsicum Chinense ◽  
Habanero Pepper ◽  
Different Types

To induce the somatic embryogenesis of Habanero pepper, different culture media and different types of explants (node, internode, hypocotyl, half seeds, and fruit segments) were evaluated. For the induction of embryogenic callus, 9.05 μm of 2,4-dichlorofenoxiacetic acid, 3% sucrose, and 0.8% gelrite were added to the basic MS medium over a period of 30 days at 25 ± 2 °C under continuous light (40–50 μmol·m2·s−1). Once the callus formed, they were transferred to liquid medium using the same induction formulation. Somatic embryogenesis only occurred from explants of hypocotyl and in the presence of 3.4 μm thidiazuron. This constitutes the first proposal of a protocol for the “induction of somatic embryogenesis in Habanero pepper (Capsicum chinense Jacq.) from cell suspensions.”

scholarly journals Direct Somatic Embryogenesis: A Highly Efficient Protocol for In Vitro Regeneration of Habanero Pepper (Capsicum chinense Jacq.)

HortScience ◽  
2006 ◽  
Vol 41 (7) ◽  
pp. 1645-1650 ◽  
Author(s):  
Guadalupe López-Puc ◽  
Adriana Canto-Flick ◽  
Felipe Barredo-Pool ◽  
Patricia Zapata-Castillo ◽  
María del C. Montalvo-Peniche ◽  
...  
Keyword(s):  
Somatic Embryogenesis ◽  
Somatic Embryos ◽  
Culture Media ◽  
Callus Formation ◽  
Direct Somatic Embryogenesis ◽  
Zygotic Embryos ◽  
Capsicum Chinense ◽  
Skoog Medium ◽  
Habanero Pepper ◽  
Murashige And Skoog Medium

To induce somatic embryogenesis in habanero pepper (Capsicum chinense Jacq.), the cultivar BVll-03, belonging to the red type, was used. Different explants were evaluated, as were different culture media, the composition of which varied in the content of plant growth regulators. Results showed the formation of somatic embryos from cotyledons, zygotic embryos, germinated zygotic embryos, hypocotyls, and cotyledonary leaves. Explants were cultured on Murashige and Skoog medium supplemented with 2,4-D (9.05 μm). The somatic embryos always formed directly from the explant, without callus formation, and the greatest efficiency was obtained when segments of hypocotyls were cultured, obtaining 175 ± 20 somatic embryos per explant. Only the somatic embryos obtained on Murashige and Skoog medium containing 2,4-D (9.05 μm) and treated with abscisic acid (ABA) (1.89 μm) before their transfer to the germination media (Murashige and Skoog + 1.1 μm GA3) emitted their radicule and expanded their cotyledonary leaves (60%), whereas the remaining embryos did not achieve germination because of different causes (abnormalities, delayed development). Not only is this protocol of somatic embryogenesis the first to be reported for this species (C. chinense Jacq.), but it is also the most efficient reported so far, within the Capsicum genus.

Somatic embryogenesis of Cyclamen persicum in liquid medium

1995 ◽  
Vol 94 (4) ◽  
pp. 605-612 ◽  
Author(s):  
Marc Kreuger ◽  
Erik Postma ◽  
Yvon Brouwer ◽  
Gerrit-Jan van Holst
Keyword(s):  
Somatic Embryogenesis ◽  
Liquid Medium ◽  
Cyclamen Persicum

scholarly journals Influence of Abscisic Acid and Sucrose on Somatic Embryogenesis in CactusCopiapoa tenuissimaRitt. formamostruosa

2013 ◽  
Vol 2013 ◽  
pp. 1-7 ◽  
Author(s):  
J. Lema-Rumińska ◽  
K. Goncerzewicz ◽  
M. Gabriel
Keyword(s):  
Somatic Embryogenesis ◽  
Abscisic Acid ◽  
Somatic Embryos ◽  
Sucrose Concentration ◽  
Turgor Pressure ◽  
Direct Somatic Embryogenesis ◽  
Fresh Weight ◽  
High Concentration ◽  
Globular Stage ◽  
Indirect Somatic Embryogenesis

