Repetitive somatic embryogenesis in Medicago truncatula ssp. Narbonensis and M. truncatula Gaertn cv. Jemalong

The induction of plant somatic embryogenesis is often a limiting step for plant multiplication and genetic manipulation in numerous crops. It depends on multiple signaling developmental processes involving phytohormones and the induction of specific genes. The WUSCHEL gene (WUS) is required for the production of plant embryogenic stem cells. To explore a different approach to induce somatic embryogenesis, we have investigated the effect of the heterologous ArabidopsisWUS gene overexpression under the control of the jasmonate responsive vsp1 promoter on the morphogenic responses of Medicago truncatula explants. WUS expression in leaf explants increased callogenesis and embryogenesis in the absence of growth regulators. Similarly, WUS expression enhanced the embryogenic potential of hairy root fragments. The WUS gene represents thus a promising tool to develop plant growth regulator-free regeneration systems or to improve regeneration and transformation efficiency in recalcitrant crops.

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A proteomics study of the induction of somatic embryogenesis in Medicago truncatula using 2DE and MALDI-TOF/TOF

Physiologia Plantarum ◽

10.1111/j.1399-3054.2012.01633.x ◽

2012 ◽

Vol 146 (2) ◽

pp. 236-249 ◽

Cited By ~ 24

Author(s):

André M. Almeida ◽

José R. Parreira ◽

Romana Santos ◽

Ana Sofia Duque ◽

Rita Francisco ◽

...

Keyword(s):

Somatic Embryogenesis ◽

Medicago Truncatula ◽

Maldi Tof ◽

Proteomics Study

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Repetitive somatic embryogenesis from leaves of the medicinal plant Petiveria alliacea L.

Plant Cell Tissue and Organ Culture (PCTOC) ◽

10.1007/s11240-013-0370-7 ◽

2013 ◽

Vol 115 (3) ◽

pp. 385-393 ◽

Cited By ~ 8

Author(s):

L. Cantelmo ◽

B. O. Soares ◽

L. P. Rocha ◽

J. A. Pettinelli ◽

C. H. Callado ◽

...

Keyword(s):

Somatic Embryogenesis ◽

Medicinal Plant ◽

Petiveria Alliacea ◽

Repetitive Somatic Embryogenesis

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Plant Regeneration from Cultured Medicago truncatula with Particular Reference to Abscisic Acid and Light Treatments

Australian Journal of Botany ◽

10.1071/bt96138 ◽

1998 ◽

Vol 46 (1) ◽

pp. 151 ◽

Cited By ~ 23

Author(s):

K. E. Nolan ◽

R. J. Rose

Keyword(s):

Acetic Acid ◽

Somatic Embryogenesis ◽

Abscisic Acid ◽

Plant Regeneration ◽

Medicago Truncatula ◽

Callus Induction ◽

Naphthalene Acetic Acid ◽

Marked Inhibition ◽

Embryo Formation ◽

Auxin Concentration

Medicago truncatula (Jemalong 2HA) can be regenerated by somatic embryogenesis utilising 1-naphthalene acetic acid (NAA) and 6-benzylaminopurine (BAP). There is a requirement for both NAA and BAP for callus induction and embryo formation. There is no requirement for a drop in auxin concentration to induce embryos. Abscisic acid (ABA) when present with NAA and BAP during embryo formation at a concentration of 1 µM, increases the number of embryos per callus. The ABA treatment stimulates embryo numbers in both light and darkness. The conversion efficiency of embryo to plant is unchanged irrespective of the presence of ABA during embryo formation, indicating that ABA does not improve the regeneration of the embryos once formed. Importantly, the presence of light in the embryo formation period causes a marked inhibition of embryo conversion.

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