In vitro regeneration of onion through repetitive somatic embryogenesis

1997 ◽  
Vol 39 (4) ◽  
pp. 499-506 ◽  
Author(s):  
M.M. Saker
2011 ◽  
Vol 108 (8) ◽  
pp. 1463-1475 ◽  
Author(s):  
D. A. Steinmacher ◽  
M. P. Guerra ◽  
K. Saare-Surminski ◽  
R. Lieberei

2006 ◽  
Vol 2 (2) ◽  
pp. 146-151 ◽  
Author(s):  
R.O. Oduor ◽  
E.N.M. Njagi ◽  
S. Ndung` u ◽  
J.S. Machuka

2006 ◽  
pp. 489-494
Author(s):  
S. Sharry ◽  
W. Abedini ◽  
S. Lede ◽  
J.L. Cabrera Ponce ◽  
L. Herrera Estrella ◽  
...  

2008 ◽  
Vol 11 (5) ◽  
pp. 726-732 ◽  
Author(s):  
Fotso . ◽  
Oumar . ◽  
Niemenak Nicolas ◽  
Donfagsiteli Tchinda Ne ◽  
Omokolo Ndoumou De

2015 ◽  
Vol 24 (2) ◽  
pp. 247-262 ◽  
Author(s):  
El Sawy A Mohamed ◽  
Amina Gomaa ◽  
Nancy Danial

Better results were obtained when stigma explants of variegated lemon and citron were used. After ten months, somatic embryos developed into plantlets at a frequency ranged from 13.3 for lime to 66.7% for lemon. Virus presence was tested by ELISA and RT?PCR. The results indicated that the plantlets regenerated through somatic embryogenesis are CTV?free. RAPD analysis was used to asses the genetic stability of plantlets as compared to the mother plants. The results indicated that most plantlets belong to the respective mother plants and the polymorphism percentage was genotype and explant?dependant.Plant Tissue Cult. & Biotech. 24(2): 247-262, 2014 (December


2006 ◽  
Vol 9 (3) ◽  
pp. 0-0 ◽  
Author(s):  
Sandra Sharry ◽  
Jose Luis Cabrera Ponce ◽  
Luis Herrera Estrella ◽  
Rosa Maria Rangel Cano ◽  
Silvia Lede ◽  
...  

2019 ◽  
Author(s):  
Zheng Liu ◽  
Xiao-Xia Ge ◽  
Xiao-Meng Wu ◽  
Wen-Wu Guo

Abstract Background In citrus, genetic improvement via biotechnology is hindered by the obstacle of in vitro regeneration via somatic embryogenesis (SE). Although a few of B3 transcription factors are reported to regulate embryogenesis, little is known about the possible roles of B3 superfamily during SE especially in citrus. Results In this study, a total of 72 (CsB3) and 69 (CgB3) putative B3 superfamily members were identified in the sweet orange (Citrus sinensis) and pummelo (C. grandis) genomes, respectively, each comprised four gene families and 14 phylogenetic classes. The B3 genes were unevenly distributed over citrus chromosomes and other non-anchored scaffolds. Genome duplication analysis indicated that the segmental and tandem duplication events have significantly contributed to the expansion of the citrus B3 superfamily. The evolutionary relationships among the B3 family members and their putative functions were deduced based on the results of phylogenetic analysis. Furthermore, transcriptomic analysis showed that citrus B3 genes have differential expression levels in various tissues, suggesting distinct biological roles of different members. Expression analysis revealed that the B3 superfamily members showed four types of expression profiles during SE in citrus and may play functional roles during SE, especially at late SE stages. Of them, CsARF19 is specifically expressed in sweet orange and at markedly higher levels in the embryogenic callus (EC), implying its possible involvement in EC initiation. Conclusions This study provides a genome-wide analysis of citrus B3 superfamily, including its genome organization, evolutionary features and expression profiles, which contributes to a better understanding of the B3 genes in citrus and their association with SE.


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