Having produced the embryos of cactusCopiapoa tenuissimaRitt. formamonstruosaat the globular stage and callus, we investigated the effect of abscisic acid (ABA) in the following concentrations: 0, 0.1, 1, 10, and 100 μM on successive stages of direct (DSE) and indirect somatic embryogenesis (ISE). In the indirect somatic embryogenesis process we also investigated a combined effect of ABA (0, 0.1, 1 μM) and sucrose (1, 3, 5%). The results showed that a low concentration of ABA (0-1 μM) stimulates the elongation of embryos at the globular stage and the number of correct embryos in direct somatic embryogenesis, while a high ABA concentration (10–100 μM) results in growth inhibition and turgor pressure loss of somatic embryos. The indirect somatic embryogenesis study in this cactus suggests that lower ABA concentrations enhance the increase in calli fresh weight, while a high concentration of 10 μM ABA or more changes calli color and decreases its proliferation rate. However, in the case of indirect somatic embryogenesis, ABA had no effect on the number of somatic embryos and their maturation. Nevertheless, we found a positive effect of sucrose concentration for both the number of somatic embryos and the increase in calli fresh weight.

scholarly journals The effect of auxin 2,4-D and cytokinin 2-ip on direct somatic embryogenesis formation of Coffea arabica L. leaf explant

2011 ◽  
Vol 27 (2) ◽  
Author(s):  
Rina Arimarsetiowati
Keyword(s):  
Tissue Culture ◽  
Somatic Embryogenesis ◽  
Coffea Arabica ◽  
Callus Formation ◽  
Culture Technique ◽  
Plant Production ◽  
Direct Somatic Embryogenesis ◽  
Arabica Coffee ◽  
Randomized Design ◽  
Coffea Arabica L

One of the propagation technique for coffee plant production is tissue culture. Tissue culture technique for Coffea arabica L. faces some problems, mainly in the planlet formation regenerated from explants. The objective of this experiment was to examine the effect 2,4-D and 2-ip combination on the formation of direct somatic embryogenesis of Coffea arabica L. in leaves explant. Auxin (2,4-D) and cytokinin (2-ip) concentrations of, respectively, 1; 5 µM and 5; 10; 15; 20 were used as treatments. This research was conducted using completely randomized design with 10 replications. Observation to induce somatic embryos was done by quantitatively on number of callus from explant and number of embryogenic callus. Beside that, observation by qualitative descriptive was also done on deve lopment of embryogenesis. The results showed that Arabica coffee leaves explant of AS 2K clones could be induced in all medium combination except 5µM 2,4-D and 20µM 2-ip combination. Arabica coffee leaves explant of S 795, Sigararutang and AS 1 varieties could be induced in all medium combination. The highest frequency of callus formation was found in AS 2K, Sigararutang and AS 1 varieties on medium containing 1µM 2,4-D in combination with 10µM 2-ip, whereas for the S 795 variety on medium containing 5µM 2,4-D in combination with 10µM 2-ip. The highest frequency of embriogenic callus in all Arabica coffee variety could be reached on medium containing 5µM 2,4-D in combination with 15µM 2-ip. Key words : Coffea arabica L., somatic embryogenesis, 2,4-D, 2-ip, tissue culture, leaves, callus embryogenic.

Direct Somatic Embryogenesis in Coffea canephora

2005 ◽  
pp. 111-118 ◽  
Author(s):  
Francisco Quiroz-Figueroa ◽  
Miriam Monforte-González ◽  
Rosa M. Galaz-Ávalos ◽  
Victor M. Loyola-Vargas
Keyword(s):  
Somatic Embryogenesis ◽  
Coffea Canephora ◽  
Direct Somatic Embryogenesis

scholarly journals Rapid genotype “independent” Zea mays L. (maize) transformation via direct somatic embryogenesis

2018 ◽  
Vol 54 (3) ◽  
pp. 240-252 ◽  
Author(s):  
Keith Lowe ◽  
Mauricio La Rota ◽  
George Hoerster ◽  
Craig Hastings ◽  
Ning Wang ◽  
...  
Keyword(s):  
Zea Mays ◽  
Somatic Embryogenesis ◽  
Zea Mays L ◽  
Direct Somatic Embryogenesis ◽  
Maize Transformation
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